Ultrasensitive Diagnosis for an Anthrax‐Protective Antigen Based on a Polyvalent Directed Peptide Polymer Coupled to Zinc Oxide Nanorods

Park, Hye-Yeon; Gedi, Vinayakumar; Kim, Joungmok; Park, Hae-Chul; Han, Sung‐Hwan; Yoon, Moon‐Young

doi:10.1002/adma.201103284

Cited by 20 publications

(14 citation statements)

References 25 publications

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“…For these reasons, ZnO nanomaterials function to enhance fluorescent signals in the detection of fluorescent-labeled biomolecules , for use in biosensors and biomedical applications. In previous research, ZnO nanomaterials were confirmed to increase fluorescence signals . Herein, a nanoporous ZnO platform was obtained by cation exchange (Zn 2+ ) with CdO architectures, and its XRD pattern is shown in Figure A.…”

Section: Resultsmentioning

confidence: 95%

Ultrasensitive Fluorescence Detection of Alzheimer’s Disease Based on Polyvalent Directed Peptide Polymer Coupled to a Nanoporous ZnO Nanoplatform

et al. 2019

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Amyloid-beta 42 (Aβ42), the key biomarker of Alzheimer’s disease (AD), aggregates to form neurotoxic amyloid plaques. In this work, we modified two fluorescein isothiocyanate-labeled Aβ42-targeting peptides and designed an Aβ42-specific ultrasensitive polyvalent-directed peptide polymer (PDPP) to enhance AD diagnosis sensitivity. The dissociation constant of Aβ42 by PDPP was 103-fold higher than the single-site-directed peptide. The improved binding was due to the ability of PDPP to detect multiple receptors on the target. The power of the PDPP diagnostic probe was verified in its application to detect Aβ42 in cerebrospinal fluid (CSF), which showed a lower limit of detection (LOD) in the fg mL –1 range that is more sensitive than detection by antibodies or single peptides. In addition, we present a novel ultrasensitive diagnostic system using an array of nanoporous ZnO nanoparticles, which play a role in fluorescence signal amplification, to further improve AD diagnosis sensitivity. We enhanced the signal on the basis of the properties of nanoporous ZnO nanoparticles and measured and quantified an ultralow concentration (ag mL –1 range) of Aβ42. This PDPP coupled to the nanoporous ZnO-based system is a novel approach to AD diagnosis that might also be useful for the detection of other target biomarkers and clinical applications.

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Section: Resultsmentioning

confidence: 95%

Ultrasensitive Fluorescence Detection of Alzheimer’s Disease Based on Polyvalent Directed Peptide Polymer Coupled to a Nanoporous ZnO Nanoplatform

et al. 2019

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“…The introduction of nanotechnology has provided enormous opportunities to enhance the performance of biosensors. 27 A polyvalent peptide polymer that was coupled with zinc oxide nanorods allowed for the development of an ultrasensitive fluorescence sensor with a LOD of 15 zM for PA. 28 An electrical graphene aptasensor enabled PA detection with a LOD of 1.2 aM. 29 The combined use of an electrochemical immunosensor with bismuth nanocomposites and cadmiumion-functionalized titanium phosphates enabled detection even at a volume of as low as 50 pg/mL in 35 min.…”

Section: Anthracismentioning

confidence: 99%

“…The introduction of nanotechnology has provided enormous opportunities to enhance the performance of biosensors . A polyvalent peptide polymer that was coupled with zinc oxide nanorods allowed for the development of an ultrasensitive fluorescence sensor with a LOD of 15 zM for PA . An electrical graphene aptasensor enabled PA detection with a LOD of 1.2 aM .…”

Section: Biosensors For Anthrax Toxinsmentioning

confidence: 99%

Biosensors for the Detection of Bacillus anthracis

Wang

Cui

et al. 2021

Acc. Chem. Res.

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Conspectus Bacillus anthracis, present in two forms of vegetative cells and spores, is a pathogen that infects humans through contact with infected animals or contaminated animal products and is also maliciously used in terrorist acts. Therefore, a rapid and sensitive test for B. anthracis is necessary but challenging. The challenge comes from the following aspects: an accurate distinction of B. anthracis from other Bacillus species due to their high genomic similarity and the horizontal gene transfer between Bacillus members; direct detection of the B. anthracis spores without damaging them for component extraction to avoid the risk of spore atomization; and the rapid detections of B. anthracis in complex samples, such as soil and suspicious powders, without sample pretreatments and expensive large-scale equipment. Although culturing B. anthracis from samples is the conventional method for the detection of B. anthracis, it is time-consuming and the detection results would not be easy to interpret because many Bacillus species share similar phenotypic features such as a lack of motility and hemolysis, resistance to gamma phages, and so on. Intensive and extensive effort has been expended to develop reliable detection technologies, among which biosensors exhibit comprehensive advantages in terms of sensitivity, specificity, and portability. Here, we briefly review the research progress, providing highlights of the latest achievements and our own practice and experience. The contents can be summarized in three aspects: the discovery of detection targets, including genes, toxins, and other components; the creation of molecular recognition elements, such as monoclonal antibodies, single-chain antibody fragments, specific peptides, and aptamers; and the design and construction of biosensing systems by the integration of appropriate molecular recognition elements and transducer devices. These sensor devices have their own characteristics and different principles. For example, the surface plasmon resonance biosensor and quartz crystal microbalance biosensor are very sensitive, while the multiplex PCR-on-a-chip can detect multitargets. Biosensors for direct spore detection are highly recommended because they are not only fast but also avoid contamination from aerosol-containing spores. The introduction of nanotechnology has significantly improved the performance of biosensors. Superparamagnetic nanoparticles and phage-displayed gold nanoparticle ligand peptides have made the results of spore detection visible to the naked eye. Because of space constraints, many advanced biosensors for B. anthracis are not described in detail but are cited as references. Although biosensors provide a variety of options for various application scenarios, the challenges have not been fully addressed, which leaves room for the development of more advanced and practical B. anthracis detection means.

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“…60 Several assays that measure 1 or more of the 3 toxin proteins have been published-for example, antibody microarrays, 61 microwave-accelerated metal-enhanced fluorescence, 62 ELISA, 8 europium nanoparticles based immunoassay, 63 or detection based on polyvalent directed peptide polymer. 64 An activity-based immunoassay for LF has been described by Bagramyan and Kalkum. 65 The method uses commercially available fluorophore-tagged peptides as substrates for LF activity measurement.…”

Section: Clostridial Neurotoxinsmentioning

confidence: 99%

Mass Spectrometric Detection of Protein-Based Toxins

Åberg

Björnstad

Hedeland

2013

Biosecurity and Bioterrorism: Biodefense Strategy, Practice, an

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This review focuses on mass spectrometric detection of protein-based toxins, which are among the most toxic substances known. Special emphasis is given to the bacterial toxins botulinum neurotoxin from Clostridium botulinum and anthrax toxins from Bacillus anthracis as well as the plant toxin ricin produced by Ricinus communis. A common feature, apart from their extreme toxicity, is that they are composed of 2 polypeptide chains, one of which is responsible for cell uptake and another that has enzymatic function with the ability to destroy basic cellular functions. These toxins pose a threat, both regarding natural spread and from a terrorism perspective. In order for public health and emergency response officials to take appropriate action in case of an outbreak, whether natural or intentional, there is a need for fast and reliable detection methods. Traditionally, large molecules like proteins have been detected using immunological techniques. Although sensitive, these methods suffer from some drawbacks, such as the risk of false-positives due to cross-reactions and detection of inactive toxin. This article describes recently developed instrumental methods based on mass spectrometry for the reliable detection of botulinum neurotoxins, anthrax toxins, and ricin. Unequivocal identification of a protein toxin can be carried out by mass spectrometry-based amino acid sequencing. Furthermore, in combination with antibody affinity preconcentration and biochemical tests with mass spectrometric detection demonstrating the toxin's enzymatic activity, very powerful analytical methods have been described. In conclusion, the advent of sensitive, easily operated mass spectrometers provides new possibilities for the detection of protein-based toxins.

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Ultrasensitive Diagnosis for an Anthrax‐Protective Antigen Based on a Polyvalent Directed Peptide Polymer Coupled to Zinc Oxide Nanorods

Cited by 20 publications

References 25 publications

Ultrasensitive Fluorescence Detection of Alzheimer’s Disease Based on Polyvalent Directed Peptide Polymer Coupled to a Nanoporous ZnO Nanoplatform

Ultrasensitive Fluorescence Detection of Alzheimer’s Disease Based on Polyvalent Directed Peptide Polymer Coupled to a Nanoporous ZnO Nanoplatform

Biosensors for the Detection of Bacillus anthracis

Mass Spectrometric Detection of Protein-Based Toxins

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