Ultrasensitive electrochemical biosensor for protein detection based on target-triggering cascade enzyme-free signal amplification strategy

Electrochemical Signal Amplification Strategies and Their Use in Olfactory and Taste Evaluation

“…Ling et al [ 143 ] constructed an electrochemical biosensor for efficient protein detection based on the molecular recognition between an aptamer and a target utilizing a target departure signal amplification strategy. They hybridized the auxiliary DNA1 with an aptamer, forming a double-strand, and then used the high affinity between the aptamer and target to release the auxiliary DNA1 and trigger the DNA1 circuit, eventually forming a large number of hairpin-shaped DNA3 on the electrode surface.…”

Allosteric aptasensor-initiated target cycling and transcription amplification of light-up RNA aptamer for sensitive detection of protein

“…Aptamers are short single-stranded DNA or RNA that can interact with corresponding targets with high affinity and specificity [17] , [18] . Based on the structure of nucleic acid, aptamers can be used for allosteric regulation to develop aptamer-based amplification assays [19] , such as recombinase polymerase amplification (RPA) [20] , rolling circle amplification (RCA) [21] , [22] , hybridization chain reaction (HCR) [23] , [24] , CRISPR-Cas13a/12a amplification reaction [25] , [26] . However, the sensitivity for most methods is at the picomolar level [27] .…”

A flexible mesoporous Cu doped FeSn–G–SiO2composite based biosensor for microalbumin detection

“…Because of their excellent sensitivity, very low detection limit, and outstanding selectivity, electrochemical sensors are used for microalbumin detection. [4][5][6][7] Iron-based alloys and compounds have provided an excellent foundation for biosensing applications; iron-tin alloy-based sensors are capable of electrically detecting a eld, and are becoming increasingly important for sensing applications. 8 Improved biosensing elements are one eld of biosensor development.…”