Ultrasensitive and ratiometric two-photon fluorescence imaging of Golgi polarity during drug-induced acute kidney injury

Abstract

We synthesized the ultrasensitive probe TP-Golgi for the two-photon ratiometric fluorescence imaging of Golgi polarity. The probe TP-Golgi possesses a large Stokes shift, excellent sensitivity and good selectivity to quantitively...

Golgi-Targeted Fluorescent Probe for Imaging NO in Alzheimer’s Disease

“…Fluorescent probes are widely used for in situ sensing bioactive species in cells because they are noninvasive, are easy to handle, and can reveal the subcellular spatial heterogeneity of the targets. − So far, several fluorescent probes for imaging cellular NO have been reported including the commercially available diaminofluorescein diacetate (DAF-DA), most of which are based on the NO-induced oxidation of o -diaminobenzene. − However, these probes cannot image NO in the Golgi apparatus due to the lack of subcellular targeting ability. In 2019, 4-sulfamoylphenylamide was proposed as a simple and effective Golgi-targeted moiety for constructing an H 2 O 2 fluorescent probe; afterward, other researchers also proved the effectiveness of this moiety for targeting the Golgi apparatus. , In the present study, therefore, we envisage that 4-sulfamoylphenylamide may also be employed to design a Golgi-targeted fluorescent probe for NO detection. In our previous work, 4-sulfamoylphenylamide was grafted to the 2-carboxyl of rhodamine B, and the resulting product was used to demonstrate its specificity for the Golgi apparatus .…”

“…In 2019, 4-sulfamoylphenylamide was proposed as a simple and effective Golgi-targeted moiety for constructing an H 2 O 2 fluorescent probe; 25 afterward, other researchers also proved the effectiveness of this moiety for targeting the Golgi apparatus. 26,27 In the present study, therefore, we envisage that 4-sulfamoylphenylamide may also be employed to design a Golgi-targeted fluorescent probe for NO detection. In our previous work, 4-sulfamoylphenylamide was grafted to the 2-carboxyl of rhodamine B, and the resulting product was used to demonstrate its specificity for the Golgi apparatus.…”

Polarity-Sensitive and Membrane-Specific Probe Quantitatively Monitoring Ferroptosis through Fluorescence Lifetime Imaging

“…Lipid peroxidation pronouncedly changes the membranes’ compositions and structures, inevitably leading to changes in the local micro-environmental factors, especially polarity. Actually, polarity variations in different intracellular areas had been recognized as reliable indictors of different diseases. − Among all cell membranes, the plasma membrane serving as the boundary to separate, and the interface to connect the internal and external environments of the cell, , is not only important, but also relatively easy for visualization. Therefore, it is a recommendable strategy to monitor the polarity of plasma membranes, as a convenient way to indicate ferroptosis extents, which will find important implications for the diagnosis and research of related diseases.…”

An Integrated Fluorescent Probe for Ratiometric Detection of Glutathione in the Golgi Apparatus and Activated Organelle-Targeted Therapy

“…Subsequently, based on the same theory, many other outstanding works have also been developed for the treatment of tumors. − Thus, the release of drugs through overexpression of GSH in tumor cells provides a very excellent approach for the treatment of cancer. To further improve the therapeutic effect, some subcellular organelles are wonderful treated targets, − since they have a high relationship with a variety of cellular activities and act an extremely major part in manipulating cellular status. − Obviously, the development of molecules that target organelles and control the release of drugs by overexpressing GSH in tumor cells is of great significance for cancer treatment.…”