Ultrasensitive Semiautomated Chemiluminescent Immunoassay for Estradiol

England, Barry G.; Parsons, George H.; Possley, R. M.; McConnell, Daniel S.; Midgley, A. Rees

doi:10.1093/clinchem/48.9.1584

Cited by 53 publications

(15 citation statements)

References 3 publications

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“…Animals were assigned to the following 5 groups: 1) OIL (0.1 ml peanut oil vehicle); 2) E (1 μg, 2 μg or 5 μg 17β-estradiol in 0.1 ml peanut oil; there were no differences among the groups and so data from these animals were combined). In an independent study, serum estradiol concentrations were determined from trunk blood 1 hr after hormone injection according to a previously published method [29]; pg/ml ± SEM were -1 μg: 245±52, 2 μg: 408±59, 5 μg: 1146±108 (n=4-5/group); 3) P (125 μg or 500 μg progesterone, progesterone doses based on [12,42]; there was no effect of dose of P, so data from these groups were combined); 4) 5 μg E with 125 μg or 500 μg P given concurrently (EPC) and 5) 5 μg E with P sequential (5 μg E for 3 days, 125 μg or 500 μg P on day 4, oil on day 5; EPS). As illustrated in Figure 1, animals were placed into the rotometers to habituate for 30 minutes (Habituation), then they received hormone or oil treatment according to their group assignment (Hormone/Oil), thirty minutes later (the time when we see behavioral and neurochemical effects of estradiol [e.g., 10,12]) half of the rats in each group received cocaine (10 mg/ kg) and the other half received saline as a control (Cocaine/Saline).…”

Section: Experiments 1 Sensitization Of Cocaine-induced Rotational Bementioning

confidence: 99%

Interactions among ovarian hormones and time of testing on behavioral sensitization and cocaine self-administration

Yang

Zhao

et al. 2007

Behavioural Brain Research

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Ovarian hormones play a role in the use of drugs of abuse in women. In female rats estradiol has been shown to enhance acquisition of cocaine self-administration and behavioral sensitization induced by repeated cocaine treatment. Experiments were conducted to determine the effects of estradiol and/or progesterone on cocaine self-administration and behavioral sensitization to cocaine (10mg/kg; in animals with unilateral 6-hydroxydopamine lesions). Five groups of ovariectomized females were tested: (1) oil vehicle; (2) estradiol (E); (3) progesterone (P); (4) estradiol and progesterone given concurrently (EPC); (5) estradiol and progesterone given sequentially (EPS: 3 days of estradiol, 1 day progesterone, 1 day oil). All animals were tested during the dark phase of the light:dark cycle at ZT1600 and ZT2000-2100. Behavioral sensitization results: there was substantial conditioned turning throughout the habituation periods, and all animals exhibited behavioral sensitization with repeated cocaine treatment. Multivariate analysis indicated a significant effect of hormone treatment, time of day and day of testing. When individual groups were compared, however, only at ZT1600 did the E-treated and the EPS-treated animals show a trend (p<0.06) for greater behavioral sensitization to cocaine relative to the oil-treated animals. Self-administration results: all groups showed rapid acquisition of cocaine self-administration at 0.3 mg/kg/infusion, so we did not see an effect of ovarian hormones on acquisition, or a difference between groups tested at ZT1600 versus ZT2100 (p<0.05). There was, however, enhanced total intake of cocaine at 0.75 mg/kg/infusion in the E and the EPS groups. Concurrent administration of progesterone with estradiol counteracted the effect of estradiol on cocaine intake at 0.75 mg/kg/infusion, while progesterone alone did not enhance cocaine self-administration.

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Section: Experiments 1 Sensitization Of Cocaine-induced Rotational Bementioning

confidence: 99%

Interactions among ovarian hormones and time of testing on behavioral sensitization and cocaine self-administration

Yang

Zhao

et al. 2007

Behavioural Brain Research

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“…Such methods of analysis require the preparation of a volatile estradiol derivative. Immunoassay techniques can also be used for analysis of serum estradiol levels [3,[21][22][23]. In these procedures, an antibody to estradiol must be prepared.…”

Section: Discussionmentioning

confidence: 99%

Estradiol Solubility in Aqueous Systems: Effect of Ionic Concentrations, pH, and Organic Solvents

Carter

Sluss

2013

Journal of Hormones

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This study examined the effects of ionic strengths of NaCl (0.1, 0.3, and 1.0 M), pH (3, 7, and 11), and organic solvents (dichloromethane, diethyl ether, and methanol) on the extraction of estradiol at concentrations of 5.0 pg/mL in human serum. Methanol extracted almost 100% of the estradiol at a 5.0 pg/mL concentration, while ether and dichloromethane extracted only 73% or 70%, respectively, of the estradiol. The methanol extracted material was subjected to reverse phase high-performance liquid chromatography (HPLC) using 60% methanol and was found to elute at the same position as estradiol standard. These results suggest that methanol extraction of estradiol may prove useful in situations where estradiol occurs at concentration levels of ≥5.0 pg/mL, concentrations of great clinical significance in the detection and treatment of breast cancer.

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“…The lower limit of detection was 1 pg/ml. Assay cross‐reactivity was 0·75% for estrone, 0·28% for estriol and 0·00% for norethinedrone 22 …”

Section: Methods and Proceduresmentioning

confidence: 99%

Genetic polymorphisms and obesity influence estradiol decline during the menopause

Sowers

Randolph

Zheng

et al. 2011

Clinical Endocrinology

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Summary Objectives Obesity and genetic variation in aromatase and type 1 17-β hydroxysteroid dehydrogenase (HSD) could influence the E2 trajectory of decline during the menopause transition. Design and participants E2 trajectories during the menopause transition (phenotype) were identified using 5934 data points acquired annually from 681 women in Study of Women’s Health across the Nation (SWAN), a multiethnic study of the mid-life. E2 trajectories were related to CYP19 and type I 17-βHSD single-nucleotide polymorphisms (SNPs) and obesity. Results logE2 trajectories began to decline precipitously 2 years before the final menstrual period (FMP). The trajectory of the logE2 decline varied with genotypes and obesity. logE2 rates of decline were greater in nonobese women than in obese women, P < 0.05. Women with the CYP19rs936306 CT variant had logE2 rate of decline that was 54% as rapid as the rate of decline of women with the TT variant, P < 0.05. logE2 rate of decline in women with the CYP19rs749292 GG variant was two-thirds the rate of logE2 decline in women with the AG variant, P < 0.05. logRates of E2 decline with 17-βHSD SNPs (rs2830, rs592389, and rs615942) varied according to genotype within obesity groups. Within each obesity group, logE2 rate of decline was greater in heterozygous variants and much less in homozygotes (P < 0.05). Obese women with selected CYP19 and 17-β HSD gene variants had remarkably different E2 trajectories around the FMP, resulting in different postmenopausal E2 levels. The rate of the E2 decline and the subsequent postmenopausal E2 levels may be relevant to oestrogen-sensitive chronic diseases including cancers.

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Ultrasensitive Semiautomated Chemiluminescent Immunoassay for Estradiol

Cited by 53 publications

References 3 publications

Interactions among ovarian hormones and time of testing on behavioral sensitization and cocaine self-administration

Interactions among ovarian hormones and time of testing on behavioral sensitization and cocaine self-administration

Estradiol Solubility in Aqueous Systems: Effect of Ionic Concentrations, pH, and Organic Solvents

Genetic polymorphisms and obesity influence estradiol decline during the menopause

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