Ultrasensitive sandwich-type immunosensor for cardiac troponin I based on enhanced electrocatalytic reduction of H<sub>2</sub>O<sub>2</sub> using β-cyclodextrins functionalized 3D porous graphene-supported Pd@Au nanocubes

Zhang, Xiaobo; Lv, Hui; Li, Yueyun; Zhang, Chunyan; Wang, Ping; Liu, Qing; Ai, Bing; Xu, Zhen; Zhao, Zengdian

doi:10.1039/c8tb03362e

Cited by 29 publications

(14 citation statements)

References 45 publications

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“… 29 Similar research was reported by Zhao et al., who synthesized Pd@Au nanocube-doped three-dimensional porous graphene to establish an ultrasensitive electrochemical immunosensor for detecting cTnI. 45 This nanostructure displayed good capture capability targeting antibodies and accelerated the electron transfer process on the electrode surface. Another method to increase of detection sensitivity is to modify nanomaterials with more signal transducer elements.…”

Section: Nanotechnology Approaches To Detect Cadssupporting

confidence: 60%

Nanotechnology for cardiovascular diseases

Fang

et al. 2022

The Innovation

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Section: Nanotechnology Approaches To Detect Cadssupporting

confidence: 60%

Nanotechnology for cardiovascular diseases

Fang

et al. 2022

The Innovation

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“…Last, of particular note is work by Zhang and co-workers [ 172 ] who reported a complex electrode configuration but yet provided the basis of a sensor for the ultrasensitive determination of cTnI with a LOD of 33.3 fg mL −1 and a linear range from 1 × 10 −4 to 100 ng mL −1 . The sensor is a sandwich type sensor based upon the use of nanoparticles labelled with antibodies with the sensing mechanism occurring via a “signal on” approach.…”

Section: Current Electrochemical/electroanalytical Approaches To Dete...mentioning

confidence: 99%

Electroanalytical point-of-care detection of gold standard and emerging cardiac biomarkers for stratification and monitoring in intensive care medicine - a review

et al. 2022

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Determination of specific cardiac biomarkers (CBs) during the diagnosis and management of adverse cardiovascular events such as acute myocardial infarction (AMI) has become commonplace in emergency department (ED), cardiology and many other ward settings. Cardiac troponins (cTnT and cTnI) and natriuretic peptides (BNP and NT-pro-BNP) are the preferred biomarkers in clinical practice for the diagnostic workup of AMI, acute coronary syndrome (ACS) and other types of myocardial ischaemia and heart failure (HF), while the roles and possible clinical applications of several other potential biomarkers continue to be evaluated and are the subject of several comprehensive reviews. The requirement for rapid, repeated testing of a small number of CBs in ED and cardiology patients has led to the development of point-of-care (PoC) technology to circumvent the need for remote and lengthy testing procedures in the hospital pathology laboratories. Electroanalytical sensing platforms have the potential to meet these requirements. This review aims firstly to reflect on the potential benefits of rapid CB testing in critically ill patients, a very distinct cohort of patients with deranged baseline levels of CBs. We summarise their source and clinical relevance and are the first to report the required analytical ranges for such technology to be of value in this patient cohort. Secondly, we review the current electrochemical approaches, including its sub-variants such as photoelectrochemical and electrochemiluminescence, for the determination of important CBs highlighting the various strategies used, namely the use of micro- and nanomaterials, to maximise the sensitivities and selectivities of such approaches. Finally, we consider the challenges that must be overcome to allow for the commercialisation of this technology and transition into intensive care medicine. Graphical abstract

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“…The authors detected cTnI in a linear detection range of 0.01-10 ng/mL with high reproducibility (Sandil et al, 2019). Zhang et al (2019) synthesized β-cyclodextrins-functionalized (CDs) 3D porous graphene-supported Pd@Au nanocubes (NCs) for cTnI detection. CDs were able to increase the dispersibility of the 3D-porous graphene and improve the capability of Ab 2 capture.…”

Section: Electrical and Reductionmentioning

confidence: 99%

“…The transfer was based on the reduction of H 2 O 2 on the Th-modified electrode surface, which further amplified the signal response. The authors detected cTnI with a low detection limit of 33.3 fg/mL through the EC signal amplification labeling in the sandwich format (Zhang et al, 2019).…”

Section: Electrical and Reductionmentioning

confidence: 99%

Nanoscale Technologies in Highly Sensitive Diagnosis of Cardiovascular Diseases

Shi

Yang

et al. 2020

Front. Bioeng. Biotechnol.

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Cardiovascular diseases (CVD) are the leading cause of death and morbidity in the world and are a major contributor to healthcare costs. Although enormous progress has been made in diagnosing CVD, there is an urgent need for more efficient early detection and the development of novel diagnostic tools. Currently, CVD diagnosis relies primarily on clinical symptoms based on molecular imaging (MOI) or biomarkers associated with CVDs. However, sensitivity, specificity, and accuracy of the assay are still challenging for early-stage CVDs. Nanomaterial platform has been identified as a promising candidate for improving the practical usage of diagnostic tools because of their unique physicochemical properties. In this review article, we introduced cardiac biomarkers and imaging techniques that are currently used for CVD diagnosis. We presented the applications of various nanotechnologies on diagnosis within cardiac immunoassays (CIAs) and molecular imaging. We also summarized and compared different cardiac immunoassays based on their sensitivities and working ranges of biomarkers.

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Ultrasensitive sandwich-type immunosensor for cardiac troponin I based on enhanced electrocatalytic reduction of H₂O₂ using β-cyclodextrins functionalized 3D porous graphene-supported Pd@Au nanocubes

Abstract: A signal amplification principle based on increased electrocatalytic reduction of H2O2 by the CDs-3D-PG-Pd@Au NCs using the mediated effect of Th.

Cited by 29 publications

References 45 publications

Nanotechnology for cardiovascular diseases

Nanotechnology for cardiovascular diseases

Electroanalytical point-of-care detection of gold standard and emerging cardiac biomarkers for stratification and monitoring in intensive care medicine - a review

Nanoscale Technologies in Highly Sensitive Diagnosis of Cardiovascular Diseases

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Ultrasensitive sandwich-type immunosensor for cardiac troponin I based on enhanced electrocatalytic reduction of H2O2 using β-cyclodextrins functionalized 3D porous graphene-supported Pd@Au nanocubes

Abstract: A signal amplification principle based on increased electrocatalytic reduction of H2O2 by the CDs-3D-PG-Pd@Au NCs using the mediated effect of Th.

Cited by 29 publications

References 45 publications

Nanotechnology for cardiovascular diseases

Nanotechnology for cardiovascular diseases

Electroanalytical point-of-care detection of gold standard and emerging cardiac biomarkers for stratification and monitoring in intensive care medicine - a review

Nanoscale Technologies in Highly Sensitive Diagnosis of Cardiovascular Diseases

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Ultrasensitive sandwich-type immunosensor for cardiac troponin I based on enhanced electrocatalytic reduction of H₂O₂ using β-cyclodextrins functionalized 3D porous graphene-supported Pd@Au nanocubes