“…Ultrasonication leads to pressure waves of ultrasonication frequency in the liquid medium. The amplitude of these waves is higher at higher power inputs 32 . At sufficiently high-power intensities, ultrasonication has been known to destroy microorganisms and enzymes in food and break down microstructures 32 – 34 .…”
Section: Resultsmentioning
confidence: 93%
“…The amplitude of these waves is higher at higher power inputs 32 . At sufficiently high-power intensities, ultrasonication has been known to destroy microorganisms and enzymes in food and break down microstructures 32 – 34 . Power level is one of the critical factors affecting the efficiency of ultrasound treatment.…”
Section: Resultsmentioning
confidence: 93%
“…For example, the frequency influences the formation and size of cavitation bubbles. At higher frequencies, the acoustic cycle is shorter, giving less time for cavitation bubble formation; therefore, more bubbles with smaller sizes are generated and collapse with less energy 32 – 34 .…”
This study investigates the synergistic effect of ultrasonication and antimicrobial action of antimicrobial peptide cecropin P1 on the inactivation of Escherichia coli O157:H7 in a cylindrical ultrasonication system. The inactivation of E. coli at pH 7.4 was performed using: ultrasonication (14, 22, and 47 kHz), cecropin P1 (20 µg/mL), and a combination of both. We found the treatment at 22 kHz, 8W for 15 min of exposure and a combination of ultrasound at higher frequency (47 kHz, 8 W) and cecropin P1 for one minute of exposure were more efficient, reducing the cell density by six orders of magnitude, compared to individual treatments (ultrasound or cecropin P1 only). Dye leakage studies and transmission electron microscopy further validated these results. A continuous flow system was designed to demonstrate synergism of ultrasonication with antimicrobial peptide Cecropin P1 in the inactivation of E. coli; synergism was shown to be more at higher ultrasonication frequencies and power levels. Acoustic cavitation by ultrasonic treatment could drastically improve microbial deactivation by antimicrobial peptides cecropin P1 by increasing their ability for pore formation in cell membranes. A continuous ultrasonication and antimicrobial peptides system can lead to an energy-efficient and economical sterilization system for food safety applications.
“…Ultrasonication leads to pressure waves of ultrasonication frequency in the liquid medium. The amplitude of these waves is higher at higher power inputs 32 . At sufficiently high-power intensities, ultrasonication has been known to destroy microorganisms and enzymes in food and break down microstructures 32 – 34 .…”
Section: Resultsmentioning
confidence: 93%
“…The amplitude of these waves is higher at higher power inputs 32 . At sufficiently high-power intensities, ultrasonication has been known to destroy microorganisms and enzymes in food and break down microstructures 32 – 34 . Power level is one of the critical factors affecting the efficiency of ultrasound treatment.…”
Section: Resultsmentioning
confidence: 93%
“…For example, the frequency influences the formation and size of cavitation bubbles. At higher frequencies, the acoustic cycle is shorter, giving less time for cavitation bubble formation; therefore, more bubbles with smaller sizes are generated and collapse with less energy 32 – 34 .…”
This study investigates the synergistic effect of ultrasonication and antimicrobial action of antimicrobial peptide cecropin P1 on the inactivation of Escherichia coli O157:H7 in a cylindrical ultrasonication system. The inactivation of E. coli at pH 7.4 was performed using: ultrasonication (14, 22, and 47 kHz), cecropin P1 (20 µg/mL), and a combination of both. We found the treatment at 22 kHz, 8W for 15 min of exposure and a combination of ultrasound at higher frequency (47 kHz, 8 W) and cecropin P1 for one minute of exposure were more efficient, reducing the cell density by six orders of magnitude, compared to individual treatments (ultrasound or cecropin P1 only). Dye leakage studies and transmission electron microscopy further validated these results. A continuous flow system was designed to demonstrate synergism of ultrasonication with antimicrobial peptide Cecropin P1 in the inactivation of E. coli; synergism was shown to be more at higher ultrasonication frequencies and power levels. Acoustic cavitation by ultrasonic treatment could drastically improve microbial deactivation by antimicrobial peptides cecropin P1 by increasing their ability for pore formation in cell membranes. A continuous ultrasonication and antimicrobial peptides system can lead to an energy-efficient and economical sterilization system for food safety applications.
“…Pulsation of NPs leads to the generation of thermal waves, and the resulting attenuation is dependent on the difference in thermal properties between the continuous and dispersed phases. Overall, US attenuation, described by the attenuation coefficient, can be expressed as the sum of intrinsic absorption (the combination of ultrasonic attenuation in the NPs and the continuous medium), visco-inertial attenuation, thermal attenuation, and scattering losses 75 , 76 . US hyperthermia studies performed in tissue-mimicking phantoms indicate that US attenuation increases ~20-150%, resulting in a temperature increase of ~20-70%, when the phantoms are doped with scatterers 77 .…”
“…As ultrasonic waves propagate through most materials, this spectroscopy does not require optical transparency. Although ultrasonic spectroscopy has been utilised for material analysis for a long time and has demonstrated various successful applications (Povey and Mason, 1998;Povey, 1997;Holmes and Povey, 2017), the capability of this technique in analysis of molecular processes has been restricted by a number of factors. These include limited resolution and precision in measurements of ultrasonic parameters, the requirement for large sample volumes and often complicated measuring procedures.…”
Abstract. High-resolution ultrasonic spectroscopy (HR-US) is an analytical technique for direct and non-destructive monitoring of molecular and micro-structural transformations in liquids and semi-solid materials. It is based on precision measurements of ultrasonic velocity and attenuation in analysed samples. The application areas of HR-US in research, product development, and quality and process control include analysis of conformational transitions of polymers, ligand binding, molecular self-assembly and aggregation, crystallisation, gelation, characterisation of phase transitions and phase diagrams, and monitoring of chemical and biochemical reactions. The technique does not require optical markers or optical transparency. The HR-US measurements can be performed in small sample volumes (down to droplet size), over broad temperature range, at ambient and elevated pressures, and in various measuring regimes such as automatic temperature ramps, titrations and measurements in flow.
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