Ultrasensitive Aptasensor for Microcystin-LR Detection in Food Samples Based on Target-Activated Assembly of Y-Shaped Hairpin Probes

Shi, Gu; Yan, Chong; Chen, Junhua

doi:10.1021/acs.jafc.2c07661

Cited by 5 publications

(4 citation statements)

References 39 publications

(50 reference statements)

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“…Therefore, biosensors are powerful tools that enable rapid detection . Among them, single-stranded DNA/RNA sequences with high specificity and affinity are widely known as aptamers, and a large number of biosensors have been rationally designed based on aptamers. , Aptamers have the advantages of bulk machine synthesis, lower cost, less variation in activity between batches, and ease of modification and immobilization. − However, despite the advantages of nucleic acid aptamers, screening to identify aptamers with affinity for the target can be time-consuming due to the challenges associated with the SELEX process. , Furthermore, the binding affinity between heavy metal ion aptamers and the targets does not usually meet current requirements. , …”

Section: Introductionmentioning

confidence: 99%

Detection of Cadmium(II) in Aquatic Products Using a Rolling-Circle Amplification-Coupled Ratio Fluorescent Probe Based on an Aptamer–Peptide Conjugate

Peng,

Sha,

Fang

et al. 2024

J. Agric. Food Chem.

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The existing aptamers for cadmium (Cd 2+ ), the common toxic heavy metal contaminant in food, cannot meet the requirements for detecting Cd 2+ in rapid detection methods. In previous work, we found that coupling aptamer−peptide conjugates (APCs) with peptides and aptamers can provide a less disruptive method with a significantly improved affinity. Moreover, we found that the spatial conformation of aptamers and peptides is crucial for obtaining proper affinity in APC. Therefore, we describe a simple design strategy to obtain a series of APCs with different affinities by designing peptide orientations (N-terminal, C-terminal). The best affinity was found for APC(C1−N) with a binding constant (K a ) of 2.23 × 10 6 M −1 , indicating that the APC(C1−N) affinity was significantly increased by 829.17% over aptamer. Finally, a rolling-circle amplification (RCA)-coupled ratio fluorescencebased biosensor for Cd 2+ detection was established with a detection limit of 0.0036 nM, which has great potential for practical aquatic product detection.

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Section: Introductionmentioning

confidence: 99%

Detection of Cadmium(II) in Aquatic Products Using a Rolling-Circle Amplification-Coupled Ratio Fluorescent Probe Based on an Aptamer–Peptide Conjugate

Peng,

Sha,

Fang

et al. 2024

J. Agric. Food Chem.

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“…High signal amplification sensors based on DNA self-assembly have been widely used in miRNA detection. 24 In addition, the detection platform of toxins, 25 antibiotics 26 and bacteria, 27 which combines catalytic hairpin self-assembly with aptamer technology, has also been widely reported.…”

Section: Introductionmentioning

confidence: 99%

A enzyme-free fluorescence quenching sensor for amplified detection of kanamycin in milk based on competitive triggering strategies

Bao,

Sang,

Yan

et al. 2024

RSC Adv.

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“…Current methods for the detection and quantification of TCs are mainly based on the combination of liquid chromatography with other methods (e.g., mass spectrometry, and UV detection) and capillary electrophoresis . These analytical methods require large instruments and therefore do not allow for on-site screening tests .…”

Section: Introductionmentioning

confidence: 99%

Broad-Spectrum Detection of Tetracyclines by Riboswitch-Based Cell-Free Expression Biosensing

Cheng

Qin

et al. 2023

J. Agric. Food Chem.

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We describe a sensitive and selective method for the determination of tetracycline content in foods using a riboswitch sensor. The sensor is based on a cell-free expression system that can be lyophilized to produce paper-based sensors or tube-based sensors for long-term storage. The riboswitch constructed using artificially screened tetracycline RNA aptamers was cloned into the pET-28a(+) vector of Escherichia coli TOP 10. The expression of the green fluorescent protein was positively correlated with the concentration of tetracyclines. The binding of tetracyclines to the aptamer domain results in a conformational change in the riboswitch secondary structure, resulting in the exposure of the ribosome binding site thereby promoting expression. The detection limits of the prepared sensor for the detection of tetracycline, oxytetracycline, chlortetracycline, and doxycycline were 0.47, 0.079, 0.084, and 0.43 μM, respectively. Moreover, the 1 μM tetracyclines allow for qualitative detection in milk samples by the naked eye. The work provides a proof-of-principle for riboswitch design to address global health and food safety.

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Ultrasensitive Aptasensor for Microcystin-LR Detection in Food Samples Based on Target-Activated Assembly of Y-Shaped Hairpin Probes

Cited by 5 publications

References 39 publications

Detection of Cadmium(II) in Aquatic Products Using a Rolling-Circle Amplification-Coupled Ratio Fluorescent Probe Based on an Aptamer–Peptide Conjugate

Detection of Cadmium(II) in Aquatic Products Using a Rolling-Circle Amplification-Coupled Ratio Fluorescent Probe Based on an Aptamer–Peptide Conjugate

A enzyme-free fluorescence quenching sensor for amplified detection of kanamycin in milk based on competitive triggering strategies

Broad-Spectrum Detection of Tetracyclines by Riboswitch-Based Cell-Free Expression Biosensing

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