Ultrasensitive detection of exosomal miRNA with PMO-graphene quantum dots-functionalized field-effect transistor biosensor

Li, Kun; Tu, Jian; Zhang, Yulin; Jin, Duo; Li, Tingxian; Li, Jiahao; Ni, Wei; Xiao, Mi; Zhang, Zhiyong; Zhang, Guojun

doi:10.1016/j.isci.2022.104522

Cited by 18 publications

(11 citation statements)

References 58 publications

(61 reference statements)

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“…Zero-dimensional nanomaterials mostly exist in spherical or quasi-spherical shapes with diameters less than 100 nm [ 47 , 64 ]. The most common 0D materials used in sensing applications are carbon QDs (CQDs) [ 65 , 66 ], semiconductor quantum dots (SQDs) [ 67 , 68 ], graphene quantum dots (GQDs) [ 62 , 69 , 70 , 71 ], CdTe QDs [ 72 , 73 , 74 ], and precious metal NPs such as silver nanoparticles (AgNPs) and AuNPs [ 18 , 20 , 75 , 76 , 77 , 78 ]. However, 0D nanomaterial-based sensing is still in its infancy, and the practical applicability of these nanosensors still has room for advancement, which needs to be solved shortly.…”

Section: Application Of 0d Nanomaterials In the Field Of Optical Fibe...mentioning

confidence: 99%

Advances in Novel Nanomaterial-Based Optical Fiber Biosensors—A Review

Singh

Wang

et al. 2022

Biosensors

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This article presents a concise summary of current advancements in novel nanomaterial-based optical fiber biosensors. The beneficial optical and biological properties of nanomaterials, such as nanoparticle size-dependent signal amplification, plasmon resonance, and charge-transfer capabilities, are widely used in biosensing applications. Due to the biocompatibility and bioreceptor combination, the nanomaterials enhance the sensitivity, limit of detection, specificity, and response time of sensing probes, as well as the signal-to-noise ratio of fiber optic biosensing platforms. This has established a practical method for improving the performance of fiber optic biosensors. With the aforementioned outstanding nanomaterial properties, the development of fiber optic biosensors has been efficiently promoted. This paper reviews the application of numerous novel nanomaterials in the field of optical fiber biosensing and provides a brief explanation of the fiber sensing mechanism.

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Section: Application Of 0d Nanomaterials In the Field Of Optical Fibe...mentioning

confidence: 99%

Advances in Novel Nanomaterial-Based Optical Fiber Biosensors—A Review

Singh

Wang

et al. 2022

Biosensors

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“…18,19 Thus, an alternative enzyme-free strategy and rapid simultaneous multiplexed detection technique for miRNAs is still urgently needed. Up to now, various amplification-free miRNA biosensors have been reported, including those based on electrochemical, 20 photoelectrochemical, 21 electrochemiluminescence, 22 field-effect transistor, 23 fluorescence, 24 surface plasmon resonance, 25 localized surface plasmon resonance, 26 waveguide interferometric, 27 and surface-enhanced Raman scattering. [28][29][30][31] The analytical performance of these biosensors is shown in Table S1.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive amplification-free detection of circulating miRNA via droplet-based processing of SERS tag–miRNA–magnetic nanoparticle sandwich nanocomplexes on a paper-based electrowetting-on-dielectric platform

Wang,

Chen,

Wang

et al. 2024

Analyst

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“…Gao et al 39 developed an ultrasensitive poly-L-lysine functionalized graphene field-effect transistor biosensor for breast cancer miRNAs detection. Li et al 40 reported the phosphorodiamidate morpholino oligomers (PMO)-graphene quantum dots (GQDs) functionalized reduced graphene oxide field effect transistor biosensors for ultrasensitive detection of exosomal miRNA21. Compared with other integrated devices, the device adopted GQDs with a three-dimensional structure, which improved the hybridization efficiency and made the FET sensor have higher sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Reduced Graphene Oxide-Based Field Effect Transistor Biosensors for High-Sensitivity miRNA21 Detection

Cai

Xia

Wang

et al. 2022

ACS Appl. Nano Mater.

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Breast cancer has been ranked the first malignant tumor in women, and with the incidence of it increasing all over the world, early screening can effectively reduce mortality and improve the cure rate. MiRNAs, as potential biomarkers, play an important role in the early diagnosis and prognosis monitoring of breast cancer. However, since miRNAs, with low concentration, are the matrix in serum or plasma, accurately detecting miRNAs without a label in serum or plasma is challenging. Herein, we proposed a biosensor combination of nanomaterial for reduced graphene oxide (RGO) and field effect transistors (FETs). The biosensor used a few layers of RGO by the chemical reduction method as a semiconductor to detect the miRNA21 in human plasma with high sensitivity (LOD: 1 fM). It can not only distinguish between perfectly matched, single-base mismatched, and completely mismatched sequences, but it can also correctly respond to clinically complex matrix samples without the process of miRNA21 extraction, amplification, and labeling. The area under the receiver operating characteristic (ROC) curve was 0.9253, which means that the biosensor can distinguish between the breast cancer patients and healthy controls and can assist in clinical diagnosis and provide new ideas for the development of clinical testing.

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Ultrasensitive detection of exosomal miRNA with PMO-graphene quantum dots-functionalized field-effect transistor biosensor

Cited by 18 publications

References 58 publications

Advances in Novel Nanomaterial-Based Optical Fiber Biosensors—A Review

Advances in Novel Nanomaterial-Based Optical Fiber Biosensors—A Review

Ultrasensitive amplification-free detection of circulating miRNA via droplet-based processing of SERS tag–miRNA–magnetic nanoparticle sandwich nanocomplexes on a paper-based electrowetting-on-dielectric platform

Reduced Graphene Oxide-Based Field Effect Transistor Biosensors for High-Sensitivity miRNA21 Detection

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