Ultrasmall compact CMOS imaging system for bioluminescence reporter-based live gene expression analysis

Olorocisimo, Joshua Philippe; Briones, Jeric; Sasagawa, Kiyotaka; Haruta, Makito; Takehara, Hironari; Tashiro, Hiroyuki; Ishida-Kitagawa, Norihiro; Bessho, Yasumasa; Ohta, Jun

doi:10.1117/1.jbo.26.11.116002

Cited by 5 publications

(3 citation statements)

References 35 publications

(51 reference statements)

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“…Moreover, this will be helpful for the design of microdevices that excel in biocompatibility and do not interfere with plant growth, as such devices could be placed on or inside plants based on an accurate distance between the sensor and plant tissues. This means that existing fluorescence and luminescence imaging devices used for animals and cultured cells 8–10 could be adapted for plants.…”

Section: Resultsmentioning

confidence: 99%

Monitoring plant growth by analyzing their morphology using microfocus X‐ray CT

Kunieda,

Hayashi,

Kumagai

et al. 2024

Elect Comm in Japan

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The growth and development of embryophytes is deeply influenced by environmental stimuli, such as light, temperature, and soil nutrients. Understanding the mechanisms underlying the growth response of plants to environmental stimuli is crucial for agriculture. In this study, we examined the morphology of a flowering plant, Arabidopsis thaliana, using microfocus X‐ray computed tomography (µCT), which enables non‐destructive analysis of the external and internal structures of plants. Three‐dimensional (3D) images of the plant, which were reconstructed from X‐ray scanned data, clearly showed the shapes of its leaves, stems, and buds from any angle. At a higher magnification, the µCT also revealed the small hair‐like structures called trichomes on the Arabidopsis leaf epidermis. However, motion artifacts found in the 3D‐reconstructed images indicated that plant's growth rate was faster than scanning speed. Thus, scan parameters must be accordingly optimized. Additionally, CT‐based 3D printing can be used to design micro devices that can be further used to monitor plant growth. These results suggest that µCT is a useful technique for analyzing morphology of growing plants.

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Section: Resultsmentioning

confidence: 99%

Monitoring plant growth by analyzing their morphology using microfocus X‐ray CT

Kunieda,

Hayashi,

Kumagai

et al. 2024

Elect Comm in Japan

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“…The size of these forms is affected by light scattering, but the contribution of this has been experimentally determined [ 29 ]. Increasing the sensitivity and resolution of the imaging device can also be further improved by using hybrid optical filters [ 30 , 31 ], narrow-band light sources [ 32 , 33 ], and improved pixel design [ 34 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

Multi-Region Microdialysis Imaging Platform Revealed Dorsal Raphe Nucleus Calcium Signaling and Serotonin Dynamics during Nociceptive Pain

Akbar

Castillo

Olorocisimo

et al. 2023

IJMS

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In this research, we combined our ultralight micro-imaging device for calcium imaging with microdialysis to simultaneously visualize neural activity in the dorsal raphe nucleus (DRN) and measure serotonin release in the central nucleus of the amygdala (CeA) and the anterior cingulate cortex (ACC). Using this platform, we observed brain activity following nociception induced by formalin injection in the mouse’s hind paw. Our device showed that DRN fluorescence intensity increased after formalin injection, and the increase was highly correlated with the elevation in serotonin release in both the CeA and ACC. The increase in calcium fluorescence intensity occurred during the acute and inflammatory phases, which suggests the biphasic response of nociceptive pain. Furthermore, we found that the increase in fluorescence intensity was positively correlated with mouse licking behavior. Lastly, we compared the laterality of pain stimulation and found that DRN fluorescence activity was higher for contralateral stimulation. Microdialysis showed that CeA serotonin concentration increased only after contralateral stimulation, while ACC serotonin release responded bilaterally. In conclusion, our study not only revealed the inter-regional serotonergic connection among the DRN, the CeA, and the ACC, but also demonstrated that our device is feasible for multi-site implantation in conjunction with a microdialysis system, allowing the simultaneous multi-modal observation of different regions in the brain.

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“…Due to the variable baseline and dynamic range of the imaging device, it can cause sudden offsets and instability in the data. Thus, further improvements to the signal-to-noise ratio of the imaging device can be made by using an upgraded pixel architecture [35].…”

Section: Application In Seizure Monitoringmentioning

confidence: 99%

Brain-implantable needle-type CMOS imaging device enables multi-layer dissection of seizure calcium dynamics in the hippocampus

Olorocisimo,

Ohta,

Regonia

et al. 2024

J. Neural Eng.

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Current neuronal imaging methods use bulky lenses that either impede animal behavior or prohibit multi-depth imaging. To overcome these limitations, we developed a lightweight lensless biophotonic system for neuronal imaging, enabling compact and simultaneous visualization of multiple brain layers. Our developed "CIS-NAIST" device integrates a micro-CMOS image sensor, thin-film fluorescence filter, micro-LEDs, and a needle-shaped flexible printed circuit. With this device, we monitored neuronal calcium dynamics during seizures across the different layers of the hippocampus. The CIS-NAIST device revealed distinct calcium activity patterns across the CA1, molecular interlayer, and dentate gyrus. Our findings indicated an elevated calcium amplitude activity specifically in the dentate gyrus compared to other layers. Then, leveraging the multi-layer data obtained from the device, we employed machine learning techniques for seizure classification and prediction. Using Long-Short TermMemory and Hidden Markov Models, we successfully classified seizure calcium activity and predicted seizure behavior based on the multi-layer imaging data. Taken together, our device can enable a minimally invasive method of seizure monitoring that can help elucidate the mechanisms of temporal lobe epilepsy.

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Ultrasmall compact CMOS imaging system for bioluminescence reporter-based live gene expression analysis

Cited by 5 publications

References 35 publications

Monitoring plant growth by analyzing their morphology using microfocus X‐ray CT

Monitoring plant growth by analyzing their morphology using microfocus X‐ray CT

Multi-Region Microdialysis Imaging Platform Revealed Dorsal Raphe Nucleus Calcium Signaling and Serotonin Dynamics during Nociceptive Pain

Brain-implantable needle-type CMOS imaging device enables multi-layer dissection of seizure calcium dynamics in the hippocampus

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