Ultrasensitive Label- and PCR-Free Genome Detection Based on Cooperative Hybridization of Silicon Nanowires Optical Biosensors

Leonardi, Antonio Alessio; Faro, Maria Josè Lo; Petralia, Salvatore; Fazio, Barbara; Musumeci, Paolo; Conoci, Sabrina; Irrera, Alessia; Priolo, F.

doi:10.1021/acssensors.8b00422

Cited by 67 publications

(63 citation statements)

References 41 publications

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“…Silicon nanowires are emerging in several fields as an innovative building block to overcome different challenges from microelectronics [1][2][3] to energetics [4][5][6], photonics [7][8][9], and sensing [10][11][12]. Starting from microelectronics, a lot of effort has been spent in recent years to find novel solutions that permit to surpass the limitations of the modern-day Moore's law [13].…”

Section: Introductionmentioning

confidence: 99%

CMOS-Compatible and Low-Cost Thin Film MACE Approach for Light-Emitting Si NWs Fabrication

Leonardi

Irrera

2020

Nanomaterials

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Silicon nanowires (Si NWs) are emerging as an innovative building block in several fields, such as microelectronics, energetics, photonics, and sensing. The interest in Si NWs is related to the high surface to volume ratio and the simpler coupling with the industrial flat architecture. In particular, Si NWs emerge as a very promising material to couple the light to silicon. However, with the standard synthesis methods, the realization of quantum-confined Si NWs is very complex and often requires expensive equipment. Metal-Assisted Chemical Etching (MACE) is gaining more and more attention as a novel approach able to guarantee high-quality Si NWs and high density with a cost-effective approach. Our group has recently modified the traditional MACE approach through the use of thin metal films, obtaining a strong control on the optical and structural properties of the Si NWs as a function of the etching process. This method is Complementary Metal-Oxide-Semiconductors (CMOS)-technology compatible, low-cost, and permits us to obtain a high density, and room temperature light-emitting Si NWs due to the quantum confinement effect. A strong control on the Si NWs characteristics may pave the way to a real industrial transfer of this fabrication methodology for both microelectronics and optoelectronics applications.

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Section: Introductionmentioning

confidence: 99%

CMOS-Compatible and Low-Cost Thin Film MACE Approach for Light-Emitting Si NWs Fabrication

Leonardi

Irrera

2020

Nanomaterials

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“…In this scenario, the integration of silicon technologies such as micro‐electromechanical systems and very large‐scale integration, together with microfluidics, is very intriguing to achieve full integration of NA testing protocols on miniaturized and portable diagnostic devices (Fernández‐Carballo et al, 2016; Guarnaccia et al, 2014; Hsieh et al, 2015; Tong et al, 2019). The main advantages of silicon are low heat capacity, good thermal conductivity, good biocompatibility, chemical derivatization, and the possibility to produce patterned structures, which increase the surface‐area ratio, improving the efficiency of NA extraction and detection (Leonardi et al, 2018; Petralia et al, 2015; Valli et al, 2006). 3D printing technology and numerical tools are useful techniques to generate models for the simulation of surface to control the shape and size of pores as well as the distribution of the pore's network inside the sample (Zerhouni, Tarantino, Danas, & Hong, 2018).…”

Section: Introductionmentioning

confidence: 99%

An integrated biosensor platform for extraction and detection of nucleic acids

Sciuto

Petralia

Calabrese

et al. 2020

Biotech & Bioengineering

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The development of portable systems for analysis of nucleic acids (NAs) is crucial for the evolution of biosensing in the context of future healthcare technologies. The integration of NA extraction, purification, and detection modules, properly actuated by microfluidics technologies, is a key point for the development of portable diagnostic systems. In this paper, we describe an integrated biosensor platform based on a silicon–plastic hybrid lab‐on‐disk technology capable of managing NA extraction, purification, and detection processes in an integrated format. The sample preparation process is performed by solid‐phase extraction technology using magnetic beads on a plastic disk, while detection is done through quantitative real‐time polymerase chain reaction (qRT‐PCR) on a miniaturized silicon device. The movement of sample and reagents is actuated by a centrifugal force induced by a disk actuator instrument. The assessment of the NA extraction and detection performance has been carried out by using hepatitis B virus (HBV) DNA genome as a biological target. The quantification of the qRT‐PCR chip in the hybrid disk showed an improvement in sensitivity with respect to the qRT‐PCR commercial platforms, which means an optimization of time and cost. Limit of detection and limit of quantification values of about 8 cps/reaction and 26 cps/reaction, respectively, were found by using analytical samples (synthetic clone), while the results with real samples (serum with spiked HBV genome) indicate that the system performs as well as the standard methods.

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“…In turn, field effect transistor devices can play a key role in the aforementioned applications (e.g., biosensing), as the majority of biomolecules and bioreactions involve charge and potential shifts that can be detected electrically [7][8][9]. Silicon nanowires (SiNWs) do not only provide excellent electrical but also superior optical properties, enabling their usage also as optical biosensors [10][11][12]. Furthermore, modern semiconducting manufacturing techniques offer rewards in terms of miniaturization, parallel sensing, and integration [13,14].…”

Section: Introductionmentioning

confidence: 99%

Surface Modification of Silicon Nanowire Based Field Effect Transistors with Stimuli Responsive Polymer Brushes for Biosensing Applications

et al. 2020

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We demonstrate the functionalization of silicon nanowire based field effect transistors (SiNW FETs) FETs with stimuli-responsive polymer brushes of poly(N-isopropylacrylamide) (PNIPAAM) and poly(acrylic acid) (PAA). Surface functionalization was confirmed by atomic force microscopy, contact angle measurements, and verified electrically using a silicon nanowire based field effect transistor sensor device. For thermo-responsive PNIPAAM, the physicochemical properties (i.e., a reversible phase transition, wettability) were induced by crossing the lower critical solution temperature (LCST) of about 32 °C. Taking advantage of this property, osteosarcomic SaoS-2 cells were cultured on PNIPAAM-modified sensors at temperatures above the LCST, and completely detached by simply cooling. Next, the weak polyelectrolyte PAA, that is sensitive towards alteration of pH and ionic strength, was used to cover the silicon nanowire based device. Here, the increase of pH will cause deprotonation of the present carboxylic (COOH) groups along the chains into negatively charged COO− moieties that repel each other and cause swelling of the polymer. Our experimental results suggest that this functionalization enhances the pH sensitivity of the SiNW FETs. Specific receptor (bio-)molecules can be added to the polymer brushes by simple click chemistry so that functionality of the brush layer can be tuned optionally. We demonstrate at the proof-of concept-level that osteosarcomic Saos-2 cells can adhere to PNIPAAM-modified FETs, and cell signals could be recorded electrically. This study presents an applicable route for the modification of highly sensitive, versatile FETs that can be applied for detection of a variety of biological analytes.

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Ultrasensitive Label- and PCR-Free Genome Detection Based on Cooperative Hybridization of Silicon Nanowires Optical Biosensors

Cited by 67 publications

References 41 publications

CMOS-Compatible and Low-Cost Thin Film MACE Approach for Light-Emitting Si NWs Fabrication

CMOS-Compatible and Low-Cost Thin Film MACE Approach for Light-Emitting Si NWs Fabrication

An integrated biosensor platform for extraction and detection of nucleic acids

Surface Modification of Silicon Nanowire Based Field Effect Transistors with Stimuli Responsive Polymer Brushes for Biosensing Applications

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