Ultrashort Open‐Tubular Capillary Column with Modified Silica‐Gel Thin Layer for Capillary Electrochromatography

Tsuda, Toshitaka; Matsuki, Shiro; Munesue, Motonori; Kitagawa, Shinya; Ochi, Hiizu

doi:10.1081/jlc-120018415

Cited by 6 publications

(8 citation statements)

References 20 publications

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“…This revival of CEC is because it is a microcolumn electroseparation technique that combines the best features of high-performance liquid chromatography (HPLC) with those of high-performance capillary electrophoresis (HPCE). In essence, CEC mainly couples the high selectivity of HPLC and the high separation efficiency of HPCE [9][10][11][12][13][14][15]. This is because in CEC, the mobile phase solvent is transported through a capillary by the use of electroosmotic flow (EOF), which is induced by applying an electrical field across the capillary column.…”

Section: Introductionmentioning

confidence: 99%

“…Several options have been proposed to increase the surface area, and therefore, to increase the interactions between the analyte and the coated phase. These options include polymer coatings, porous silica layers, etching, and sol-gel techniques [11,30,78,83].…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, preparation of the column, and particularly the stationary phase packed or immobilized on the inner walls of the capillary, is critical for CEC. The capillary columns in CEC are usually classified into three main formats [10,11,17,[26][27][28][29][30][31][32][33]: (i) packed-CEC [12][13][14][15][16][34][35][36][37][38][39][40][41], (ii) open-tubular CEC (OT-CEC) [28][29][30][31][32][42][43][44][45][46][47][48][49][50][51][52][53], and (iii) monolithic (or continuous rod) CEC [9,27,.…”

Section: Introductionmentioning

confidence: 99%

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Analytical separations in open‐tubular capillary electrochromatography

Kapnissi‐Christodoulou

Zhu

Warner

2003

Electrophoresis

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This review represents a summary of recent progress in open-tubular capillary electrochromatography (OT-CEC) for chiral and achiral separations. The OT-CEC approach is an alternative to packed-CEC that could eliminate the problems associated with retaining frits and silica particles. In OT-CEC, the stationary phase is immobilized on the inner walls of the capillary. Preparation of the stationary phase is critical for OT-CEC. The preparation methods for capillary columns include (i) adsorption, (ii) covalent bonding and/or cross-linking, (iii) porous layers, (iv) chemical bonding after etching, (v) sol-gel, and (vi) molecular imprinting. Major developments, potential applications, technical difficulties and advantages associated with these wall coatings in OT-CEC are presented. In addition, the coupling of OT-CEC with mass spectrometry (MS) is briefly reviewed. Several applications of this hyphenated technique for analytical separations are also summarized.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Analytical separations in open‐tubular capillary electrochromatography

Kapnissi‐Christodoulou

Zhu

Warner

2003

Electrophoresis

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“…Recently, Tsuda et al [170] described a new method for the preparation of a thin silica gel layer on the inner wall of an open tubular capillary, by polymerizing silica oligomers followed by alkyl silanes modification. Only 2 cm long columns, modified with docosyl methyl dichlorsilane, were used for the separation of a-chymotrypsin and lysozyme.…”

Section: Open Tubular Capillariesmentioning

confidence: 99%

Recent progress in high‐performance capillary bioseparations

et al. 2003

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Bioanalysis is a fast growing domain with new technological developments in the field of mass spectrometry, separation science, and bioinformatics. The central role of separation prior to detection and evaluation of analytes is indisputable. In fact, since the decipherment of the human genome via multichannel capillary electrophoresis in combination with laser-induced fluorescence [1], the analysis of the proteome, i.e., the type and amount of all proteins expressed at a certain time by the genome in a cell or tissue [2] became of central interest. Therefore, new fast and sensitive capillary liquid chromatographic, electrophoretic, and electrochromatographic separation techniques, suitable for high-throughput analysis of proteins, peptides and nucleic acids, are required.

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“…11 In our previous study, we developed a preparation method for an OTCC by using silica oligomer to form a thin porous layer on the inner wall. 12 Our column was prepared with multiple steps in the fabrication of a thin porous layer and its silanization. Both processes are important to achieve good separation efficiency.…”

mentioning

confidence: 99%