Ultrasonic enhancement of antibiotic action on several species of bacteria.

Rediske, Andrea M.; Hymas, Weston; Wilkinson, R.; Pitt, William G.

doi:10.2323/jgam.44.283

Cited by 50 publications

(41 citation statements)

References 13 publications

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“…Previously, our lab has reported the use of low-frequency ultrasound to enhance the activity of antibiotics against planktonic and sessile Gram-negative bacteria in vitro and in vivo (6,7,16,18,23,24). The combination of gentamicin and low-frequency ultrasound was very effective in reducing viability of E. coli biofilms.…”

Section: Discussionmentioning

confidence: 99%

“…Our previous research showed that ultrasound enhanced the activity of aminoglycosides against Gram-negative bacteria in both planktonic and biofilm phenotypes (6,(13)(14)(15)(16)(17)(18). Ultrasound also increased killing of Gram-positive S. epidermidis and Streptococcus mitis by ampicillin (16).…”

Section: Introductionmentioning

confidence: 99%

“…Ultrasound also increased killing of Gram-positive S. epidermidis and Streptococcus mitis by ampicillin (16). Insonation is hypothesized to increase the effectiveness of antibiotics by increasing the rate of antibiotic transport to the bacteria (19,20), by increasing the permeability of the cell membrane (21,22), and by increasing the metabolic activity and growth of the bacteria, perhaps by increasing oxygen and other nutrient transport (19).…”

Section: Introductionmentioning

confidence: 99%

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Ultrasonically Enhanced Vancomycin Activity Against Staphylococcus Epidermidis Biofilms in Vivo

et al. 2004

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SUMMARYInfection of implanted medical devices by Gram-positive organisms such as Staphylococcus ssp. is a serious concern in the biomaterial community. In this research the application of low frequency ultrasound to enhance the activity of vancomycin against implanted Staphylococcus epidermidis biofilms was examined. Polyethylene disks covered with a biofilm of S. epidermidis were implanted subcutaneously in rabbits on both sides of their spine. The rabbits received systemic vancomycin for the duration of the experiment. Following 24 h of recovery, one disk was insonated for 24 or 48 h while the other was a control. Disks were removed and viable bacteria counted. At 24 h of insonation, there was no difference in viable counts between control and insonated biofilms, while at 48 h of insonation there were statistically fewer viable bacteria in the insonated biofilm. The S. epidermidis biofilms responded favorably to combinations of ultrasound and vancomycin, but longer treatment times are required for this Gram-positive organism than was observed previously for a Gram-negative species.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ultrasonically Enhanced Vancomycin Activity Against Staphylococcus Epidermidis Biofilms in Vivo

et al. 2004

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“…Chemotherapy-Rediske et al showed that ultrasound increased the killing of bacteria both in planktonic suspension [196,197] and biofilm forms [198][199][200] in the presence of antibiotics. This synergistic killing effect was most pronounced at lower frequencies and decreased as the frequency of insonation increased [201,202].…”

Section: Antibacterialmentioning

confidence: 99%

Ultrasonic drug delivery – a general review

Pitt

Husseini

Staples

2004

Expert Opinion on Drug Delivery

530

428

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Ultrasound (US) has an ever-increasing role in the delivery of therapeutic agents including genetic material, proteins, and chemotherapeutic agents. Cavitating gas bodies such as microbubbles are the mediators through which the energy of relatively non-interactive pressure waves is concentrated to produce forces that permeabilize cell membranes and disrupt the vesicles that carry drugs. Thus the presence of microbubbles enormously enhances delivery of genetic material, proteins and smaller chemical agents. Delivery of genetic material is greatly enhanced by ultrasound in the presence of microbubbles. Attaching the DNA directly to the microbubbles or to gas-containing liposomes enhances gene uptake even further. US-enhanced gene delivery has been studied in various tissues including cardiac, vascular, skeletal muscle, tumor and even fetal tissue. US-enhanced delivery of proteins has found most application in transdermal delivery of insulin. Cavitation events reversibly disrupt the structure of the stratus corneum to allow transport of these large molecules. Other hormones and small proteins could also be delivered transdermally. Small chemotherapeutic molecules are delivered in research settings from micelles and liposomes exposed to ultrasound. Cavitation appears to play two roles: it disrupts the structure of the carrier vesicle and releases the drug; it also makes the cell membranes and capillaries more permeable to drugs. There remains a need to better understand the physics of cavitation of microbubbles and the impact that such cavitation has upon cells and drug-carrying vesicles.

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“…One of these biofilm-control signal analogues (the RNAIII-inhibiting peptide analogue of the RNAIII-activating peptide signal) has been shown to block biofilm formation by all species of Staphylococcus and thus facilitate the killing of cells of these species even in the presence of a biomaterial (34). Biofilm engineering has also contributed new technologies of potential interest in the control of biofilm infections, in that biofilms have been shown to be much more susceptible to conventional antibiotics in direct current electric fields (42) or when treated with ultrasonic radiation (43). As biofilm science and engineering continue their exponential growth and biofilm problems in industry are solved, these concepts and technologies will be made available to medicine as long as we hold fast to the biofilm concept of chronic infection.…”

Section: Figurementioning

confidence: 99%

The application of biofilm science to the study and control of chronic bacterial infections

Costerton¹,

Veeh²,

Shirtliff³

et al. 2007

J. Clin. Invest.

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Unequivocal direct observations have established that the bacteria that cause device-related and other chronic infections grow in matrix-enclosed biofilms. The diagnostic and therapeutic strategies that have served us so well in the partial eradication of acute epidemic bacterial diseases have not yielded accurate data or favorable outcomes when applied to these biofilm diseases. We discuss the potential benefits of the application of the new methods and concepts developed by biofilm science and engineering to the clinical management of infectious diseases.

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Ultrasonic enhancement of antibiotic action on several species of bacteria.

Cited by 50 publications

References 13 publications

Ultrasonically Enhanced Vancomycin Activity Against Staphylococcus Epidermidis Biofilms in Vivo

Ultrasonically Enhanced Vancomycin Activity Against Staphylococcus Epidermidis Biofilms in Vivo

Ultrasonic drug delivery – a general review

The application of biofilm science to the study and control of chronic bacterial infections

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