Ultraslow microdialysis and microfiltration for in-line, on-line and off-line monitoring

Korf, Jakob; Huinink, Kirsten D.; Posthuma-Trumpie, Geertruida A.

doi:10.1016/j.tibtech.2009.12.005

Cited by 29 publications

(22 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While efforts are being made to improve secretome studies in vivo [3,20] several in vitro studies have generated valuable data that can be interrogated in vivo using human tissue biopsies and/or human body fluids [3]. However, one of the challenges in in vitro secretome studies is discriminating truly cell released proteins from proteins that are released due to cell death or leakage during culturing.…”

Section: Discussionmentioning

confidence: 99%

The human secretome atlas initiative: Implications in health and disease conditions

Brown

Seol

Pillai

et al. 2013

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

View full text Add to dashboard Cite

Proteomic analysis of human body fluids is highly challenging, therefore many researchers are redirecting efforts towards secretome profiling. The goal is to define potential biomarkers and therapeutic targets in the secretome that can be traced back in accessible human body fluids. However, currently there is a lack of secretome profiles of normal human primary cells making it difficult to assess the biological meaning of current findings. In this study we sought to establish secretome profiles of human primary cells obtained from healthy donors with the goal of building a human secretome atlas. Such an atlas can be used as a reference for discovery of potential disease associated biomarkers and eventually novel therapeutic targets. As a preliminary study, secretome profiles were established for six different types of human primary cell cultures and checked for overlaps with the three major human body fluids including plasma, cerebrospinal fluid and urine. About 67% of the 1054 identified proteins in the secretome of these primary cells occurred in at least one body fluid. Furthermore, comparison of the secretome profiles of two human glioblastoma cell lines to this new human secretome atlas enabled unambiguous identification of potential brain tumor biomarkers. These biomarkers can be easily monitored in different body fluids using stable isotope labeled standard proteins. The long term goal of this study is to establish a comprehensive online human secretome atlas for future use as a reference for any disease related secretome study.

show abstract

Section: Discussionmentioning

confidence: 99%

The human secretome atlas initiative: Implications in health and disease conditions

Brown

Seol

Pillai

et al. 2013

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

View full text Add to dashboard Cite

show abstract

“…3 Schematic illustration of the processes of microdialysis and microfiltration a function of the materials membranes are made from (borosilicate, silicon carbide, quartz/teflon, ceramic, polyethersulfone, regenerated cellulose) and varies among molecules (e.g. as a function of charge or hydrophilicity) (Guggenberger and Zech 1992;Murphy et al 2000;Wessel-Bothe et al 2000;Huinink et al 2010;Korf et al 2010). Hence, absolute quantification requires testing of recovery of target molecules.…”

Section: Sampling Of Organic N From Soil Solutionmentioning

confidence: 99%

Organic N molecules in the soil solution: what is known, what is unknown and the path forwards

Warren

2013

Plant Soil

View full text Add to dashboard Cite

Background Many biological questions about N availability and the N cycle require knowledge of the abundance and identity of molecules comprising the pool of organic N. Moreover, basic knowledge of the molecular composition of the soil solution can give rise to new hypotheses via data-driven or inductive reasoning. Scope This paper examines the composition of organic N molecules in the soil solution. Our perception of organic N in the soil solution is shaped by analytical approaches, and thus I briefly review approaches for sampling and analysis of the soil solution. I give examples of hypotheses generated by knowledge of the molecular composition of organic N and conclude by suggesting priorities for future research. Conclusions Studies of the molecular composition of organic N are very much in their infancy. Amino acids, their oligomers and polymers are consistently large components of the pool of organic N. The soil solution also contains organic N compounds from at least another 12 compound classes, but almost nothing is known about their functional significance. Uncovering the role of these other compounds in the N cycle would enrich our understanding of organic N and the N cycle, and place studies of amino acids and their polymers in a broader context.

show abstract

“…1 Micro-dialysis has often been used in the clinical environment for continuously harvesting body fluids in a minimal invasive manner, but a drawback of this process are variable recovery rates. 2,3 Some general considerations in microdialysis have been reviewed also by Nandi and Lunte. 4 For intensive care units, different approaches have been chosen, using either subcutaneously implanted microdialysis catheters, intravascularly inserted probes or extracorporeal microdialysis with continuously sampled and heparinised whole blood, from which dialysates have been obtained.…”

Section: Introductionmentioning

confidence: 99%

Ex-vivo glucose sensors using micro-dialysis: importance of on-line recovery rate determination by multi-analyte infrared spectrometry

et al. 2015

View full text Add to dashboard Cite

Micro-dialysis has been established in the clinical environment for continuously harvesting body fluids, but a drawback of this process are variable recovery rates, which can be observed especially for subcutaneously implanted catheters. Perfusates with either acetate or mannitol have been investigated as recovery markers. The latter substance is suggested for application with external cavity tuneable quantum cascade lasers, rendering a limited wavenumber interval in contrast to FTIR-spectrometers. Despite the overlap of mannitol and glucose spectra, their simultaneous quantification was successful. By investigating the depletion of the marker substances from the perfusates using different micro-dialysis devices, the theoretical nonlinear relationship between the relative dialysate marker concentration and glucose recovery rate was confirmed for the marker substance-analyte pair of acetate and glucose, rendering a basis for reliable blood glucose measurements. For the pair of mannitol and glucose an almost linear dependency was expected for the microdialysate catheters and experimentally verified, which provides a straightforward correction of any dialysis recovery rate variation during patient monitoring.

show abstract

Ultraslow microdialysis and microfiltration for in-line, on-line and off-line monitoring

Cited by 29 publications

References 70 publications

The human secretome atlas initiative: Implications in health and disease conditions

The human secretome atlas initiative: Implications in health and disease conditions

Organic N molecules in the soil solution: what is known, what is unknown and the path forwards

Ex-vivo glucose sensors using micro-dialysis: importance of on-line recovery rate determination by multi-analyte infrared spectrometry

Contact Info

Product

Resources

About