Ultrasensitive electrical detection of protein using nanogap electrodes and nanoparticle-based DNA amplification

DNA for Non-nucleic Acid Sensing

“…This approach was used successfully for detection of biomarkers (Stoeva et al 2006;Goluch et al 2006;Nam et al 2002) or viral protein (Yin et al 2011). There exists the possibility of labelling the complementary strand barcode used to measure the amount of nanoparticles and thus targets by conventional methods, usually optical (fluorescent labelling with fluorescein: Cao et al 2010;Zhang et al 2009;chemiluminescence: Peng et al 2011) but using more of the conductance modulation nanogap (Chang et al 2007). This amplification approach using nano-objects has achieved sensing devices with detection thresholds 3-4 orders of magnitude below what can be achieved with an ELISA.…”

RNA and DNA Diagnostics

“…This approach was used successfully for detection of biomarkers (Stoeva et al 2006;Goluch et al 2006;Nam et al 2002) or viral protein (Yin et al 2011). There exists the possibility of labelling the complementary strand barcode used to measure the amount of nanoparticles and thus targets by conventional methods, usually optical (fluorescent labelling with fluorescein: Cao et al 2010;Zhang et al 2009;chemiluminescence: Peng et al 2011) but using more of the conductance modulation nanogap (Chang et al 2007). This amplification approach using nano-objects has achieved sensing devices with detection thresholds 3-4 orders of magnitude below what can be achieved with an ELISA.…”

Aptamer‐Based Bioanalytical Assays: Amplification Strategies

“…In particular, metallic or magnetic nanoparticles have been coupled to different biomolecules, such as DNA or antibodies (Yao et al, 2006;Centi et al, 2007a;Chang et al, 2007). Nanoparticles can be used as electrochemical markers, as catalytic labels for the enlargement of nanoparticles, or as a capturing agent in the case of magnetic nanoparticles coated with biomolecules.…”