Ultrasensitive electrochemical detection of miRNA based on polymerization signal amplification

Wang, Qingyu; Sun, Haobo; Wen, Dongxiao; Wang, Lei; Li, Lianzhi; Kong, Jinming; Zhang, Xueji

doi:10.1016/j.talanta.2021.122744

Cited by 24 publications

(4 citation statements)

References 23 publications

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“…When t H is beyond 35 min, the value of I p becomes constant, demonstrating the completion of the hybridization reaction. It is noticeable that the homogeneous hybridization time is shorter than those of the biosensors based on the solid/liquid heterogeneous hybridization reaction − because the hybridization in the homogeneous solution has faster base-paring reaction kinetics.…”

Section: Resultsmentioning

confidence: 99%

Specific Adsorption of Magnetic Fe₃O₄@hydroxyapatite Nanocomposites to a Hybridized Duplex for Immobilization and Label-Free Electrochemical Biosensing of MicroRNA

Chen

Yang

et al. 2023

ACS Appl. Nano Mater.

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The unique discriminating ability of hydroxyapatite (HAP) to single- and double-stranded nucleic acid implies its potential as a biosensing material for nucleic acid analysis. Herein, a novel immobilization- and label-free electrochemical strategy has been developed for miRNA let-7a detection based on the specific adsorption of magnetic Fe3O4@hydroxyapatite (Fe3O4@HAP) to a duplex structure. Typically, the highly efficient homogeneous reaction between probe DNA (pDNA) and target miRNA let-7a produces the nucleic acid duplex, which is specifically adsorbed on the surface of Fe3O4@HAP. Thereafter, the electroactive intercalator Nile blue (NB) is introduced as the label-free signal molecule and inserts between the stacked base pairs of the pDNA–miRNA duplex. Thus, the Fe3O4@HAP bearing pDNA–miRNA and numerous intercalated NBs are adsorbed by the magnetic glassy carbon electrode, giving rise to an intense sensing signal without the need of nuclease-assisted cyclic amplification. With this advantage, the sensing system shows a wide linear range from 1.0 fM to 100 nM and a low detection limit of 0.051 fM for miRNA let-7a analysis. What is more, the synergy of the base-pairing-driven hybridization, the specific intercalation of NB to the duplex structure, and the selective adsorption of Fe3O4@HAP contribute to a high sensing specificity of the biosensing strategy. The sensing method provides a state-of-the-art strategy for the sensitive and facile bioanalysis and clinical diagnosis of miRNA.

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Section: Resultsmentioning

confidence: 99%

Specific Adsorption of Magnetic Fe₃O₄@hydroxyapatite Nanocomposites to a Hybridized Duplex for Immobilization and Label-Free Electrochemical Biosensing of MicroRNA

Chen

Yang

et al. 2023

ACS Appl. Nano Mater.

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“…Many solid phase hybridization assays based on efficient signal amplication (e.g., nanomaterial-based amplication [24][25][26][27][28][29][30][31] and electrochemical methods [32][33][34][35][36][37] ) have improved the sensitivity of miRNA analysis. However, these assays usually require complex fabrication, cumbersome procedures and/or expensive capture-modied probes.…”

Section: Introductionmentioning

confidence: 99%

Precise quantification of microRNAs based on proximity ligation of AuNPs-immobilized DNA probes

Li,

Xiao,

Yuan

et al. 2024

Anal. Methods

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“…The fascinating and constantly expanding area of nanotechnology allows the creation of extremely microscopic substances (Azzazy et al, 2012;Gul et al, 2024;Kaur, 2024). Applications of nanotechnology have improved impact on electronics (Pyun et al, 2023), therapeutics (Al-Taie and Özcan Bülbül, 2024; Gul et al, 2024), food (Nanda et al, 2024;Wang et al, 2024), sustainable agriculture (Mohammadi et al, 2024), chemical and pharmaceutical industries (Chakraborty et al, 2023;Iqubal et al, 2022), and environmental health (Pamanji et al, 2024;Wang et al, 2024). Innovative nanomaterial synthesis methods have advanced nanoscience and technology throughout the past decade.…”

Section: Introductionmentioning

confidence: 99%

Magnesium Sulfide Nanoparticles of Hordeum vulgare: Green Synthesis and their nano- nutrient impact on seed priming effect, germination, root and shoot length of Brassica nigra and Trigonella foenum-graecum

Elizabeth,

Devi,

Makam

et al. 2024

Preprint

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This work describes the green synthetic process for magnesium sulfide nanoparticles (MgS NPs) using an extract from Hordeum vulgare leaves. An examination of the MgS NPs was performed utilizing X-ray diffraction (XRD), UV-visible spectroscopy, and scanning electron microscopy (SEM) techniques. The MgS NPs generated were spherical and had a high level of purity. They had a band gap of 2.0 eV, a uniform distribution, and an average crystal size of 14 nm. The MgS nanoparticles synthesized exhibited several geometrical morphologies, including spherical, rod-shaped, and bean-shaped structures. The nanoparticles exhibited an average size of 5 nm, with a band gap of precisely 4.85 eV. The efficacy of MgS NPs on Brassica nigra and Trigonella foenum-graecum for seed priming, germination rate and time, root length, and shoot length has been assessed using different doses. Optimal germination occurs at concentrations of 15mg/100ml and 20mg/100ml, while germination is impeded when the concentration surpasses 30mg/100ml. MgS NPs exhibit a diminutive size and elevated reactivity, which allows them to augment the water absorption and nutrient control capacities of seeds. Consequently, this promotes the germination process and plant growth by decreasing the average duration of germination. Seeds of Brassica nigra and Trigonella foenum-graecum that had been subjected to treatment with MgS NPs had enhanced average root and shoot lengths, as well as accelerated germination. The results of this study suggest various promising opportunities for investigating the application of environmentally friendly nanotechnology to improve agricultural practices.

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Ultrasensitive electrochemical detection of miRNA based on polymerization signal amplification

Cited by 24 publications

References 23 publications

Specific Adsorption of Magnetic Fe₃O₄@hydroxyapatite Nanocomposites to a Hybridized Duplex for Immobilization and Label-Free Electrochemical Biosensing of MicroRNA

Specific Adsorption of Magnetic Fe₃O₄@hydroxyapatite Nanocomposites to a Hybridized Duplex for Immobilization and Label-Free Electrochemical Biosensing of MicroRNA

Precise quantification of microRNAs based on proximity ligation of AuNPs-immobilized DNA probes

Magnesium Sulfide Nanoparticles of Hordeum vulgare: Green Synthesis and their nano- nutrient impact on seed priming effect, germination, root and shoot length of Brassica nigra and Trigonella foenum-graecum

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Ultrasensitive electrochemical detection of miRNA based on polymerization signal amplification

Cited by 24 publications

References 23 publications

Specific Adsorption of Magnetic Fe3O4@hydroxyapatite Nanocomposites to a Hybridized Duplex for Immobilization and Label-Free Electrochemical Biosensing of MicroRNA

Specific Adsorption of Magnetic Fe3O4@hydroxyapatite Nanocomposites to a Hybridized Duplex for Immobilization and Label-Free Electrochemical Biosensing of MicroRNA

Precise quantification of microRNAs based on proximity ligation of AuNPs-immobilized DNA probes

Magnesium Sulfide Nanoparticles of Hordeum vulgare: Green Synthesis and their nano- nutrient impact on seed priming effect, germination, root and shoot length of Brassica nigra and Trigonella foenum-graecum

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Specific Adsorption of Magnetic Fe₃O₄@hydroxyapatite Nanocomposites to a Hybridized Duplex for Immobilization and Label-Free Electrochemical Biosensing of MicroRNA

Specific Adsorption of Magnetic Fe₃O₄@hydroxyapatite Nanocomposites to a Hybridized Duplex for Immobilization and Label-Free Electrochemical Biosensing of MicroRNA