Ultrasensitive Microfluidic Paper-Based Electrochemical Biosensor Based on Molecularly Imprinted Film and Boronate Affinity Sandwich Assay for Glycoprotein Detection

Abstract

In this work, we proposed a strategy that combined molecularly imprinted polymers (MIPs) and hybridization chain reaction into microfluidic paper-based analytical devices for ultrasensitive detection of target glycoprotein ovalbumin (OVA). During the fabrication, Au nanorods with a large surface area and superior conductibility were grown on paper cellulosic fiber as a matrix to introduce a boronate affinity sandwich assay. The composite of MIPs including 4-mercaptophenylboronic acid (MPBA) was able to capture target glycoprotein OVA. SiO2@Au nanocomposites labeled MPBA and cerium dioxide (CeO2)-modified nicked DNA double-strand polymers (SiO2@Au/dsDNA/CeO2) as a signal tag were captured into the surface of the electrode in the presence of OVA. An electrochemical signal was generated by using nanoceria as redox-active catalytic amplifiers in the presence of 1-naphthol in electrochemical assays. As a result, the electrochemical assay was fabricated and could be applied in the detection of OVA in the wide linear range of 1 pg/mL to 1000 ng/mL with a relatively low detection limit of 0.87 pg/mL (S/N = 3). The results indicated that the proposed platform possessed potential applications in clinical diagnosis and other related fields.

CeO2 Nanoparticle-Containing Polymers for Biomedical Applications: A Review

“…Another example in this field is provided by Sun et al [124], who studied an electrochemical biosensor based on a molecularly imprinted polymer (MIP) film for glycoprotein ovalbumin detection. In this biosensor, nanoceria was used as a redox active catalytic amplifier.…”

Nucleic Acids Analysis

“…With the development of nucleic acid technology, a broad spectrum of nucleic acid-based PADs has been exploited to detect a wide variety of analysts by electrochemical, colorimetric, chemiluminescent or fluorimetric readouts. Electrochemical PAD biosensors are constructed often through wax-printing or screen printing [378,379,[389][390][391]. In 2009, the first electrochemical PAD was developed by Dungchai et al [392].…”

“…The sensing interfaces are composed of oligonucleotide monolayers [379,412,420], composite multilayer [378,409] and 3D nucleic acid materials [399,410] or oligonucleotide-containing heterogeneous nanostructures [123]. The analysts include small molecules [375,421], miRNAs [378], DNA strands [123,379,412,420], proteins [375,379,391,422], exosomes [409], cancer cells [422], papillomavirus [389], pathogens [408] and so on, implying the great potential of nucleic acid-based PADs in enormous applications.…”

Paper and Other Fibrous Materials—A Complete Platform for Biosensing Applications

“…Some authors have reported using these techniques to deposit conductive inks to manufacture electrodes with biosensing applications. The most commonly used are made of carbon [ 88 , 104 ], silver chloride [ 100 , 104 ] and indium tin oxide (ITO) [ 204 ].…”