Ultrasensitive, label-free detection of cardiac biomarkers with optical SIS sensor

Diware, Mangesh S.; Cho, Hyun Mo; Chegal, Won; Cho, Yong Jai; Kim, Dong Soo; O, Sang Won; Kim, Kyeong-Suk; Paek, Se‐Hwan

doi:10.1016/j.bios.2016.08.049

Cited by 29 publications

(17 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The excited states of dye molecules make a near-field coupling with bimetallic film for surface plasmons at λem. The non-radiative coupling of the excited molecules into surface plasmons at the dielectric-metal interface, a sort of non-radia decay of a dye molecule, occurs only for those spaced close to the metal film, i.e., th Surface plasmon resonance 0-0.160 µg mL −1 0.068 ng mL −1 [22] Optical solution immersed silicon 0.005-10 ng mL −1 5 and 10 pg mL −1 [45] It is worth noting that the SPCE technologies not only enhance the fluorescent signals detected but also suppress photobleaching due to the near field coupling of excited state dye molecules with thin metal layers that support surface plasmon resonance. To comprehend the superior performance of the SPCE chip over the control chip, let us begin by considering that the LED light source excites the molecules of the dye, the Alexa 488, which are conjugated with the detection antibody (D-Ab@Alexa 488) for the sandwich immune reaction.…”

Section: Resultsmentioning

confidence: 99%

Fluorescence Based on Surface Plasmon Coupled Emission for Ultrahigh Sensitivity Immunoassay of Cardiac Troponin I

Tran

2021

Biomedicines

View full text Add to dashboard Cite

This work demonstrates the quantitative assay of cardiac Troponin I (cTnI), one of the key biomarkers for acute cardiovascular diseases (the leading cause of death worldwide) using the fluorescence-based sandwich immune reaction. Surface plasmon coupled emission (SPCE) produced by non-radiative coupling of dye molecules with surface plasmons being excitable via the reverse Kretschmann format is exploited for fluorescence-based sandwich immunoassay for quantitative detection of cTnI. The SPCE fluorescence chip utilizes the gold (2 nm)-silver (50 nm) bimetallic thin film, with which molecules of the dye Alexa 488 (conjugated with detection antibodies) make a near field coupling with the plasmonic film for SPCE. The experimental results find that the SPCE greatly improves the sensitivity via enhancing the fluorescence signal (up to 50-fold) while suppressing the photo-bleaching, permitting markedly enhanced signal-to-noise ratio. The limit of detection of 21.2 ag mL−1 (atto-gram mL−1) is obtained, the lowest ever reported to date amid those achieved by optical technologies such as luminescence and label-free optical sensing techniques. The features discovered such as ultrahigh sensitivity may prompt the presented technologies to be applied for early diagnosis of cTnI in blood, particularly for emergency medical centers overloaded with patients with acute myocardial infarction who would suffer from time-delayed diagnosis due to insufficient assay device sensitivity.

show abstract

Section: Resultsmentioning

confidence: 99%

Fluorescence Based on Surface Plasmon Coupled Emission for Ultrahigh Sensitivity Immunoassay of Cardiac Troponin I

Tran

2021

Biomedicines

View full text Add to dashboard Cite

show abstract

“…In another study, non-linear optical Stokes ellipsometry was used for imaging the non-linear susceptibility tensor of collagen [227]. Ellipsometric sensors have also been fabricated in conjunction with solution immersed silica immunosensors for the rapid (5 min) determination of cTnI in blood serum [228].…”

Section: Ellipsometric Biosensorsmentioning

confidence: 99%

Recent Progress in Optical Sensors for Biomedical Diagnostics

Pirzada

Altıntaş

2020

Micromachines

View full text Add to dashboard Cite

In recent years, several types of optical sensors have been probed for their aptitude in healthcare biosensing, making their applications in biomedical diagnostics a rapidly evolving subject. Optical sensors show versatility amongst different receptor types and even permit the integration of different detection mechanisms. Such conjugated sensing platforms facilitate the exploitation of their neoteric synergistic characteristics for sensor fabrication. This paper covers nearly 250 research articles since 2016 representing the emerging interest in rapid, reproducible and ultrasensitive assays in clinical analysis. Therefore, we present an elaborate review of biomedical diagnostics with the help of optical sensors working on varied principles such as surface plasmon resonance, localised surface plasmon resonance, evanescent wave fluorescence, bioluminescence and several others. These sensors are capable of investigating toxins, proteins, pathogens, disease biomarkers and whole cells in varied sensing media ranging from water to buffer to more complex environments such as serum, blood or urine. Hence, the recent trends discussed in this review hold enormous potential for the widespread use of optical sensors in early-stage disease prediction and point-of-care testing devices.

show abstract

“…Diware et al developed a solution-immersed silicon biosensor for myocardial infarction early diagnosis. Due to this sensor having a very high sensitivity on the thickness of the biolayer under the nonreflection condition, it would become a candidate for detecting biomarkers or other biological information from humans [ 180 ].…”

Section: Other Biomarker Sensorsmentioning

confidence: 99%

Recent Progress of Biomarker Detection Sensors

Liu

Cui

2020

Research

View full text Add to dashboard Cite

Early cancer diagnosis and treatment are crucial research fields of human health. One method that has proven efficient is biomarker detection which can provide real-time and accurate biological information for early diagnosis. This review presents several biomarker sensors based on electrochemistry, surface plasmon resonance (SPR), nanowires, other nanostructures, and, most recently, metamaterials which have also shown their mechanisms and prospects in application in recent years. Compared with previous reviews, electrochemistry-based biomarker sensors have been classified into three strategies according to their optimizing methods in this review. This makes it more convenient for researchers to find a specific fabrication method to improve the performance of their sensors. Besides that, as microfabrication technologies have improved and novel materials are explored, some novel biomarker sensors—such as nanowire-based and metamaterial-based biomarker sensors—have also been investigated and summarized in this review, which can exhibit ultrahigh resolution, sensitivity, and limit of detection (LoD) in a more complex detection environment. The purpose of this review is to understand the present by reviewing the past. Researchers can break through bottlenecks of existing biomarker sensors by reviewing previous works and finally meet the various complex detection needs for the early diagnosis of human cancer.

show abstract

Ultrasensitive, label-free detection of cardiac biomarkers with optical SIS sensor

Cited by 29 publications

References 51 publications

Fluorescence Based on Surface Plasmon Coupled Emission for Ultrahigh Sensitivity Immunoassay of Cardiac Troponin I

Fluorescence Based on Surface Plasmon Coupled Emission for Ultrahigh Sensitivity Immunoassay of Cardiac Troponin I

Recent Progress in Optical Sensors for Biomedical Diagnostics

Recent Progress of Biomarker Detection Sensors

Contact Info

Product

Resources

About