Ultrasensitive electrochemical immunosensor based on dual signal amplification process for p16INK4a cervical cancer detection in clinical samples

Duangkaew, Pattasuda; Tapaneeyakorn, Satita; Apiwat, Chayachon; Dharakul, Tararaj; Laiwejpithaya, Somsak; Kanatharana, Proespichaya; Laocharoensuk, Rawiwan

doi:10.1016/j.bios.2015.07.004

Cited by 41 publications

(27 citation statements)

References 29 publications

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“…The enhanced electrochemical response in the oxidation processes indicated the electroconducting properties of the GP/PANI nanocomposite in facilitating the electron transfer between the electrode surface and chloride ion [ 13 ]. After stepwise immobilization the modified electrode with capture antibody (CapAb) (peak c) and Ab-Fe@Au with CFP10 antigen (peak d), the peak currents gradually increase might be due to the signal enhancement of Fe@Au MNPs [ 32 ]. Meanwhile, for the SPGE/GP/PANI/CapAb/BSA/Ab-Fe@Au (peak e), the peak current decrease, showing that the Fe@Au MNPs did not attach on the surface of the electrode.…”

Section: Resultsmentioning

confidence: 99%

Sandwich Electrochemical Immunosensor for Early Detection of Tuberculosis Based on Graphene/Polyaniline-Modified Screen-Printed Gold Electrode

Azmi

Yusof

Kusnin

et al. 2018

Sensors

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A rapid and sensitive sandwich electrochemical immunosensor was developed based on the fabrication of the graphene/polyaniline (GP/PANI) nanocomposite onto screen-printed gold electrode (SPGE) for detection of tuberculosis biomarker 10-kDa culture filtrate protein (CFP10). The prepared GP/PANI nanocomposite was characterized using Fourier transform infrared spectroscopy (FTIR) and field emission scanning electron microscopy (FESEM). The chemical bonding and morphology of GP/PANI-modified SPGE were studied by Raman spectroscopy and FESEM coupled with energy dispersive X-ray spectroscopy, respectively. From both studies, it clearly showed that GP/PANI was successfully coated onto SPGE through drop cast technique. Cyclic voltammetry was used to study the electrochemical properties of the modified electrode. The effective surface area for GP/PANI-modified SPGE was enhanced about five times compared with bare SPGE. Differential pulse voltammetry was used to detect the CFP10 antigen. The GP/PANI-modified SPGE that was fortified with sandwich type immunosensor exhibited a wide linear range (20–100 ng/mL) with a low detection limit of 15 ng/mL. This proposed electrochemical immunosensor is sensitive, low sample volume, rapid and disposable, which is suitable for tuberculosis detection in real samples.

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Section: Resultsmentioning

confidence: 99%

Sandwich Electrochemical Immunosensor for Early Detection of Tuberculosis Based on Graphene/Polyaniline-Modified Screen-Printed Gold Electrode

Azmi

Yusof

Kusnin

et al. 2018

Sensors

View full text Add to dashboard Cite

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“…The positivity detection rate of panels of tumor markers was 95.5% for 95 cases of cancer-positive sera and with a shelf life of at least 35 days [112]. Duangkaew et al also describe the use of functionalized AuNPs in an SPCE, for cancer detection [113]. They used a sandwich approach in which a cervical cancer biomarker (GST-p16) served as a linker, binding antibodies in the carbon electrode and in the AuNPs.…”

Section: General Overview Of Applicationsmentioning

confidence: 99%

“…The sample was then silver-enhanced, increasing the current signal due to the proximity of the nanoparticles. After analysis of 20 clinical samples, the LOD was set at 1.3 ng/mL for GST-p16 protein which is equivalent to 28 cells for HeLa cervical cancer cells [113]. Disposable biosensors employing screen-printing technology have already proven their commercial success for diabetes management due to the multi-billion-dollar glucose monitoring market [114].…”

Section: General Overview Of Applicationsmentioning

confidence: 99%

Gold Nanoparticles for Diagnostics: Advances towards Points of Care

et al. 2016

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The remarkable physicochemical properties of gold nanoparticles (AuNPs) have prompted developments in the exploration of biomolecular interactions with AuNP-containing systems, in particular for biomedical applications in diagnostics. These systems show great promise in improving sensitivity, ease of operation and portability. Despite this endeavor, most platforms have yet to reach maturity and make their way into clinics or points of care (POC). Here, we present an overview of emerging and available molecular diagnostics using AuNPs for biomedical sensing that are currently being translated to the clinical setting.

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“…In a voltammetric setting, two or three electrode electrochemical cell systems together with a potentiostat are used to sense the transduction signal. This method is reportedly used to detect quite a few cancer biomarker with great sensitivity and specificity [15][16][17][18]. In an amperometric technique, the transduction happens through current measurement.…”

Section: Electrochemicalmentioning

confidence: 99%