Ultrasensitive and label-free electrochemical aptasensor based on carbon dots-black phosphorus nanohybrid for the detection of Ochratoxins A

Li, Kunxia; Qiao, Xiujuan; Zhao, Haiyan; He, Yaping; Sheng, Qinglin; Yue, Tianli

doi:10.1016/j.microc.2021.106378

Cited by 20 publications

(6 citation statements)

References 42 publications

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“…When compared with previously reported determination methods for OTA ,,,− (Table S1), the proposed dual-channel assay was a comparable or a more favorable assay with a wider linear range and a lower detection limit, indicating the superior sensing performance of the method. The sensitivity was much lower than the standard residue levels regulated by EC1881–2006 (5 μg/kg, corresponding to 10 pg/mL), suggesting that this method has substantial potential for OTA detection in real samples.…”

Section: Resultsmentioning

confidence: 84%

Simple Design Concept for Dual-Channel Detection of Ochratoxin A Based on Bifunctional Metal–Organic Framework

Zhang

et al. 2022

ACS Appl. Mater. Interfaces

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A simple fluorescence and electrochemical dualchannel biosensor based on bifunctional Zr(IV)-based metal− organic framework (Zr-MOF) was proposed to detect Ochratoxin A (OTA). The bifunctional Zr-MOF, with photoluminescence properties and enormous electroactive ligands, was exploited to load OTA-specific aptamers for designing signal probes, greatly simplifying the probe-fabrication process and improving sensing reliability. Upon specific recognition of aptamer toward OTA, the anchored probe was released from the sensing interface into the reaction solution. In this circumstance, the increased amount of the signal probe in reaction solution led to an enhanced fluorescence response, while the decreased amount of the signal probe on the sensing interface resulted in a diminished electrochemical response. According to the dual-channel signal change with increasing OTA concentration, the visual fluorescence strategy was established for intuitive OTA detection, and meanwhile, sensitive electrochemical assay with a detection limit of 0.024 pg/mL was also achieved with the help of one-step electrodeposition as a sensing platform. Moreover, the proposed dual-channel assay has been successfully applied to determine OTA levels in corn samples with rapid response, superior accuracy, and high antiinterference capability, providing a promising method for food safety monitoring.

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Section: Resultsmentioning

confidence: 84%

Simple Design Concept for Dual-Channel Detection of Ochratoxin A Based on Bifunctional Metal–Organic Framework

Zhang

et al. 2022

ACS Appl. Mater. Interfaces

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“…High-resolution XPS spectra of the P 2p signal enable a precise investigation of the oxidation state of P. In the P 2p spectra of BPNPs ( Figure 3 C), there were two peaks at 130.2 eV and 131.0 eV, corresponding to single spin-orbit P–P 2p 3/2 (green) and P–P 2p 1/2 (purple), respectively [ 41 ]. The third relatively weak peak at 134.0 eV was observed by slight oxidation of BPNPs, which was attributed to P–O bonds (brown) [ 42 ]. In the spectrum of BPQDs ( Figure 3 D), the intensity of P–O bonding of BPQDs was higher than of BPNPs, indicating that the electron of P was taken by O in the process of exfoliated BPQDs.…”

Section: Resultsmentioning

confidence: 99%

Electrochemical Biosensor Based on Horseradish Peroxidase and Black Phosphorene Quantum Dot Modified Electrode

Li,

Shi,

Wang

et al. 2023

Molecules

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Black phosphorene quantum dots (BPQDs) were prepared by ultrasonic-assisted liquid-phase exfoliation and centrifugation with morphologies proved by TEM results. Furthermore, an electrochemical enzyme sensor was prepared by co-modification of BPQDs with horseradish peroxidase (HRP) on the surface of a carbon ionic liquid electrode (CILE) for the first time. The direct electrochemical behavior of HRP was studied with a pair of well-shaped voltammetric peaks that appeared, indicating that the existence of BPQDs was beneficial to accelerate the electron transfer rate between HRP and the electrode surface. This was due to the excellent properties of BPQDs, such as small particle size, high interfacial reaction activity, fast conductivity, and good biocompatibility. The presence of BPQDs on the electrode surface provided a fast channel for direct electron transfer of HRP. Therefore, the constructed electrochemical HRP biosensor was firstly used to investigate the electrocatalytic behavior of trichloroacetic acid (TCA) and potassium bromate (KBrO3), and the wide linear detection ranges of TCA and KBrO3 were 4.0–600.0 mmol/L and 2.0–57.0 mmol/L, respectively. The modified electrode was applied to the actual samples detection with satisfactory results.

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“…Later, they were replaced with less toxic and more effective organophosphorus (OPs) pesticides due to their hazardous consequences. OPs were formerly thought to be a safe alternative to OCs, but their widespread usage, accumulation, and exposure have caused severe toxicological consequences on organisms that are not targeted [ 105 , 106 , 107 ]. OP compound poisoning is a global health issue, causing over three million poisonings and 200 thousand fatalities yearly [ 13 , 108 , 109 , 110 ].…”

Section: Biomimetic Nanosensors For the Detection Of Pesticidesmentioning

confidence: 99%

Progress on Electrochemical Biomimetic Nanosensors for the Detection and Monitoring of Mycotoxins and Pesticides

Lakavath,

Kafley,

Sajeevan

et al. 2024

Toxins

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Monitoring agricultural toxins such as mycotoxins is crucial for a healthy society. High concentrations of these toxins lead to the cause of several chronic diseases; therefore, developing analytical systems for detecting/monitoring agricultural toxins is essential. These toxins are found in crops such as vegetables, fruits, food, and beverage products. Currently, screening of these toxins is mostly performed with sophisticated instrumentation such as chromatography and spectroscopy techniques. However, these techniques are very expensive and require extensive maintenance, and their availability is limited to metro cities only. Alternatively, electrochemical biomimetic sensing methodologies have progressed hugely during the last decade due to their unique advantages like point-of-care sensing, miniaturized instrumentations, and mobile/personalized monitoring systems. Specifically, affinity-based sensing strategies including immunosensors, aptasensors, and molecular imprinted polymers offer tremendous sensitivity, selectivity, and stability to the sensing system. The current review discusses the principal mechanisms and the recent developments in affinity-based sensing methodologies for the detection and continuous monitoring of mycotoxins and pesticides. The core discussion has mainly focused on the fabrication protocols, advantages, and disadvantages of affinity-based sensing systems and different exploited electrochemical transduction techniques.

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Ultrasensitive and label-free electrochemical aptasensor based on carbon dots-black phosphorus nanohybrid for the detection of Ochratoxins A

Cited by 20 publications

References 42 publications

Simple Design Concept for Dual-Channel Detection of Ochratoxin A Based on Bifunctional Metal–Organic Framework

Simple Design Concept for Dual-Channel Detection of Ochratoxin A Based on Bifunctional Metal–Organic Framework

Electrochemical Biosensor Based on Horseradish Peroxidase and Black Phosphorene Quantum Dot Modified Electrode

Progress on Electrochemical Biomimetic Nanosensors for the Detection and Monitoring of Mycotoxins and Pesticides

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