Ultrashort ssDNA in Retinoblastoma Patients Blood Plasma Detected by a Novel High Resolution HPLC Technique: a Preliminary Report

Abstract

A B ST R AC T A significant population of ultrashort (50-150n) single-stranded DNA fragments were found in exosome-free blood plasma of retinoblastoma patients (6.84 ng mL −1 ), but not in plasma of healthy donors. An original high resolution HPLC technique has been proposed to reveal and characterize this peculiarity. To solve this task, a novel molecular size exclusion -anion exchange analytical technique was developed. Its applicability to diagnostics and oncogenesis research is quizzed here. K E Y WO R D S Retinoblastoma; blood plasma; cfDNA; ssDNA; HPLC

Exosomes derived from stem cells as an emerging therapeutic strategy for intervertebral disc degeneration

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Mesenchymal Stem Cell–Derived Extracellular Vesicles Protect Rat Nucleus Pulposus Cells from Oxidative Stress

“…EVs typically range from 30 to 1,000 nm in diameter. 2 They are released in biological fluids, including blood, 3,4 amniotic fluid, 5 synovial fluid, 6 saliva, 7 and other body fluids. 8 The functional mechanism of EVs is accomplished via body fluids.…”

The potential role of stem cells‐derived extracellular vesicles in the treatment of musculoskeletal system diseases

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