Ultrasensitive detection of proteins and antibodies by absorption-based laser wave-mixing detection using a chromophore label

Lopez, Mirna M.; Atherton, Adrian A.; Tong, William G.

doi:10.1016/j.ab.2009.12.019

Cited by 7 publications

(3 citation statements)

References 22 publications

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“…In addition to neurotransmitters, wave mixing also offers excellent detection limits in the zepto-mole range for antigens and proteins. 3 Laser wave mixing is also effective in monitoring small changes at trace concentration levels of environmental contaminants that are regulated because of their hazardous nature. For example, malachite green is a parasiticide used illegally in aquaculture that has been banned due to toxic and carcinogenic effects, as well as evidence of developmental abnormalities in mammals with increased exposure over time.…”

Section: Resultsmentioning

confidence: 99%

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Zepto-mole detection in microfluidics by novel nonlinear multi-photon laser wave-mixing spectroscopy for biomedical and environmental applications

et al. 2014

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Laser wave-mixing detectors for microfluidics are presented as compact systems for parts-per-quadrillion-level detection of biomedical and environmental samples. Wave mixing offers unique advantages including absorption-based detection of micrometer-thin samples, generation of coherent laser-like signal beams, and sensitive detection of both fluorescing and non-fluorescing samples. Biomarkers could be detected in their native forms without labels, or if labels are desired, one could use both fluorophores and chromophores as labels. By coupling low-power (mW) solid-state lasers and microfluidics and microarrays, one can design compact optical systems that can be battery powered and used in the field for real-time fingerprinting of chemicals.

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Section: Resultsmentioning

confidence: 99%

“…[1][2][3][4][5][6][7] Trace elemental analysis has been accomplished by using sub-Doppler four wave-mixing spectroscopy with a variety of gas-phase atomizers. Coupled with a DC cathode plasma atomizer, hyperfine splitting can be resolved to yield isotope ratio information.…”

Section: Introductionmentioning

confidence: 99%

Zepto-mole detection in microfluidics by novel nonlinear multi-photon laser wave-mixing spectroscopy for biomedical and environmental applications

et al. 2014

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“…In the direct scheme, the optical properties of an analyte, such as refractive index (RI), absorption or emission, are measured directly. In the indirect scheme, the color or fluorescence of an immobilized label compound, or any other optically-detectable bioprocess, are monitored [35,36]. In recent years, interrogation techniques have further advanced with the use of evanescent-wave spectroscopy and surface-plasmon resonance sensors [3,37].…”

Section: Evanescent-wave Chemical Sensing With Fiber Ring Cavitiesmentioning

confidence: 99%

“Fiber-Optic Cavities for Physical and Chemical Sensing”

Avino¹

2013

TOOPTSJ

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Fiber-optic resonators can be used as ideal mechanical probes and chemical sensors owing to their unique sensitivity to static and dynamic strain applied to the fiber as well as to optical absorption or refractive index changes in the surrounding medium when the evanescent or the internal light field is exposed. The intrinsic properties of high-finesse fiber cavities, either based on Bragg-grating structures or closed fiber rings, are discussed for quasi-static and dynamic strain sensing. Laser illumination and high precision laser frequency-locking techniques are considered for low-noise, fast and wide dynamic range active interrogation. Also, possible detection schemes for liquid sensing and spectroscopy using fiber resonators are illustrated using single-frequency tunable lasers or supercontinuum comb generators as light sources.

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