Ultrasonic delivery of a chemotherapeutic agent using acoustic droplet vaporization (ADV)

Fabiilli, Mario L.; Sebastian, Ian E.; Haworth, Kevin J.; Kripfgans, Oliver D.; Carson, Paul L.; Fowlkes, J. Brian

doi:10.1109/ultsym.2009.5442058

Cited by 3 publications

(3 citation statements)

References 5 publications

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“…This phenomenon, termed acoustic droplet vaporization (ADV) 2 , has been proposed a number of in vitro and in vivo applications such as vascular occlusion 3 , diagnostic imaging 4,5 , ultrasound-mediated tissue ablation 6 and drug delivery 7,8 . In vitro studies have shown that the smaller the droplet diameter, the higher the vaporization threshold and high acoustic pressures are required for vaporization 9,10 . Although a nanometer-scale droplet has advantages of stability at physiological temperature and efficient for extravasation into tissue 3,8 , it may require pressures higher than diagnostic ultrasound machines provide, increasing the potential for unwanted bioeffects 11 .…”

Section: Introductionmentioning

confidence: 99%

PLGA/PFC particles loaded with gold nanoparticles as dual contrast agents for photoacoustic and ultrasound imaging

et al. 2014

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Phase-change contrast agents consisting of a perfluorocarbon (PFC) liquid core stabilized by a lipid, protein, or polymer shell have been proposed for a variety of clinical applications. Previous work has demonstrated that vaporization can be induced by laser irradiation through optical absorbers incorporated inside the droplet. In this study, Poly-lactide-coglycolic acid (PLGA) particles loaded with PFC liquid and silica-coated gold nanoparticles (GNPs) were developed and characterized using photoacoustic (PA) methods. Microsized PLGA particles were loaded with PFC liquid and GNPs (14, 35, 55nm each with a 20nm silica shell) using a double emulsion method. The PA signal intensity and optical vaporization threshold were investigated using a 375 MHz transducer and a focused 532-nm laser (up to 450-nJ per pulse). The laser-induced vaporization threshold energy decreased with increasing GNP size. The vaporization threshold was 850, 690 and 420 mJ/cm 2 for 5µm-sized PLGA particles loaded with 14, 35 and 55 nm GNPs, respectively. The PA signal intensity increased as the laser fluence increased prior to the vaporization event. This trend was observed for all particles sizes. PLGA particles were then incubated with MDA-MB-231 breast cancer cells for 6 hours to investigate passive targeting, and the vaporization of the PLGA particles that were internalized within cells. The PLGA particles passively internalized by MDA cells were visualized via confocal fluorescence imaging. Upon PLGA particle vaporization, bubbles formed inside the cells resulting in cell destruction. This work demonstrates that GNPs-loaded PLGA/PFC particles have potential as PA theranostic agents in PA imaging and optically-triggered drug delivery systems.

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Section: Introductionmentioning

confidence: 99%

PLGA/PFC particles loaded with gold nanoparticles as dual contrast agents for photoacoustic and ultrasound imaging

et al. 2014

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“…Emulsions are drawing increased attention, and so-called phase-shift emulsions (generally containing low boiling point fluorocarbons) can give ultrasound-triggered release when emulsion droplets vaporize in response to insonation. 17 Ganta and Amiji 18 have used a combination of paclitaxel and curcumin in nanoemulsion formulations to overcome multidrug resistance in SKOV3 TR ovarian adenocarcinoma cells.…”

Section: Introductionmentioning

confidence: 99%

In vitro suppression of oral squamous cell carcinoma growth by ultrasound-mediated delivery of curcumin microemulsions

Lin

Thomas²,

Chen³

et al. 2012

IJN

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There is increasing interest in using natural products as anticancer agents, as many have antioxidative properties that may help to prevent cellular damage that can lead to cancer. In addition, there is the expectation that many natural products will have low toxicity and few side effects. However, most anticancer and antioxidative agents are hydrophobic, reducing their bioavailability in vivo and making them problematic to deliver. Curcumin provides a good model system for study. In low doses it shows both anticancer and antioxidation effects, whereas in high doses and delivered locally it could be cytotoxic for cancer cells. In this paper, curcumin microemulsions were formed with food-grade chemicals, including soybean lecithin, soybean oil, and Tween 80, a Food and Drug Administration-approved surfactant. The optimized composition formed curcumin microemulsions with a mean size of 40–50 nm, carrying a concentration of curcumin as high as 15 μM. The stability of curcumin microemulsions refrigerated at 5°C over at least 968 days was assessed by size distribution and zeta potential. The effects of low-frequency ultrasound on two oral squamous cell carcinoma cell lines (OSCC-4 and OSCC-25), and the synergy between treatment with curcumin microemulsions and low-frequency sonic stimulation, were tested. Finally, microscopic imaging of the cells confirmed the toxic effects of the curcumin microemulsions, showing damaged and ruptured cells after treatment. Brief exposure to the curcumin-containing microemulsions did have cytotoxic effects, but the addition of ultrasound strongly enhanced those effects, especially on OSCC-25 cells.

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“…Incorporation of poly(ethylene glycol)-containing surfactants appears to enhance the sensitivity of lipid-based carriers to sound,16 possibly by interfering with the “healing” of membrane defects. Emulsions are drawing increased attention, and so-called phase-shift emulsions (generally containing low boiling point fluorocarbons) can give ultrasound-triggered release when emulsion droplets vaporize in response to insonation 17. Ganta and Amiji18 have used a combination of paclitaxel and curcumin in nanoemulsion formulations to overcome multidrug resistance in SKOV3 TR ovarian adenocarcinoma cells.…”

Section: Introductionmentioning

confidence: 99%

Nanotherapeutics: Emerging Competent Technology in Neuroaids and CNS Drug Delivery

2012

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There is increasing interest in using natural products as anticancer agents, as many have antioxidative properties that may help to prevent cellular damage that can lead to cancer. In addition, there is the expectation that many natural products will have low toxicity and few side effects. However, most anticancer and antioxidative agents are hydrophobic, reducing their bioavailability in vivo and making them problematic to deliver. Curcumin provides a good model system for study. In low doses it shows both anticancer and antioxidation effects, whereas in high doses and delivered locally it could be cytotoxic for cancer cells. In this paper, curcumin microemulsions were formed with food-grade chemicals, including soybean lecithin, soybean oil, and Tween 80, a Food and Drug Administration-approved surfactant. The optimized composition formed curcumin microemulsions with a mean size of 40-50 nm, carrying a concentration of curcumin as high as 15 μM. The stability of curcumin microemulsions refrigerated at 5°C over at least 968 days was assessed by size distribution and zeta potential. The effects of low-frequency ultrasound on two oral squamous cell carcinoma cell lines (OSCC-4 and OSCC-25), and the synergy between treatment with curcumin microemulsions and low-frequency sonic stimulation, were tested. Finally, microscopic imaging of the cells confirmed the toxic effects of the curcumin microemulsions, showing damaged and ruptured cells after treatment. Brief exposure to the curcumin-containing microemulsions did have cytotoxic effects, but the addition of ultrasound strongly enhanced those effects, especially on OSCC-25 cells.

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Ultrasonic delivery of a chemotherapeutic agent using acoustic droplet vaporization (ADV)

Cited by 3 publications

References 5 publications

PLGA/PFC particles loaded with gold nanoparticles as dual contrast agents for photoacoustic and ultrasound imaging

PLGA/PFC particles loaded with gold nanoparticles as dual contrast agents for photoacoustic and ultrasound imaging

In vitro suppression of oral squamous cell carcinoma growth by ultrasound-mediated delivery of curcumin microemulsions

Nanotherapeutics: Emerging Competent Technology in Neuroaids and CNS Drug Delivery

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