Ultrasonic Welding of Chemical Optical Sensors Supporting O 2 , pH and CO 2 Imaging in Microfluidic Systems

Krabbe, S.; Achatz, D.E.; Nieradzik, T.; Gerhardy, C.; Schomburg, Werner Karl

doi:10.1016/j.proeng.2015.08.736

Cited by 5 publications

(2 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…23 Fluorescent and luminescent pH sensors have been applied in microfluidic chips mainly for cell culture and monitoring of other bioprocesses and could be incorporated with a variety of different microfabrication techniques. [24][25][26][27][28][29][30][31][32] As with other variations of electrophoresis, one mode of miniaturized FFE is microfluidic free-flow isoelectric focusing (μFFIEF), where compounds are separated in a pH gradient according to their isoelectric point (pI). [33][34][35][36][37] IEF offers an alternative dimension for separation and the pI offers a means for determination or assignment of a particular compound.…”

Section: Introductionmentioning

confidence: 99%

Continuous on-chip fluorescence labelling, free-flow isoelectric focusing and marker-free isoelectric point determination of proteins and peptides

et al. 2016

View full text Add to dashboard Cite

We present a microfluidic platform that contains a micro flow reactor for on-chip biomolecule labelling that is directly followed by a separation bed for continuous free-flow electrophoresis and has an integrated hydrogel-based near-infrared fluorescent pH sensor layer. Using this assembly, labelling of protein and peptide mixtures, their separation via free-flow isoelectric focusing and the determination of the isoelectric point (pI) of the separated products via the integrated sensor layer could be carried out within typically around 5 minutes. Spatially-resolved immobilization of fluidic and sensing structures was carried out via multistep photolithography. The assembly was characterized and optimized with respect to their fluidic and pH sensing properties and applied in the IEF of model proteins, peptides and a tryptic digest from physalaemine. We have therefore realized continuous sample preparation and preparative separation, analyte detection, process observation and analyte assignment capability based on pI on a single platform the size of a microscope slide.

show abstract

Section: Introductionmentioning

confidence: 99%

Continuous on-chip fluorescence labelling, free-flow isoelectric focusing and marker-free isoelectric point determination of proteins and peptides

et al. 2016

View full text Add to dashboard Cite

show abstract

“…In recent decades, industries have generally embraced ultrasonic welding of thermoplastics and it is still widely recognised as a very efficient technology that results in no polymer degradation. Ultrasonic welding is utilised in the packaging industry, for automotive panels, textiles, appliance manufacturing, etc [1][2][3][4]. The process of joining the composites is crucial in the manufacturing process of fibre-reinforced thermoplastic composites for structural purposes.…”

Section: Introductionmentioning

confidence: 99%

Welding analysis and optimization of ultra- sonic welding in HDPE-5%PBI composite by CODAS decision-making approach

Рамеш¹,

Panneerselvam²

2022

Phys. Scr.

View full text Add to dashboard Cite

Engineering plastics are increasingly being used in structural and non-structural applications. As the need for plastics grows, so do the joining requirements. Ultrasonic welding is one of the favoured processes for polymer composites among numerous approaches, especially for bonding plates, automotive panel boards and couplings due to its joint quality and low cost. The High-density polyethylene (HDPE) matrix was reinforced with 5 wt.% of polybenzimidazole (PBI) fibre by twin screw extrusion process and welding samples were prepared using injection moulding. In this study, Ultrasonic welding was used to connect 4-mm-thick lapped HDPE-5wt. % PBI composites. Taguchi-based L27 orthogonal array was employed for experiments to study the lateral shear strength (LSS) and Shore-D-Hardness for the input parameters (i) weld duration (30,45 and 60 ms), (ii) frequency (19,20 and 21 Hz) and (iii) horn pressure (1.5,2 and 2.5 MPa). The relevance of the parameters affecting shear strength and hardness of weld was studied using analysis of variance (ANOVA). ANOVA results shows the frequency (Hz) and weld time (ms) had contributed 32.4% and 31.6% in determining shear strength and weld time (ms) contributes 45% for shore D hardness. The ultrasonic welding process was carried out, and the multi-criteria decision-making (MCDM) approach was used to optimise the process parameters. From CODAS assessment the relative evaluation score of 2.444 was achieved by the twenty fifth experiment having the input parameters (IP) at 60 ms welding time, frequency of 21 Hz and pressure at 2.5 MPa considering each output factor with an entropy of 0.5. Fractography and weld morphology of welded joints were investigated using scanning electron microscope (SEM).

show abstract

Continuous Monitoring of Volatile Organic Compounds in the Ear: The Development of a Headphone-Type Biosensor

Toma,

Iitani,

Arakawa

et al. 2024

Wearable Biosensing in Medicine and Healthcare

View full text Add to dashboard Cite

Ultrasonic Welding of Chemical Optical Sensors Supporting O 2 , pH and CO 2 Imaging in Microfluidic Systems

Cited by 5 publications

References 2 publications

Continuous on-chip fluorescence labelling, free-flow isoelectric focusing and marker-free isoelectric point determination of proteins and peptides

Continuous on-chip fluorescence labelling, free-flow isoelectric focusing and marker-free isoelectric point determination of proteins and peptides

Welding analysis and optimization of ultra- sonic welding in HDPE-5%PBI composite by CODAS decision-making approach

Continuous Monitoring of Volatile Organic Compounds in the Ear: The Development of a Headphone-Type Biosensor

Contact Info

Product

Resources

About