Ultrasensitive and label-free biosensor for the detection of Plasmodium falciparum histidine-rich protein II in saliva

Soraya, Gita Vita; Abeyrathne, Chathurika D.; Buffet, Christelle; Huynh, Duc; Uddin, Shah Mukim; Chan, Jianxiong; Skafidas, Efstratios; Kwan, Patrick; Rogerson, Stephen J.

doi:10.1038/s41598-019-53852-5

Cited by 20 publications

(9 citation statements)

References 40 publications

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“…Ideally, such challenges and limitations can potentially be overcome with properly designed biosensors, that must fill the gap of high sensitivity and specificity, as well as being easily miniaturized for point-of-care diagnosis. Although there are several reports of biosensors for malaria diagnosis, these are mainly based on the use of enzymes as a target [ 11 , 16 , 17 , 52 , 53 , 54 ], i.e., RDTs. Nevertheless, as mentioned above, these have been threatened by parasite genetic evolution, and they do not detect low-parasitemia (100 parasites/µL of blood far from the ideal less than 5 parasites/µL of blood) or quantify infection.…”

Section: Biosensors For Hemozoin-based Malaria Diagnosismentioning

confidence: 99%

Review of Microdevices for Hemozoin-Based Malaria Detection

et al. 2022

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Despite being preventable and treatable, malaria still puts almost half of the world’s population at risk. Thus, prompt, accurate and sensitive malaria diagnosis is crucial for disease control and elimination. Optical microscopy and immuno-rapid tests are the standard malaria diagnostic methods in the field. However, these are time-consuming and fail to detect low-level parasitemia. Biosensors and lab-on-a-chip devices, as reported to different applications, usually offer high sensitivity, specificity, and ease of use at the point of care. Thus, these can be explored as an alternative for malaria diagnosis. Alongside malaria infection inside the human red blood cells, parasites consume host hemoglobin generating the hemozoin crystal as a by-product. Hemozoin is produced in all parasite species either in symptomatic and asymptomatic individuals. Furthermore, hemozoin crystals are produced as the parasites invade the red blood cells and their content relates to disease progression. Hemozoin is, therefore, a unique indicator of infection, being used as a malaria biomarker. Herein, the so-far developed biosensors and lab-on-a-chip devices aiming for malaria detection by targeting hemozoin as a biomarker are reviewed and discussed to fulfil all the medical demands for malaria management towards elimination.

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Section: Biosensors For Hemozoin-based Malaria Diagnosismentioning

confidence: 99%

Review of Microdevices for Hemozoin-Based Malaria Detection

et al. 2022

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“…In healthcare, a great deal of biosensors have been developed to detect pathogens and cancers, as well as to measure glucose and oxygen levels. Impedimetric biosensors gain traction in diagnostic purposes due to their high sensitivity, low cost and miniaturization capability [ 64 ]. For malaria diagnosis, various types of immunosensors for detection of Pf HRP2 were fabricated on the basis of direct or sandwich immunoassays, with detection limit as low as 10 fg/mL in spiked plasma and 18 fg/mL in spiked whole blood [ 65 ].…”

Section: Antigen-based Diagnostic Methodsmentioning

confidence: 99%

Urine and Saliva: Relevant Specimens for Malaria Diagnosis?

Chai

Chua

2022

Diagnostics

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Blood remains the specimen of preference for malaria diagnosis, whether it is for microscopic, nucleic acid-based or biomarker detection of Plasmodium present in a patient. However, concerning the disadvantages of blood drawing, specimens that can be non-invasively collected under non-hygienic settings would come in handy for malaria diagnosis in endemic areas with limited resources. Although the current approaches using saliva or urine might not be as sensitive and specific as using blood, the potential of these two specimens should not be underestimated and efforts in developing diagnostic methods for Plasmodium detection specifically in these two specimens should continue without giving up. This review not only compiles and summarizes the sensitivity and specificity achieved by various detection approaches when using these samples for malaria diagnosis, it also intends to enhance the possibility of using saliva and urine for diagnostic purposes by describing how Plasmodium nucleic acid and antigens may likely be present in these samples. This review may hopefully encourage and motivate researchers in developing saliva- and urine-based diagnostic methods for Plasmodium detection to facilitate the control and eradication of malaria. In summary, the presence of Plasmodium DNA and antigens in urine and saliva makes these two specimens relevant and useful for malaria diagnosis.

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“…As a non-destructive technique, EIS was used to monitor all the stages involved and it enabled rapid detection of the disease. Indeed the work done by Soraya et al [ 76 ] reported the development of an ultrasensitive and label free sensor for histidine-rich protein 2 produced by the malaria parasite Plasmodium falciparum (fpHRP2) and the sensor showed enough potential to be developed into a POC diagnostic device for eliminating malaria. The impedance sensor demonstrated the use of an interdigitated electrode sensor surface for immobilizing the capture probe anti- fpHRP2 monoclonal antibodies.…”

Section: Review Of Recent Electrochemical Biosensors For Eids and Re-mentioning

confidence: 99%

Recent advances and challenges in electrochemical biosensors for emerging and re-emerging infectious diseases

Menon

Mathew

Sam

et al. 2020

Journal of Electroanalytical Chemistry

137

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The rise of emerging infectious diseases (EIDs) as well as the increase in spread of existing infections is threatening global economies and human lives, with several countries still fighting repeated onslaught of a few of these epidemics. The catastrophic impact a pandemic has on humans and economy should serve as a reminder to be better prepared to the advent of known and unknown pathogens in the future. The goal of having a set of initiatives and procedures to tackle them is the need of the hour. Rapid detection and point-of-care (POC) analysis of pathogens causing these diseases is not only a problem entailing the scientific community but also raises challenges in tailoring appropriate treatment strategies to the healthcare sector. Among the various methods used to detect pathogens, Electrochemical Biosensor Technology is at the forefront in the development of POC devices. Electrochemical Biosensors stand in good stead due to their rapid response, high sensitivity and selectivity and ease of miniaturization to name a few advantages. This review explores the innovations in electrochemical biosensing based on the various electroanalytical techniques including voltammetry, impedance, amperometry and potentiometry and discusses their potential in diagnosis of emerging and re-emerging infectious diseases (Re-EIDs), which are potential pandemic threats.

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Ultrasensitive and label-free biosensor for the detection of Plasmodium falciparum histidine-rich protein II in saliva

Cited by 20 publications

References 40 publications

Review of Microdevices for Hemozoin-Based Malaria Detection

Review of Microdevices for Hemozoin-Based Malaria Detection

Urine and Saliva: Relevant Specimens for Malaria Diagnosis?

Recent advances and challenges in electrochemical biosensors for emerging and re-emerging infectious diseases

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