Ultrasensitive ratiometric fluorescent pH and temperature probes constructed from dye-labeled thermoresponsive double hydrophilic block copolymers

Hu, Jinming; Zhang, Xiaozheng; Wang, Di; Hu, Xianglong; Liu, Tao; Zhang, Guoying; Liu, Shiyong

doi:10.1039/c1jm13575a

Cited by 76 publications

(60 citation statements)

References 69 publications

(81 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Signal fluctuations can result from variations in local probe concentration, sample thickness, pH, or temperature, which make subsequent interpretation of single-intensity data complicated. Ratiometric FRETbased sensors for the analysis of nucleoside polyphosphates, pH, temperature, hydrogen peroxide (H 2 O 2 ), mercury (Hg), and chromium (Cr) ions have all been demonstrated [79, [86][87][88][89][90].…”

Section: Environmentally Sensitive Fluorophoresmentioning

confidence: 99%

Materials for FRET Analysis: Beyond Traditional Dye–Dye Combinations

Sapsford

Wildt

Mariani

et al. 2013

FRET – Förster Resonance Energy Transfer

View full text Add to dashboard Cite

The intrinsic sensitivity (r 6 dependence) of F€ orster (or fluorescence) resonance energy transfer (FRET) to nanoscale changes in the donor/acceptor separation distance has made FRET an invaluable biophysical tool in a variety of applications, ranging from studying the structure and conformation of proteins and nucleic acids to examining biomolecular interactions, including its use in in vitro and in vivo bioassays [1][2][3][4][5][6][7]. While the myriad of FRET configurations and techniques currently in use are covered throughout this book, here we focus primarily on the materials utilized as donor or acceptor probes in FRET rather than the process itself [3,5,8,9]. Our 2006 review paper on this topic serves as the foundation for this updated chapter [3]. The materials were divided into three main categories: organic materials that include "traditional" dye fluorophores, dark quenchers, polymers, and carbon nanomaterials (NMs); inorganic materials such as metal chelates, metal, and semiconductor nanocrystals; and fluorophores of biological origin such as fluorescent proteins (FPs), amino acids, and fluorescence generated from enzymatic bioluminescence (BL) and chemiluminescence (CL). These materials may function as FRET donors and/or acceptors, depending upon experimental design. Many of the new materials developed and/or new donor-acceptor probe combinations used address some of the inherent complications of more traditional FRET materials, including photobleaching, spectral cross talk, and direct excitation of the acceptor species, and examples of these will be highlighted and discussed throughout the chapter. Since the vast majority of FRET applications are biological in nature, they routinely involve some type of biomolecule labeling strategy, which ultimately plays a significant and fundamental role in the success and interpretation of the resulting FRET. Therefore, we begin the chapter with a brief discussion of the bioconjugation techniques commonly utilized for FRET and points to consider, followed by sections highlighting the current and emerging materials that are used, or have the potential to be used, in FRET applications.

show abstract

Section: Environmentally Sensitive Fluorophoresmentioning

confidence: 99%

Materials for FRET Analysis: Beyond Traditional Dye–Dye Combinations

Sapsford

Wildt

Mariani

et al. 2013

FRET – Förster Resonance Energy Transfer

View full text Add to dashboard Cite

show abstract

“…By contrast, polymer-based fluorescent sensors can solve these problems; meanwhile, it is also considered as the fluorescent probe from experiment to practical application of an effective way. In recent years, polymer-based fluorescent sensors have been developed for measuring pH and temperature, and for detecting anions, cations and molecules [30][31][32][33][34].…”

Section: Introductionmentioning

confidence: 99%

Rhodamine derivative functionalized chitosan as efficient sensor and adsorbent for mercury(II) detection and removal

Shi

Yan

Wang

et al. 2015

Materials Research Bulletin

View full text Add to dashboard Cite

“…The chemosensors exhibited on/off fluorescence emission toward mercury ion (Hg 2+ ) chelation in an organic solution with a short response time, low background interference, low detection limits, long wavelength emission, and high selectivity [7]. Recent studies have demonstrated the high potential of rhodamine B (RhB)-copolymer-based chemosensors for temperature, pH, and certain HTM cations sensing in aqueous solutions because of their water solubility, simplicity, ability to change color, and high selectivity [9][10][11][12][13][14][15]. Liu et al reported…”

Section: Introductionmentioning

confidence: 99%

Novel Multifunctional Luminescent Electrospun Fluorescent Nanofiber Chemosensors-Filter and Their Versatile Sensing of pH, Temperature, and Metal Ions

Chen¹,

Lung²,

Kuo³

et al. 2018

Preprint

View full text Add to dashboard Cite

Novel multifunctional fluorescent chemosensors composed of electrospun (ES) nanofibers with high sensitivity toward pH, mercury ions (Hg2+), and temperature were prepared from poly(N-Isopropylacrylamide-co-N-methylolacrylamide-co-rhodamine derivative) (poly(NIPAAm-co-NMA-co-RhBN2AM)) by employing electrospinning process. NIPAAm and NMA moieties provide hydrophilic and thermo-responsive properties (absorption of Hg2+ in aqueous solutions), and chemical cross-linking sites (stabilization of the fibrous structure in aqueous solutions), respectively. The fluorescent probe, RhBN2AM is highly sensitive toward pH and Hg2+. Synthesis of poly(NIPAAm-co-NMA-co-RhBN2AM) with different compositions were carried on via free-radical polymerization. ES nanofibers prepared from sensory copolymers with a 71.1:28.4:0.5 NIPAAm: NMA: RhBN2AM ratio (P3 ES nanofibers) exhibited significant color change from nonfluorescent to red fluorescence while sensing pH or Hg2+, and high reversibility of on/off switchable fluorescence emission when Hg2+ and ethylenediaminetetraacetic acid (EDTA) were sequentially added. The P3 ES nanofibrous membranes had a higher surface-to-volume ratio to enhance their performance than did the corresponding thin films. In addition, the fluorescence emission of P3 ES nanofibrous membranes exhibited second enhancement above the lower critical solution temperature. Thus, the ES nanofibrous membranes prepared from P3 with on/off switchable capacity and thermo-responsive characteristics can be used as multifunctional sensory devise for specific heavy transition metal (HTM) in aqueous solutions.

show abstract

Ultrasensitive ratiometric fluorescent pH and temperature probes constructed from dye-labeled thermoresponsive double hydrophilic block copolymers

Cited by 76 publications

References 69 publications

Materials for FRET Analysis: Beyond Traditional Dye–Dye Combinations

Materials for FRET Analysis: Beyond Traditional Dye–Dye Combinations

Rhodamine derivative functionalized chitosan as efficient sensor and adsorbent for mercury(II) detection and removal

Novel Multifunctional Luminescent Electrospun Fluorescent Nanofiber Chemosensors-Filter and Their Versatile Sensing of pH, Temperature, and Metal Ions

Contact Info

Product

Resources

About