“… 37 Our findings are analogous to those previously reported by. 45 Figure 6 In vitro anticancer activity of KF-CeO 2 NPs against the HeLa carcinoma cells in terms of cell viability percentage compared to CH-CeO 2 NPs and Kochiae Fructus extract (**** p < 0.0001). …”
Introduction
Kochiae Fructus has been widely used in Chinese Herbal medicine to treat various diseases. We report a rapid and eco-friendly approach for cerium oxide (CeO
2
) nanoparticles (NPs) synthesis using the extract of medicinally important plant “Kochiae Fructus”, and the synthesized NPs were named KF-CeO
2
NPs.
Methods
Various spectroscopic approaches such as transmission electron microscope (TEM), powder X-ray diffraction (XRD), and energy-dispersive X-Ray (EDX) were used to characterize the KF-CeO
2
NPs effectively. The antibacterial and biofilm inhibition activity of KF-CeO
2
NPs against Gram-positive and Gram-negative multi-drug resistant (MDR) bacteria was determined using the serial dilution method and XTT assay. KF-CeO
2
NPs were assessed for anticancer activity against HeLa cancer cells using an MTT assay. Cytobiocompatibility was determined in two normal cell lines (3T3 and hMSC).
Results and Discussion
The average size of the KF-CeO
2
NPs was 11.3 ± 3.9 nm with spherical morphology. KF-CeO
2
NPs demonstrated a greater than 95% bactericidal efficacy against MDR microorganisms. In addition, KF-CeO
2
NPs strongly suppressed (more than 79%) the biofilms of MDR bacteria, indicating their potential for addressing antibiotic resistance issues. Compared to Kochiae Fructus extract and CH-CeO
2
NPs, they exhibited significant cytotoxic effects (35.60% cell viability) on HeLa cancer cells. In addition, the KF-CeO
2
NPs were shown to be highly biocompatible with hMSC and 3T3 cell lines (85.13% and 81.17% cell viability, respectively), suggesting that they may be employed in biological systems.
Conclusion
These data indicate that KF-CeO
2
NPs synthesized using Kochiae Fructus extract are promising alternative treatments for MDR. In addition, this study will give the potential for the sustained development of biocompatible NPs with enhanced biological capabilities derived from vital pharmaceutical plants.
“… 37 Our findings are analogous to those previously reported by. 45 Figure 6 In vitro anticancer activity of KF-CeO 2 NPs against the HeLa carcinoma cells in terms of cell viability percentage compared to CH-CeO 2 NPs and Kochiae Fructus extract (**** p < 0.0001). …”
Introduction
Kochiae Fructus has been widely used in Chinese Herbal medicine to treat various diseases. We report a rapid and eco-friendly approach for cerium oxide (CeO
2
) nanoparticles (NPs) synthesis using the extract of medicinally important plant “Kochiae Fructus”, and the synthesized NPs were named KF-CeO
2
NPs.
Methods
Various spectroscopic approaches such as transmission electron microscope (TEM), powder X-ray diffraction (XRD), and energy-dispersive X-Ray (EDX) were used to characterize the KF-CeO
2
NPs effectively. The antibacterial and biofilm inhibition activity of KF-CeO
2
NPs against Gram-positive and Gram-negative multi-drug resistant (MDR) bacteria was determined using the serial dilution method and XTT assay. KF-CeO
2
NPs were assessed for anticancer activity against HeLa cancer cells using an MTT assay. Cytobiocompatibility was determined in two normal cell lines (3T3 and hMSC).
Results and Discussion
The average size of the KF-CeO
2
NPs was 11.3 ± 3.9 nm with spherical morphology. KF-CeO
2
NPs demonstrated a greater than 95% bactericidal efficacy against MDR microorganisms. In addition, KF-CeO
2
NPs strongly suppressed (more than 79%) the biofilms of MDR bacteria, indicating their potential for addressing antibiotic resistance issues. Compared to Kochiae Fructus extract and CH-CeO
2
NPs, they exhibited significant cytotoxic effects (35.60% cell viability) on HeLa cancer cells. In addition, the KF-CeO
2
NPs were shown to be highly biocompatible with hMSC and 3T3 cell lines (85.13% and 81.17% cell viability, respectively), suggesting that they may be employed in biological systems.
Conclusion
These data indicate that KF-CeO
2
NPs synthesized using Kochiae Fructus extract are promising alternative treatments for MDR. In addition, this study will give the potential for the sustained development of biocompatible NPs with enhanced biological capabilities derived from vital pharmaceutical plants.
“…Finally, the obtained product was washed thrice with H 2 O (distilled water) and dried at 100°C for 12 h to get CdO NPs/c‐MWCNT nanocomposite fibres. The method for obtaining the nanostructure‐based system has been reported earlier [17].…”
Section: Methodsmentioning
confidence: 99%
“…A standard MTT assay was used to evaluate the number of healthy cells after treating them with CdO NPs, c‐MWCNT NPs and CdO NPs/c‐MWCNT nanocomposite. The cell viability calculation based on the MTT assay has been reported earlier [17]. HeLa cells in the concentration of 1 × 106 cells/ml were taken into 96 well plates.…”
“…(7.8, 15.6, 31.2, 62.5, 125, 250, 500, and 1000 μg/mL) of CeO2/GO nanohybrid for 24, 48, and 72 h. They found a significant dose-and timedependent decrease in the cell viability with IC 50 values of 250, 125, and 31.2 μg/mL for 24, 48, and 72 h, respectively. Moreover, maximum HeLa cells were found to be arrested at G2/M phase (Saranya et al 2020).…”
Cancer is one of the leading causes of death throughout the world. Various conventional therapeutic techniques, such as surgery, chemotherapy, radiation therapy, immunotherapy, and hormone therapy, have been implemented to cure
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