Ultrasensitive detection of microRNA based on a homogeneous label-free electrochemical platform using G-triplex/methylene blue as a signal generator

Zhao, Lingli; Pan, Huiyu; Zhang, Xin‐Xiang; Zhou, Ying‐Lin

doi:10.1016/j.aca.2020.04.037

Cited by 28 publications

(11 citation statements)

References 40 publications

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“…As shown in Figure 2A , 10 μM MB had a high reduction current (curve a). When G3 was mixed with MB at 1:1, the current signal was reduced by 56.5% (curve b), which was consistent with the published result ( Zhao et al, 2020a ; Chen et al, 2021 ). The current drop was because G3 was bound to MB to form a G3/MB complex, which could significantly decrease the diffusion rate and inhibit the reduction of MB at electrodes.…”

Section: Resultssupporting

confidence: 91%

“…Previous experiments have shown that a higher concentration of G3 resulted in a lower current of MB, and the best ratio of G3 and MB was 1:1 (Zhao et al, 2020a;Chen et al, 2021). Therefore, in this experiment, we used the same concentration of 10 μM for MB, G3, and C-DNA.…”

Section: Feasibility Of the Analytical Strategymentioning

confidence: 99%

“…Furthermore, molecular dynamics simulations indicated that intercalation was the major mode of interaction between G3 and MB (Zhao et al, 2019). Since the first report, the G3/MB composite has been proved to be an universal, simple, sensitive and efficient homogeneous signal readout element for electrochemical biosensors (Zhao et al, 2020a;Chen et al, 2021).…”

Section: Introductionmentioning

confidence: 98%

See 2 more Smart Citations

A Homogeneous Label-Free Electrochemical microRNA Biosensor Coupling With G-Triplex/Methylene Blue Complex and λ-Exonuclease-Assisted Recycling Amplification

et al. 2021

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A novel homogeneous label-free electrochemical biosensor using G-triplex/methylene blue (G3/MB) complex as the signal generator together with an amplification assisted by the λ-exonuclease (λ-Exo) has been successfully constructed for ultrasensitive microRNA (miRNA) detection. An integrated microelectrode was designed to realize the miniaturization of the homogeneous electrochemical assay. Taking advantage of G3, that can specifically bind with MB and decrease its diffusion current, a single-stranded functional DNA hairpin structure was designed as the bio-recognition probe. The probe consisted of G3, eight bases to block G3, and the complementary sequences of the target miRNA. Here we chose miRNA141—a potentially diagnostic biomarker of prostate cancer as the model target. The presence of miRNA141 could hybridize with the probe DNA to form a double-stranded structure with a 5′-phosphorylated terminus. Then λ-Exo was adopted to digest mononucleotides from the 5′-end, leading to the release of G3 part and miRNA141. The released miRNA could hybridize with another probe to trigger the cycling process, while the released G3 could therefore interact with MB to cause a detectable decrease of diffusion current. The proposed strategy showed a low detection limit of 16 fM and an excellent specificity to discriminate single-base mismatches. Furthermore, this sensor was applied to detect miRNA141 from diluted human serum samples, indicating that it has great potential in the application of nucleic acid detection in real samples.

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Section: Resultssupporting

confidence: 91%

Section: Feasibility Of the Analytical Strategymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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A Homogeneous Label-Free Electrochemical microRNA Biosensor Coupling With G-Triplex/Methylene Blue Complex and λ-Exonuclease-Assisted Recycling Amplification

et al. 2021

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“…The peak current of MB redox process was proportional to miR-21 concentration, achieving low detection limit of 0.02 fM. On the other hand, a newly discovered G-triplex that can specifically bind with MB was applied in electrochemical detection of let-7a by coupling with two-stage isothermal exponential amplification reaction, displaying ultralow detection limit of 0.45 fM and high specificity [50].…”

Section: Intercalation Of Redox Mediatormentioning

confidence: 99%

Recent Progress in Nanomaterials Modified Electrochemical Biosensors for the Detection of MicroRNA

Low

Chai

et al. 2021

Micromachines

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MicroRNAs (miRNAs) are important non-coding, single-stranded RNAs possessing crucial regulating roles in human body. Therefore, miRNAs have received extensive attention from various disciplines as the aberrant expression of miRNAs are tightly related to different types of diseases. Furthermore, the exceptional stability of miRNAs has presented them as biomarker with high specificity and sensitivity. However, small size, high sequence similarity, low abundance of miRNAs impose difficulty in their detection. Hence, it is of utmost importance to develop accurate and sensitive method for miRNA biosensing. Electrochemical biosensors have been demonstrated as promising solution for miRNA detection as they are highly sensitive, facile, and low-cost with ease of miniaturization. The incorporation of nanomaterials to electrochemical biosensor offers excellent prospects for converting biological recognition events to electronic signal for the development of biosensing platform with desired sensing properties due to their unique properties. This review introduces the signal amplification strategies employed in miRNA electrochemical biosensor and presents the feasibility of different strategies. The recent advances in nanomaterial-based electrochemical biosensor for the detection of miRNA were also discussed and summarized based on different types of miRNAs, opening new approaches in biological analysis and early disease diagnosis. Lastly, the challenges and future prospects are discussed.

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“…Among these methods, electrochemical miRNA biosensors because of superior advantages including simplicity of miniaturization, high sensitivity and selectivity, good detection limits with small analyte volumes, functional diversity, and relatively low cost have made them a suitable option for nucleic acids analysis. 12,13 This technique typically was designed based on the change in electrochemical signal which generates via the hybridization of the probe, a supplementary single-stranded nucleotide, and the target miRNA, 14 that it is suitable for low-concentration agents like miRNAs which are usually present only in femto-or nanomolar quantities in plasma. 15 Selection of the appropriate probe is one of the most important steps in electrochemical miRNA biosensors design.…”

Section: Introductionmentioning

confidence: 99%

Chemical binding of pyrrolidinyl peptide nucleic acid (acpcPNA‐T9) probe with AuNPs toward label‐free monitoring of miRNA‐21: A novel biosensing platform for biomedical analysis and POC diagnostics

Fathi

Saadati

Hasanzadeh

et al. 2021

J of Molecular Recognition

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miRNAs are attractive factors in cancer research studies due to their important roles for regulating of gene expression. Because of miRNA-21 expression surplus in many types of cancers, so accurate identification is important. Increasing efforts have caused different methods to improve the sensitivity and specificity of detection. Present study is an attempt to report a new electrochemical label-free PNA-based bioassay for detection of miRNA-21. In this study, gold electrode was modified by gold nanoparticles to improve a functional PNA-based biosensor. The EDS and field emission scanning electron microscope (FE-SEM) were used to detect fabrication of biosensor. The electrochemical behavior of sensor was evaluated after inserting of acpcPNA probes and miRNA-21 on the stucture of electrode and analyzed essential parameters such as various concentration of target miRNA, hybridization time, reproducibility, stability, and applicability. The results of study demonstrated that engineered biosensor was successfully fabricated. The findings showed the highest amount of current in 5 minutes hybridization time, with suitable reproducibility and stability. This innovative miRNA-based biosensor presents a sensitive and specific method in fast and may be lab-on chip assay in future.

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Ultrasensitive detection of microRNA based on a homogeneous label-free electrochemical platform using G-triplex/methylene blue as a signal generator

Cited by 28 publications

References 40 publications

A Homogeneous Label-Free Electrochemical microRNA Biosensor Coupling With G-Triplex/Methylene Blue Complex and λ-Exonuclease-Assisted Recycling Amplification

A Homogeneous Label-Free Electrochemical microRNA Biosensor Coupling With G-Triplex/Methylene Blue Complex and λ-Exonuclease-Assisted Recycling Amplification

Recent Progress in Nanomaterials Modified Electrochemical Biosensors for the Detection of MicroRNA

Chemical binding of pyrrolidinyl peptide nucleic acid (acpcPNA‐T9) probe with AuNPs toward label‐free monitoring of miRNA‐21: A novel biosensing platform for biomedical analysis and POC diagnostics

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