Ultrasensitive detection of Vibrio cholerae O1 using microcantilever-based biosensor with dynamic force microscopy

Sungkanak, U; Sappat, Assawapong; Wisitsoraat, Anurat; Promptmas, Chamras; Tuantranont, Adisorn

doi:10.1016/j.bios.2010.06.024

Cited by 59 publications

(33 citation statements)

References 34 publications

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“…The resonant frequency of the cantilever changes due to induced mechanical bending upon an increase in mass on the sensor surface. Microcantilever sensors have been developed for the detection of various whole bacteria, including Escherichia coli O157:H7 (63,64), Salmonella Typhimurium (65), Vibrio cholerae (66), and the biowarfare agent Francisella tularensis (67). The recently developed piezoelectric-excited millimeter-size cantilevers (PEMC) using antibodies as bioreceptors have been able to detect as few as one E. coli cell in buffer (68) and one hundred Listeria monocytogenes cells in milk (69).…”

Section: Mechanical Biosensorsmentioning

confidence: 99%

Biosensors for Whole-Cell Bacterial Detection

et al. 2014

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SUMMARY Bacterial pathogens are important targets for detection and identification in medicine, food safety, public health, and security. Bacterial infection is a common cause of morbidity and mortality worldwide. In spite of the availability of antibiotics, these infections are often misdiagnosed or there is an unacceptable delay in diagnosis. Current methods of bacterial detection rely upon laboratory-based techniques such as cell culture, microscopic analysis, and biochemical assays. These procedures are time-consuming and costly and require specialist equipment and trained users. Portable stand-alone biosensors can facilitate rapid detection and diagnosis at the point of care. Biosensors will be particularly useful where a clear diagnosis informs treatment, in critical illness (e.g., meningitis) or to prevent further disease spread (e.g., in case of food-borne pathogens or sexually transmitted diseases). Detection of bacteria is also becoming increasingly important in antibioterrorism measures (e.g., anthrax detection). In this review, we discuss recent progress in the use of biosensors for the detection of whole bacterial cells for sensitive and earlier identification of bacteria without the need for sample processing. There is a particular focus on electrochemical biosensors, especially impedance-based systems, as these present key advantages in terms of ease of miniaturization, lack of reagents, sensitivity, and low cost.

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Section: Mechanical Biosensorsmentioning

confidence: 99%

Biosensors for Whole-Cell Bacterial Detection

et al. 2014

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“…Gfeller et al (2005) were able to detect E. coli, which is an indicator of fecal pollution of water and food products, with the help of a cantilever coated with agarose; the use of nanomechanical sensing device was able to detect active growth of E. coli cells within 1 h which is significantly faster than any conventional plating method which requires at least 24 h (Sozer and Kokini 2009). Sungkanak et al (2010) demonstrated a cantilever-based cholera sensor; atomic force microscope (AFM) was used to measure the cantilever's resonance frequency shift due to mass of cell bound on microcantilever surface; the microcantilever-based sensor has a detection limit of 1 × 10 3 CFU/ml and a mass sensitivity of 146.5 pg/Hz, which is at least two orders of magnitude lower than other reported techniques.…”

Section: Conductometric 79 Cfu/mlmentioning

confidence: 98%

Nanobiosensors in Food Science and Technology

Mendoza-Madrigal

Chanona-Pérez

Guadarrama-Fernández

et al. 2015

Food Nanoscience and Nanotechnology

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“…A disposable immunosensor has been found to detect eight colonies of V. cholerae bacterium in hand-pump and seawater, and 80 CFU/mL in sewer water and tap water [9]. A cantilever based cholera sensor has been developed by immobilization of antibody of V. cholerae O1 onto gold-coated microcantilever surface and the resonance frequency shift due to bacteria binding has been measured by dynamic force microscopy as a function of V. cholerae concentration ranging from 1 10 3 to 1 10 7 CFU/mL [10]. Anti-CT antibody has been adsorbed onto gold nanoparticles dispersed onto a polytyramine-modi- Full Paper fied gold electrode that can detect CT upto 9 10 À20 M by potential-step capacitance [11].…”

Section: Introductionmentioning

confidence: 99%

Sol‐Gel Derived Nanostructured Metal Oxide Platform for Bacterial Detection

et al. 2011

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O1 gene based 24-mer single stranded deoxyribonucleic acid probe (ssDNA) has been immobilized onto sol-gel derived nanostructured zirconium oxide (NanoZrO 2 ) film fabricated onto indium-tin-oxide (ITO) coated glass plate to detect Vibrio cholerae. The X-ray diffraction and Atomic Force Microscopy techniques have been used to characterize the nanostructured ZrO 2 (particle size of~30-40 nm) and the ssDNA/ZrO 2 bioelectrode. The hybridization of ssDNA/ZrO 2 bioelectrode with the complementary and genomic DNA has been investigated using differential pulse voltammetry. The results of electrochemical studies suggest that electro-active and cationic NanoZrO 2 provides an effective surface to bind with the phosphate group of DNA resulting in enhanced electron transport. The ssDNA/NanoZrO 2 bioelectrode shows a detection range from 1 10 À8 to 10 nM of complementary DNA of V. cholerae within 60 s of hybridization time at 25 8C using methylene blue as an electroactive indicator. This O1 gene based metal oxide (ZrO 2 ) sensor exhibits sensitivity for ssDNA/NanoZrO 2 /ITO bioelectrode as 0.48 mA/nM cm À2 for complementary DNA and 2.34 mA/nM cm À2 for genomic DNA with regression coefficients (R) of 0.991 and 0.995, respectively. This DNA bioelectrode is stable for about 15 weeks when stored at 4 8C.

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Ultrasensitive detection of Vibrio cholerae O1 using microcantilever-based biosensor with dynamic force microscopy

Cited by 59 publications

References 34 publications

Biosensors for Whole-Cell Bacterial Detection

Biosensors for Whole-Cell Bacterial Detection

Nanobiosensors in Food Science and Technology

Sol‐Gel Derived Nanostructured Metal Oxide Platform for Bacterial Detection

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