Ultrasensitive nanowire-based detection of HCVcoreAg in the serum using a microwave generator

Malsagova, Kristina A.; Pleshakova, Tatyana O.; Galiullin, Rafael A.; Kaysheva, Anna L.; Shumov, Ivan D.; Il’nitskii, M. A.; Popov, V.; Glukhov, Alexander V.; Archakov, Alexander I.; Ivanov, Yuri D.

doi:10.1039/c8ay00495a

Cited by 14 publications

(36 citation statements)

References 37 publications

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“…The design and characteristics of the SOI-NW structures used as sensor elements are described in [5,6,8]. SOI-NW structures with n-type conductance were used.…”

Section: Methodsmentioning

confidence: 99%

“…In the experiments, the width of the sensor elements was 3 nm, the thickness was 32 nm, the length was 10 nm, and the number of SOI-NWs on the chip was 12. The SOI-NW biosensor scheme is described in [5,8].…”

Section: Methodsmentioning

confidence: 99%

“…The surfaces of chips (either freshly prepared or stored for one or two years) were treated according to the standard procedure using isopropanol, water, and the solution of HF in CH 3 OH to remove organic contaminants by sequential double treatment [5,6,8]. The freshly prepared chips were then additionally placed into the ozonizer to form hydroxyl groups on the surface of the SOI-NW structures [5].…”

Section: Methodsmentioning

confidence: 99%

“…The freshly prepared chips were then additionally placed into the ozonizer to form hydroxyl groups on the surface of the SOI-NW structures [5]. The chips’ surfaces were silanized in APTES vapors according to the protocol described elsewhere [5,8].…”

Section: Methodsmentioning

confidence: 99%

“…SOI nanowire structures are formed with the use of complementary metal-oxide-semiconductor (CMOS)-compatible technology by gas phase reduction and lithography [5]. This biosensor has been found to be an efficient tool for the detection of proteins and nucleic acids with extremely high sensitivity at levels from 10 −17 to 10 −14 M [6,7,8].…”

Section: Introductionmentioning

confidence: 99%

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Detection of Marker miRNAs, Associated with Prostate Cancer, in Plasma Using SOI-NW Biosensor in Direct and Inversion Modes

Ivanov

Pleshakova

Malsagova

et al. 2019

Sensors

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Information about the characteristics of measuring chips according to their storage conditions is of great importance for clinical diagnosis. In our present work, we have studied the capability of chips to detect nanowire biosensors when they are either freshly prepared or have been stored for either one or two years in a clean room. Potential to detect DNA oligonucleotides (oDNAs)—synthetic analogues of microRNAs (miRNAs) 198 and 429 that are associated with the development of prostate cancer (PCa)—in buffer solution was demonstrated using a nanowire biosensor based on silicon-on-insulator structures (SOI-NW biosensor). To provide biospecific detection, nanowire surfaces were sensitized with oligonucleotide probes (oDNA probes) complimentary to the known sequences of miRNA 183 and 484. In this study it is demonstrated that freshly prepared SOI-NW biosensor chips with n-type conductance and immobilized oDNA probes exhibit responses to the addition of complimentary oDNAs in buffer, leading to decreases in chips’ conductance at a concentration of 3.3 × 10−16 M. The influence of storage time on the characteristics of SOI-NW biosensor chips is also studied herein. It is shown that a two-year storage of the chips leads to significant changes in their characteristics, resulting in “inverse” sensitivity toward negatively charged oDNA probes (i.e., through an increase in chips’ conductance). It is concluded that the surface layer makes the main contribution to conductance of the biosensor chip. Our results indicate that the detection of target nucleic acid molecules can be carried out with high sensitivity using sensor chips after long-term storage, but that changes in their surface properties, which lead to inversed detection signals, must be taken into account. Examples of the applications of such chips for the detection of cancer-associated microRNAs in plasma samples of patients with diagnosed prostate cancer are given. The results obtained herein are useful for the development of highly sensitive nanowire-based diagnostic systems for the revelation of (prostate) cancer-associated microRNAs in human plasma.

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“…The design and characteristics of the SOI-NW structures used as sensor elements are described in [5,6,8]. SOI-NW structures with n-type conductance were used.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%