Ultraperformance liquid chromatography tandem mass spectrometric method for direct quantification of salbutamol in urine samples in doping control

Ventura, Rosa; Ramirez, R D; Monfort, Núria; Segura, Jordi

doi:10.1016/j.jpba.2009.06.009

Cited by 25 publications

(20 citation statements)

References 17 publications

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“…In recent years, several methods have been developed using ultra-high performance liquid chromatography (UHPLC) to improve separation of multi-class analytes in short run times (Aguilera-Luiz, Vidal, Romero-González, & Frenich, 2008;Frenich, Vidal, Romero-González, & Aguilera-Luiz, 2009;Peters, Bolck, Rutgers, Stolker, & Nielen, 2009;Ventura, Ramírez, Monfort, & Segura, 2009). Furthermore, the emergence of stable detector power sources allows the fast polarity switching with minimal delays as low as 20 ms to enable detection of both positively and negatively charged compounds in a single injection (Whelan et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of nitroimidazoles, benzimidazoles, and chloramphenicol components in bovine milk by ultra-high performance liquid chromatography–tandem mass spectrometry

Wang

Zhang

et al. 2016

Food Chemistry

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Section: Introductionmentioning

confidence: 99%

Simultaneous determination of nitroimidazoles, benzimidazoles, and chloramphenicol components in bovine milk by ultra-high performance liquid chromatography–tandem mass spectrometry

Wang

Zhang

et al. 2016

Food Chemistry

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“…A variety of analytical methods such as gas chromatography (Caban et al 2011;Eenoo and Delbeke 2002;Forsdahl and Gmeiner 2004), capillary electrophoresismass spectrometry (Anurukvorakun et al 2010;Hung et al 2010), and highperformance liquid chromatography-tandem mass spectrometry (HPLC-MS-MS) (Ventura et al 2009;Garcia et al 2011) have been applied for the determination of salbutamol. However, these instrumental methods require time-consuming sample preparation and sophisticated technicians.…”

Section: Introductonmentioning

confidence: 99%

Determination of Salbutamol, Clenbuterol, and Brombuterol in Urine by a Highly Sensitive Chemiluminescence Enzyme Immunoassay

Jiang

et al. 2014

Analytical Letters

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A salbutamol-bovine serum albumin and a salbutamol-ovalbumin coating antigen were synthesized, and six New Zealand white rabbits were treated with the immunogen to obtain polyclonal antibodies to develop a rapid, sensitive, and indirect chemiluminescent enzyme immunoassay (ciCLEIA) for the analysis of swine and bovine urine. The prepared antibodies showed high cross-reactivities with clenbuterol (139.6%) and brombuterol (225%). Under the optimized conditions, the linear dynamic range and the limit of detection (LOD) for salbutamol were from 0.007 to 0.17 lg L À1 and 0.003 lg L À1 , with a correlation coefficient of 0.9965 and a half maximum inhibition concentration of 0.028 lg L À1 . Recoveries for salbutamol, clenbuterol, and brombuterol were from 78.8% to 119.0% with intra-assay and inter-assay coefficients of variation less than 13.9% and 19.7%, respectively. The reported ciCLEIA was about 10-fold more sensitive for salbutamol and 20-fold more sensitive for clenbuterol compared to conventional methods. This study showed that ciCLEIA was a reliable, convenient, and sensitive method for the simultaneous determination of salbutamol, clenbuterol, and brombuterol in swine and bovine urine.

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“…Different methods including high-performance liquid chromatography (HPLC) [4] and capillary electrochromatography (CEC) [5] have been applied for the enantiomeric separation of SAL. SAL has been determined in biological media using LC-MS [6], CE [2] and HPLC [7].…”

Section: Introductionmentioning

confidence: 99%

High performance liquid chromatographic determination of some guaiphenesin-containing cough-cold preparations

Korany

Fahmy

Mahgoub

et al. 2011

Journal of Advanced Research

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This paper presents different HPLC methods for the simultaneous determination of some guaiphenesin-containing cough-cold preparations. Three pharmaceutically available combinations were analyzed: salbutamol sulfate (SAL) and guaiphenesin (GUA), combination I; ascorbic acid (ASC), paracetamol (PAR) and guaiphenesin (GUA), combination II; and theophylline anhydrous (THE), guaiphenesin (GUA) and ambroxol hydrochloride (AMB), combination III. A 250 · 4.6 mm C-18 column was used for all combinations. The mobile phase for the three combinations consisted of a mixture of methanol and 0.01 M aqueous phosphate buffer solution. The pH of the mobile phase was adjusted to 3.2, 6.2 and 3.8 for combinations I, II and III, respectively. The proposed HPLC methods were successfully applied to the determination of the investigated drugs, both in synthetic mixtures and in pharmaceutical preparations, without any matrix interference and with high precision and accuracy. Different aspects of analytical validation are presented in the text.

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Ultraperformance liquid chromatography tandem mass spectrometric method for direct quantification of salbutamol in urine samples in doping control

Cited by 25 publications

References 17 publications

Simultaneous determination of nitroimidazoles, benzimidazoles, and chloramphenicol components in bovine milk by ultra-high performance liquid chromatography–tandem mass spectrometry

Simultaneous determination of nitroimidazoles, benzimidazoles, and chloramphenicol components in bovine milk by ultra-high performance liquid chromatography–tandem mass spectrometry

Determination of Salbutamol, Clenbuterol, and Brombuterol in Urine by a Highly Sensitive Chemiluminescence Enzyme Immunoassay

High performance liquid chromatographic determination of some guaiphenesin-containing cough-cold preparations

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