Ultrasonic-assisted extraction for flavonoid compounds content and antioxidant activities of India Moringa oleifera L. leaves: Simultaneous optimization, HPLC characterization and comparison with other methods

Lin, Xue; Wu, Lingfeng; Xiong, Weihao; Yao, Linling; Wang, Lu

doi:10.1016/j.jarmap.2020.100284

Cited by 55 publications

(36 citation statements)

References 52 publications

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“…The calibration curve of gallic acid (Y = 0.0032 X – 0.0004, R 2 = 0.996) was drawn. Total flavonoid content (TFC) was tested with the aluminum chloride method ( 18 , 19 ). Let 100 μl of extract react with 50 μl of 5% NaNO 2 ( w/v ) for 5 min and then, add 50 μl of 10% AlCl 3 ( w/v ) for another 6 min of reaction.…”

Section: Methodsmentioning

confidence: 99%

LC-Q-Orbitrap-MS/MS Characterization, Antioxidant Activity, and α-Glucosidase-Inhibiting Activity With In Silico Analysis of Extract From Clausena Indica (Datz.) Oliv Fruit Pericarps

Wang

et al. 2021

Front. Nutr.

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Clausena indica (Datz.) Oliv fruit pericarps (CIOPs) is an important agro-industrial by-product rich in active components. In this article, the effects of traditional and green deep eutectic solvents (DESs) on the high-performance liquid chromatography (HPLC) characterization, antioxidant activities, and α-glucosidase-inhibitory activity of phenolic extracts from CIOPs were investigated for the first time. The results showed that ChCl-Gly and Bet-CA had higher extraction efficiency for the total phenolic content (TPC, 64.14–64.83 mg GAE/g DW) and total flavonoid content (TFC, 47.83–48.11 mg RE/g DW) compared with the traditional solvents (water, methanol, and ethyl acetate). LC-Q-Orbitrap-MS/MS was adopted to identify the phenolic compositions of the CIOPs extracts. HPLC-diode array detection (HPLC-DAD) results indicated that arbutin, (–)-epigallocatechin, chlorogenic acid, procyanidin B1, (+)-catechin, and (–)-epicatechin were the major components for all extracts, especially for deep eutectic solvents (DESs). In addition, ChCl-Xyl and ChCl-Gly extracts showed higher antioxidant activities against 2,2-diphenyl-1-picrylhydrazyl (DPPH•), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS+•), ferric reducing antioxidant power (FRAP), reducing power (RP), and cupric ion reducing antioxidant capacity (CUPRAC) than extracts extracted by other solvents. A strong α-glucosidase-inhibiting activity (IC50, 156.25-291.11 μg/ml) was found in three DESs extracts. Furthermore, in silico analysis of the major phenolics in the CIOPs extracts was carried out to explore their interactions with α-glucosidase. Multivariate analysis was carried out to determine the key factors affecting the antioxidant activity and α-glucosidase-inhibiting activity. In short, DES can be taken as a promising solvent for valorization and recovery of bioactive compounds from agro-industrial by-products. The results verified that CIOPs can be used as a prospective source rich in bio-active compounds applied in the food and pharmacy industries.

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Section: Methodsmentioning

confidence: 99%

LC-Q-Orbitrap-MS/MS Characterization, Antioxidant Activity, and α-Glucosidase-Inhibiting Activity With In Silico Analysis of Extract From Clausena Indica (Datz.) Oliv Fruit Pericarps

Wang

et al. 2021

Front. Nutr.

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“…TPC was expressed as mg gallic acid equivalent (GAE)/g dry weight (DW). TFC was measured by the aluminum chloride colorimetric method proposed by Wang et al [22]. The absorbance was read at 510 nm and compared to a rutin calibration curve (Y = 0.0005X − 0.0007, R 2 = 0.9978).…”

Section: Preparation and Chemical Characteristics Analysis Of Dessmentioning

confidence: 99%

Customized Deep Eutectic Solvents as Green Extractants for Ultrasonic-Assisted Enhanced Extraction of Phenolic Antioxidants from Dogbane Leaf-Tea

Wang

Zhang

et al. 2021

Foods

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This study evaluates the application of eco-friendly deep eutectic solvents (DESs) in the extraction of phenolic antioxidants from dogbane leaf-tea (DLT). The results showed DESs with lower viscosity allowed an efficient extraction of significantly higher contents of total phenolics or flavonoids. An innovative and high-efficient solvent, choline chloride-levulinic acid (ChCl-LevA), was screened and used in ultrasonic-assisted extraction (UAE) of phenolic compounds from DLT. According to full factorial design experimental results, total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity, and anti-α-glucosidase activity (α-GIA) of the DLT extracts were simultaneously optimized by response surface methodology. Sonication temperature and water content in ChCl-LevA were found to be the major factors affecting the TPC, TFC, antioxidant activity, and α-GIA of DLT extracts. Under the optimum parameters (water content in ChCl-LevA was 45%, sonication temperature was 50 °C, and extraction time was 30 min), the measured results for all the responses were obtained as follows: TPC-91.38 ± 7.20 mg GAE/g DW, TFC-84.12 ± 3.47 mg RE/g DW, ABTS+-492 ± 7.33 mmol TE/g DW, FRAP-6235 ± 121 μmol Fe(II)/g DW and α-GIA-230 ± 7.59 mmol AE/g DW, which were consistent with the predicted values. In addition, strongly significant positive correlations were observed between TPC/TFC and bio-activities of the DLT extracts. HPLC results indicated high contents of (-)-epigallocatechin (4272 ± 84.86 μg/g DW), catechin (5268 ± 24.53 μg/g DW), isoquercitrin (3500 ± 86.07 μg/g DW), kaempferol 3-O-rutinoside (3717 ± 97.71 μg/g DW), and protocatechuic acid (644 ± 1.65 μg/g DW) were observed in the DLT extracts. In contrast to other extraction methods, ChCl-LevA-based UAE yielded higher TPC, TFC, individual phenolic contents, stronger antioxidant activity, and α-GIA. Scanning electron microscope (SEM) analysis further confirmed that ChCl-LevA-based UAE enhanced the disruption of cell wall structure, thereby making more phenolic antioxidants released from DLT. In short, ChCl-LevA-based UAE was confirmed to be an innovative and high-efficient method for extraction of phenolic antioxidants from DLT. Dogbane leaves can be considered as a good tea source rich in natural antioxidants.

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“…The mechanism of extraction by ultrasound consists in disintegrating plant cells and releasing their contents into the extraction solvent [29]. The most important parameters of ultrasound-assisted extraction include: time and temperature of process, liquid-to-solid ratio, type, concentration and pH of the organic solvent, and elements related to the operation of the ultrasound system itself, such as: ultrasound intensity and frequency, system type (ultrasonic bath or ultrasonic probe) and ultrasound mode (continuous or pulsed) [30][31][32][33].…”

Section: Introductionmentioning

confidence: 99%

Process Efficiency and Energy Consumption during the Ultrasound-Assisted Extraction of Bioactive Substances from Hawthorn Berries

et al. 2021

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This study investigated the impact of sonication parameters on the efficiency of the extraction of bioactive substances from hawthorn berries. The ultrasonic treatment was performed in two modes: continuous and pulse. In the pulse mode, the samples were sonicated with the following processor settings: 1 s on-2 s off. The effective ultrasonic processor times were 5, 10, and 15 min, and the total extraction times were 15 min, 30 min, and 45 min. The content of total polyphenols and total anthocyanins was determined by a spectrophotometric method. We show that the operating mode of the processor affects extraction efficiency, energy consumption and unit energy inputs. Extraction supported by a pulsating ultrasonic field allowed saving from 20% to 51% of energy with a simultaneous higher efficiency of the process. In addition, we show that the unit energy consumption in the pulsed mode was about 40% to 68% lower than the energy consumption in the case of continuous operation.

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Ultrasonic-assisted extraction for flavonoid compounds content and antioxidant activities of India Moringa oleifera L. leaves: Simultaneous optimization, HPLC characterization and comparison with other methods

Cited by 55 publications

References 52 publications

LC-Q-Orbitrap-MS/MS Characterization, Antioxidant Activity, and α-Glucosidase-Inhibiting Activity With In Silico Analysis of Extract From Clausena Indica (Datz.) Oliv Fruit Pericarps

LC-Q-Orbitrap-MS/MS Characterization, Antioxidant Activity, and α-Glucosidase-Inhibiting Activity With In Silico Analysis of Extract From Clausena Indica (Datz.) Oliv Fruit Pericarps

Customized Deep Eutectic Solvents as Green Extractants for Ultrasonic-Assisted Enhanced Extraction of Phenolic Antioxidants from Dogbane Leaf-Tea

Process Efficiency and Energy Consumption during the Ultrasound-Assisted Extraction of Bioactive Substances from Hawthorn Berries

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