Ultrasoft edge-labelled hydrogel sensors reveal internal tissue stress patterns in invasive engineered tumors

Lee, Chang Kyu; Boghdady, Christina-Marie; Lelarge, Virginie; Leask, Richard L.; McCaffrey, Luke; Moraes, Christopher

doi:10.1016/j.biomaterials.2023.122073

Cited by 4 publications

(5 citation statements)

References 38 publications

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“…To address this issue, Lee and colleagues loaded brightly stable fluorescent nanoparticles onto the surface of hydrogel microspheres and created edge‐marked MSG (eMSG). [ 38 ] Subsequent experimental results confirmed that the eMSG with nano‐micron combined structures could generate clearly defined discrete fluorescent signals and significantly improve the signal‐to‐noise ratio in determining MSG deformation. This enables the generation of tissue internal stress and its monitoring over time, and provides an objective and convenient strategy for understanding tissue stress changes at the cellular level.…”

Section: Current Biomedical Applicationsmentioning

confidence: 85%

“…Clear definition of discrete fluorescence signals. [38] Significant improvement in signal-to-noise ratio.…”

Section: Biosensorsmentioning

confidence: 99%

“…[35][36][37] Furthermore, with the development of nanoscience and technology, the application of hydrogel microspheres with "nano-micron combination" structures has rapidly expanded into various other fields, including rapid biochemical detection, medical imaging, biosensors, and microrobotics, and is becoming a frontier and hotspot of research in numerous domains. [38][39][40][41] Therefore, to aid researchers in swiftly grasping the current research status and accelerating the future development of related fields, it is necessary to timely summarize the advanced hydrogel microspheres with "nano-micron combination" structures. However, a comprehensive summary of these advanced hydrogel microspheres is currently lacking, including the types and functions of nanomaterials, materials and preparation methods of hydrogel microspheres, and strategies for loading nanomaterials into hydrogel microspheres.…”

Section: Introductionmentioning

confidence: 99%

“…[ 35–37 ] Furthermore, with the development of nanoscience and technology, the application of hydrogel microspheres with “nano‐micron combination” structures has rapidly expanded into various other fields, including rapid biochemical detection, medical imaging, biosensors, and microrobotics, and is becoming a frontier and hotspot of research in numerous domains. [ 38–41 ]…”

Section: Introductionmentioning

confidence: 99%

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Nano‐Micron Combined Hydrogel Microspheres: Novel Answer for Minimal Invasive Biomedical Applications

Liu,

Du,

Chen

et al. 2024

Macromol. Rapid Commun.

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Hydrogels, key in biomedical research for their hydrophilicity and versatility, have evolved with hydrogel microspheres (HMs) of micron‐scale dimensions, enhancing their role in minimally invasive therapeutic delivery, tissue repair, and regeneration. The recent emergence of nanomaterials has ushered in a revolutionary transformation in the biomedical field, which demonstrates tremendous potential in targeted therapies, biological imaging, and disease diagnostics. Consequently, the integration of advanced nanotechnology promises to trigger a new revolution in the realm of hydrogels. HMs loaded with nanomaterials combine the advantages of both hydrogels and nanomaterials, which enables multifaceted functionalities such as efficient drug delivery, sustained release, targeted therapy, biological lubrication, biochemical detection, medical imaging, biosensing monitoring, and micro‐robotics. Here, this review comprehensively expounds upon commonly used nanomaterials and their classifications. Then, it provides comprehensive insights into the raw materials and preparation methods of HMs. Besides, the common strategies employed to achieve nano‐micron combinations are summarized, and the latest applications of these advanced nano‐micron combined HMs in the biomedical field are elucidated. Finally, valuable insights into the future design and development of nano‐micron combined HMs are provided.

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Section: Current Biomedical Applicationsmentioning

confidence: 85%

“…Clear definition of discrete fluorescence signals. [38] Significant improvement in signal-to-noise ratio.…”

Section: Biosensorsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Nano‐Micron Combined Hydrogel Microspheres: Novel Answer for Minimal Invasive Biomedical Applications

Liu,

Du,

Chen

et al. 2024

Macromol. Rapid Commun.

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show abstract

“…Mechanical forces arising from budding morphogenesis have been established to play an important role in directing cell function in the gut 23 and lungs 24,25 for example; and are known to affect fusion in systems such as muscle 26 and bone 27 . Such stress patterns can spontaneously arise during 3D tissue morphogenesis [28][29][30] to directly impact cell function 31 . In placental models specifically, endogenous mechanical stress patterns have been computationally predicted to arise from trophoblast proliferation and fusion 32 , and such endogenous stresses have been experimentally shown to affect trophoblast fusion via models that tune substrate stiffness and colony sizes [33][34][35] .…”

Section: Introductionmentioning

confidence: 99%

Directed biomechanical compressive forces enhance fusion efficiency in model placental trophoblast cultures

Parameshwar,

Li,

Arnauts

et al. 2024

Preprint

Self Cite

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The syncytiotrophoblast is a multinucleated structure that arises from fusion of mononucleated cytotrophoblasts, to sheath the placental villi and regulate transport across the maternal-fetal interface. Here, we ask whether the dynamic mechanical forces that must arise during villous development might influence fusion, and explore this question using in vitro choriocarcinoma trophoblast models. We demonstrate that mechanical stress patterns arise around sites of localized fusion in cell monolayers, in patterns that match computational predictions of villous morphogenesis. We then externally apply these mechanical stress patterns to cell monolayers and demonstrate that equibiaxial compressive stresses (but not uniaxial or equibiaxial tensile stresses) enhance expression of the syndecan-1 marker of fusion. These findings suggest that the mechanical stresses that contribute towards sculpting the placental villi may also impact fusion in the developing tissue. We then extend this concept towards 3D cultures and demonstrate that fusion can be enhanced by applying low isometric compressive stresses to spheroid models, even in the absence of an inducing agent. These results indicate that mechanical stimulation is a potent activator of cellular fusion, suggesting novel avenues to improve experimental reproductive modelling, placental tissue engineering, and understanding disorders of pregnancy development.

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Recent Advances in Hydrogel-Based Biosensors for Cancer Detection

Sun,

Chen

2024

ACS Appl. Mater. Interfaces

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Early cancer detection is crucial for effective treatment, but current methods have limitations. Novel biomaterials, such as hydrogels, offer promising alternatives for developing biosensors for cancer detection. Hydrogels are threedimensional and cross-linked networks of hydrophilic polymers that have properties similar to biological tissues. They can be combined with various biosensors to achieve high sensitivity, specificity, and stability. This review summarizes the recent advances in hydrogel-based biosensors for cancer detection, their synthesis, their applications, and their challenges. It also discusses the implications and future directions of this emerging field.

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Ultrasoft edge-labelled hydrogel sensors reveal internal tissue stress patterns in invasive engineered tumors

Cited by 4 publications

References 38 publications

Nano‐Micron Combined Hydrogel Microspheres: Novel Answer for Minimal Invasive Biomedical Applications

Nano‐Micron Combined Hydrogel Microspheres: Novel Answer for Minimal Invasive Biomedical Applications

Directed biomechanical compressive forces enhance fusion efficiency in model placental trophoblast cultures

Recent Advances in Hydrogel-Based Biosensors for Cancer Detection

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