Ultrasensitive detection of plant hormone abscisic acid-based surface-enhanced Raman spectroscopy aptamer sensor

Zhang, Yanyan; Li, Linze; Zhang, Hao; Shang, Junjian; Li, Can; Naqvi, Syed Muhammad Zaigham Abbas; Birech, Zephania; Hu, Jiandong

doi:10.1007/s00216-022-03923-w

Cited by 10 publications

(1 citation statement)

References 39 publications

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“…For example, Palanco et al (2016) reported that detection of chemical components in onion epidermal cells after incubation with AgNO 3 solution can transfer Ag nanoparticles (AgNPs) to plants as a basic component of plasmon nanosensors by ingesting metal salt solution. Zhang, Li, et al (2022) used aptamer sensors based on SERS and applied them to the highly sensitive detection of the plant hormone abscisic acid. Fang et al (2022) demonstrated that SERS combined with chemical imaging analysis enabled early diagnosis of apple tree rot disease, showing that under apple Valsa canker stress, cellulose and lignin at the phloem disease–healthy junction significantly reduced.…”

Section: Introductionmentioning

confidence: 99%

Nondestructive determination of carotenoids in kiwifruit leaves infected with Pseudomonas syringae pv. actinidiae by surface‐enhanced Raman spectroscopy combined with chemical imaging

et al. 2023

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Surface-enhanced Raman spectroscopy (SERS), a noninvasive and nondestructive technique, was used to distinguish healthy and cankered kiwifruit leaves. Silver nanoparticles with iodide and calcium ions (Ag@ICNPs) were synthesized as SERS substrates and significantly enhanced the characteristic signal peaks of carotenoids.Due to its simple operation and high sensitivity, the detection process is straightforward and efficient. Using a 532 nm laser excitation source, spectra were collected from the leaf surface over a wavelength range of 400-2000 cm −1 , and the characteristic carotenoid peak at 1520 cm −1 was selected for integral imaging. Detection of changes in carotenoid spectral characteristics on the surface of kiwifruit leaves could be used as an early warning of kiwifruit canker. Raman spectroscopy and high-performance liquid chromatography confirmed the existence of two main carotenoids, lutein and β-carotene, and their intensity changed significantly following infection, especially in the later stages of infection. The results of principal component analysis and support vector machine (SVM) analysis of preprocessed spectra showed that samples of healthy, early disease and advanced disease leaves clustered separately, and the accuracy of SVM classification was up to 98%. The results of chemical imaging showed that the average carotenoid signal decreased with extended incubation time and there were significant differences among healthy, early disease and advanced disease stages. Therefore, SERS can be used as a general tool for in situ nondestructive determination of surface carotenoid changes in kiwifruit leaves during disease, and thereby effectively monitor the nutritional quality of kiwifruit before harvest.

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Section: Introductionmentioning

confidence: 99%