Ultrasensitive detection of gastric cancer biomarkers via a frequency shift-based SERS microfluidic chip

Abstract

Highly sensitive protein biomarker detection is critical for the diagnosis of gastric cancer (GC) whereas accurate and sensitive detection of low-abundance proteins in early-stage GC is still a challenge. Herein,...

SERS sensing for cancer biomarker: Approaches and directions

“…According to the results, t-PSA shows a dependable linear response in the concentration range of 1–200 ng/mL, but f-PSA exhibits a good linear response in the range of 0.1–20 ng/mL. In other study [ 140 ], used a customized microfluidic device intended for the detection of two gastric cancer (GC) protein biomarkers, carcinoembryonic antigen (CEA) and vascular endothelial growth factor (VEGF), to perform a SERS frequency shift experiment. The device has three parallel channel sets, each of which has two reaction areas.…”

On-Chip Photonic Detection Techniques for Non-Invasive In Situ Characterizations at the Microfluidic Scale

“…With a detection limit at the single-molecule level, SERS is considered as an ultra-sensitive technique [ 79 , 80 ]. Importantly, SERS eliminates any interference from water and does not cause damage to samples, making it suitable for the analysis of biological samples [ 81 , 82 , 83 , 84 , 85 , 86 ] or cancer diagnostics [ 87 , 88 , 89 , 90 , 91 ]. Equally, microfluidic sensors based on SERS detection have been proposed for very diverse applications, ranging from the diagnosis of Alzheimer’s disease [ 92 ] to the rapid analysis of food contaminants [ 93 ] or the high-throughput detection of explosives [ 94 ] or harmful substances [ 95 ].…”

SERS-based microdevices for use as in vitro diagnostic biosensors

“…Huang et al developed a microfluidic chip-based assay using SERS frequency shift to detect gastric cancer biomarkers CEA and VEGF. 298 The chip allows for the simultaneous analysis of multiple biomarkers in various samples. It is highly sensitive with a low detection limit.…”