Ultrasensitive label-free electrochemical immunosensor of NT-proBNP biomarker based on branched AuPd nanocrystals/N-doped honeycombed porous carbon

Tang, Chang; Zhang, Jingxian; Chen, Di-Nan; He, Jia-Wen; Wang, Ai‐Jun; Feng, Jiu‐Ju

doi:10.1016/j.bioelechem.2022.108225

Cited by 24 publications

(8 citation statements)

References 53 publications

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“…The fabricated sensor showed a wide linear range (0.001–100 ng mL –1 ) and LOD of 0.25 pg mL –1 in human serum samples . The same group reported immunosensing of C-reactive protein and N-terminal pro-B-type natriuretic peptide using ultrasensitive electrochemical biosensors …”

Section: Introductionmentioning

confidence: 97%

Yttrium Functionalized Reduced Graphene Oxide Nanocomposite-Based Aptasensor for Ultrasensitive Detection of a Breast Cancer Biomarker

Parihar,

Khan

2023

ACS Appl. Nano Mater.

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Higher cancer mortality rate can be prevented by the early detection of cancer-associated biomarkers and appropriate therapeutic intervention. Recently, electrochemical biosensors have drawn a great deal of interest because of their extremely high sensitivity, selectivity, and affordability in early cancer detection. Herein, we fabricated an aptasensor for the inexpensive and label-free detection of the epidermal growth factor receptor (EGFR) antigen, a biomarker linked to breast cancer. The reduced graphene oxide–yttrium nanocomposite was synthesized and characterized using FTIR, XRD, TEM, SEM, Raman spectroscopy, and UV–vis spectroscopy, which confirmed the synthesis of the nanocomposite. The synthesized material was used for the fabrication of electrochemical aptasensor for the detection of epidermal growth factor receptor antigen. The developed sensor (Y2O3–rGO/Apt/BSA) showed a wide linear detection range (10 fg mL–1 to 100 ng mL–1), a low detection limit of 0.251 fg mL–1, and an excellent sensitivity of 51.96 μA fM–1·cm–2. The aptasensor also provides a straightforward and quick approach to EGFR antigen detection in biological serum samples. The simple and facile synthesis method reported in this work led to the production of a high-purity, water-dispersible yttrium functionalized reduced graphene oxide nanocomposite for cost-effective, faster, and ultrasensitive detection of breast cancer biomarkers using electrochemical aptasensors.

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Section: Introductionmentioning

confidence: 97%

Yttrium Functionalized Reduced Graphene Oxide Nanocomposite-Based Aptasensor for Ultrasensitive Detection of a Breast Cancer Biomarker

Parihar,

Khan

2023

ACS Appl. Nano Mater.

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“…The key constituent of any biosensing system is the recognition element. − Electrochemical biosensors and sensors can be fabricated by electropolymerizing of monomers or coating of the electrode surface with chemically synthesized polymers . Conjugated polymers are a significant class of functional substances that have usually been utilized to construct electrochemical systems due to their attractive and adjustable chemical, electrical, and constructive features. , Polythiophene is one of the most important π-conjugated polymers for biosensors, supercapacitors, electrochromic devices, and thermal conductors because of its good electrical conductivity, mechanical robustness, and environmental stability .…”

Section: Introductionmentioning

confidence: 99%

“…The key constituent of any biosensing system is the recognition element. 18 − 20 Electrochemical biosensors and sensors can be fabricated by electropolymerizing of monomers or coating of the electrode surface with chemically synthesized polymers. 21 Conjugated polymers are a significant class of functional substances that have usually been utilized to construct electrochemical systems due to their attractive and adjustable chemical, electrical, and constructive features.…”

Section: Introductionmentioning

confidence: 99%

Label-Free Electrochemical Immunosensor Based on Conjugated Polymer Film Coated Disposable Electrode for Ultrasensitive Determination of Resistin Potential Obesity Biomarker

Aydın,

Sezgintürk

2024

ACS Appl. Bio Mater.

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A new label-free immunosensor was designed for sensitive detection of resistin obesity biomarker in human biological fluids. To construct a sensing interface, the monomer of double epoxy groups-substituted thiophene (TdiEpx) was synthesized for the fabrication of the biosensing system. A disposable indium tin oxide sheet was first modified by electrochemical polymerization of the TdiEpx monomer, and this robust and novel surface was characterized using different spectroscopic and electrochemical analyses. The double epoxy ends were linked to the amino ends of anti-resistin, and they served as binding points for the covalent binding of biomolecules. The double epoxy ends present in each TdiEpx monomer ensured an extensive surface area, which improved the quantity of attached anti-resistin. The determination of resistin antigen was based on the specific coupling of resistin with anti-resistin, and this interaction hindered the electron transfer reaction. The immunosensor introduced a wide linear range of 0.0125–15 pg/mL, a low detection limit of 4.17 fg/mL, and an excellent sensitivity of 1.38 kohm pg mL–1 cm2. In this study, a sandwich enzyme-linked immunosorbent assay spectrophotometric method was utilized as a reference technique for the quantitative analysis of resistin in human serum and saliva samples. Both measurements in clinical samples displayed correlations and high-correlation coefficients. In addition, this immunosensor had good storage stability, acceptable repeatability and reproducibility, high specificity, and good accuracy. The proposed immunosensor provided a simple and versatile impedimetric immunosensing platform and a promisingly sensitive way for clinical applications.

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“…An important disadvantage of the ELISA technique is that it is inaccessible to the public . Electrochemical bioanalytical approaches have become more relevant and important in biological analysis because of their simplicity, lower cost, quick analysis, and portability, notably their excellent sensitivity. , Label-free electrochemical immunosensors without labels are designed as useful instruments, and they have great promise because of their straightforward detection and easy manufacturing methods. − Lee et al designed an electrochemical competitive immunosensing system consisting of a microfluidic channel and a collector. The analytical principle of the system was based on the competitive binding of anticotinine between cotinine-conjugated horseradish peroxidase and cotinine.…”

Section: Introductionmentioning

confidence: 99%

Carboxyethylsilanetriol-Coated Magnetic Nanoparticles as an Ultrasensitive Immunoplatform for Electrochemical Magnetosensing of Cotinine

Aydın,

Sezgintürk

2024

ACS Biomater. Sci. Eng.

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In the present study, an innovative and simple electrochemical magneto biosensor based on carboxyethylsilanetriol-modified iron oxide (Fe 3 O 4 ) magnetic nanoparticles was designed for ultrasensitive and specific analysis of cotinine, an important marker of smoking. Anticotinine antibodies were covalently immobilized on carboxylic acid-modified magnetic nanoparticles, and the cotinine-specific magnetic nanoparticles created a specific surface on the working electrode surface. The use of magnetic nanoparticles as an immobilization platform for antibodies provided a large surface area for antibody attachment and increased sensitivity. In addition, the advantages of the new immobilization platform were reusing the working electrode numerous times, recording repeatable and reproducible signals, and reducing the necessary volume of biomolecules. The specific interaction between cotinine and cotinine-specific antibodyattached magnetic nanoparticles restricted the electron transfer of the redox probe and changed the impedimetric response of the electrode correlated to the concentration of cotinine. The magneto biosensor had a wide detection range (2−300 pg/mL), a low LOD (606 fg/mL), and an acceptable recovery (97.24−105.31%) in real samples. In addition, the current biosensor's measurement results were in good agreement with those found by the standard liquid chromatography (HPLC) and enzyme-linked immunosorbent assay (ELISA) methods. These results showed that a simple impedimetric immunosensing platform was generated for the cotinine analysis.

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Ultrasensitive label-free electrochemical immunosensor of NT-proBNP biomarker based on branched AuPd nanocrystals/N-doped honeycombed porous carbon

Cited by 24 publications

References 53 publications

Yttrium Functionalized Reduced Graphene Oxide Nanocomposite-Based Aptasensor for Ultrasensitive Detection of a Breast Cancer Biomarker

Yttrium Functionalized Reduced Graphene Oxide Nanocomposite-Based Aptasensor for Ultrasensitive Detection of a Breast Cancer Biomarker

Label-Free Electrochemical Immunosensor Based on Conjugated Polymer Film Coated Disposable Electrode for Ultrasensitive Determination of Resistin Potential Obesity Biomarker

Carboxyethylsilanetriol-Coated Magnetic Nanoparticles as an Ultrasensitive Immunoplatform for Electrochemical Magnetosensing of Cotinine

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