Ultrasonic-assisted synthesis of amino-functionalized Fe 3 O 4 @SiO 2 magnetic β- cyclodextrin nanocarrier for controlled release of doxorubicin anticancer drug

Abstract

β-cyclodextrin (β-CD) as cyclic oligosaccharide can be widely employed for preparation of controlled delivery of drugs. Hence, we synthesized a novel β-CD-based nanocarrier with superparamagnetic and molecular recognition properties for controlled release of doxorubicin (DOX) drug. The nanocarrier was prepared by using a facile ultrasonic-assisted method and through surface reversible addition fragmentation chain transfer (RAFT) copolymerization of core-shell Fe3O4@SiO2 nanoparticles onto vinylated β-CD. The spherical amino-functionalized Fe3O4@SiO2 nanomaterials were synthesized easily by a co-condensation green process of Fe3O4 nanoparticles with tetraethyl orthosilicate (TEOS) and (3-Aminopropyl) trimethoxysilane (APTES). The structure and composition of the as-prepared drug delivery nanocomposites were studied by FTIR, SEM, TEM, XRD, and VSM. Then, the loading and releasing behaviors of DOX drug have been investigated in detail. First of all, the loading of DOX was confirmed by UV-Vis spectra and SEM and TEM images of drug-loaded samples. The results also exhibited that the drug loading efficiency is found to be dependent on initial drug concentration, β-CD content and temperature. The drug loading capacity of nanocarrier was also compared with pure β-CD. Moreover, it was revealed that in vitro release rate of DOX was dependent on physiological solution pH. Finally, the drug release kinetics data were well fitted to the Higuchi model. In addition, biological characterizations were tested by MTT assay, which approved the high performance of the nanocarriers in killing cancerous cells. Due to its unique and distinct advantageous and properties, the synthesized nanocarriers can be utilized as a potential and promising biodegradable drug carrier for controlled and sustained release of various drugs.