Ultrasensitive therapeutic drug monitoring of methotrexate by a structure-switching aptamer with cascade primer exchange reaction

He, Junqing; Wang, Junyan; Zhang, Min; Shi, Guoyue

doi:10.1039/d2an01658c

Cited by 5 publications

(1 citation statement)

References 18 publications

(21 reference statements)

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“…In general, PER can generate ssDNA of a defined sequence and be used for signal amplification in immunofluorescence and RNA-FISH. [32,33] In 2019, Kishi et al reported a method of introducing the signal amplification by exchange reaction (SABER) to fluorescence in situ hybridization (FISH) resulting in the enhancement of FISH signal strength. [34] The target ssDNA synthesized using PER laid the foundation for the SABER technique.…”

Section: F I G U R Ementioning

confidence: 99%

Recent advances in the synthesis of single‐stranded DNA in vitro

Li,

Tan,

Jia

et al. 2024

Biotechnology Journal

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Single‐stranded DNA (ssDNA) is the foundation of modern biology, with wide applications in gene editing, sequencing, DNA information storage, and materials science. However, synthesizing ssDNA with high efficiency, high throughput, and low error rate in vitro remains a major challenge. Various methods have been developed for ssDNA synthesis, and some significant results have been achieved. In this review, six main methods were introduced, including solid‐phase oligonucleotide synthesis, terminal deoxynucleotidyl transferase‐based ssDNA synthesis, reverse transcription, primer exchange reaction, asymmetric polymerase chain reaction, and rolling circle amplification. The advantages and limitations of each method were compared, as well as illustrate their representative achievements and applications. Especially, rolling circle amplification has received significant attention, including ssDNA synthesis, assembly, and application based on recent work. Finally, the future challenges and opportunities of ssDNA synthesis were summarized and discussed. Envisioning the development of new methods and significant progress will be made in the near future with the efforts of scientists around the world.

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Section: F I G U R Ementioning

confidence: 99%