Ultramicroscopic Erythrocytes Profile as a Component of the Babesiosis Pathogenesis

Torianyk, Inna

doi:10.36740/wlek202104119

Cited by 2 publications

(1 citation statement)

References 14 publications

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“…Задля об'єктивізації дослідження результати гістологічного аналізу верифікували цитологічно (у мазках периферичної крові [13] ресурсами растрової електронної мікроскопії [14], молекулярно-генетичного методу (ПЛР) [10], підтверджували в експерименті за результатами біологічних проб [15]).…”

Section: матеріали та методи дослідженняunclassified

Гістологічний Аналіз Лімфоїдних Утворень Шлунка І Тонкої Кишки У Клініко-Патогенетичному Профілі Бабезіозу

Торяник¹

2021

Med. and Ecol. probl.

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Peripheral lymphoid organs respond immediately to a decrease in the body's defence responses, and their localisation and morphological specificity account for their active involvement in immunogenesis (especially in animals). This fact accentuates the morpho-functional estimation of the lymphoid tissue condition with the purpose of timely diagnostics of starting deviations and preventing the development of decompensated changes. Thus, the analysis of histological changes of the latter in natural and experimental prototypes of babesiosis is relevant. The aim of the research is to define the role of histological changes of lymphoid masses in the stomach and small intestine in the formation of the clinical and pathogenetic profile of babesiosis. We studied the structural changes of lymphoid masses of the stomach and small intestine in animals with babesiosis postmortem by histological methods. Fixation was traditionally carried out in 12% aqueous formalin solution, dehydration in alcohol (30º to absolute), and pouring (paraffin/ celloidin). Staining was conducted with eosin and haematoxylin, Van Gizon, Brasche. Analysis was performed using an Olympus BX-41 microscope, Japan (x 100; x 200; x 1350). Verification was carried out by cytological, ultramicroscopic, molecular genetic methods (polymerase chain reaction) and in a biological experiment on animals. Analysis of specimens of the lamina propria in the gastric mucosa of the animals in the experimental group and clinical observation group established that the latter contained diffuse accumulations of the lymphoid tissue. Their dispersal involved different parts of the organ, including quite large areas. Haematoxylin and eosin staining gave a clear histological pattern with a marked contrast of fragments, which contributed to diagnostic resources. Lymphoid follicles were observed occasionally, their marginal, mantle zones were blurred. The germinative centres were not detected and did not show any lucency. Neutrophilic granulocytes, monocytes and plasma cells predominated among the cellular leucocyte populations. Examination of the lymphoid apparatus of the small intestine showed predominantly diffuse nodular hyperplasia, which was widespread. A focal version of the nosological prototype was observed in individual specimens occasionally, presented as single cells (most often in the terminal parts of the organ). Histological changes of lymphoid masses of the stomach and small intestine in animals with babesiosis consisted in the development of expressive diffuse nodular hyperplasia with disorganization of the structure of lymphoid follicles, destruction of zonality of the latter, making it impossible to detect germinative centres.

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Section: матеріали та методи дослідженняunclassified

Гістологічний Аналіз Лімфоїдних Утворень Шлунка І Тонкої Кишки У Клініко-Патогенетичному Профілі Бабезіозу

Торяник¹

2021

Med. and Ecol. probl.

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Ultramicroscopic Characteristics of Erythrocytes in Individuals With Borreliosis, Rheumatoid Arthritis and Toxoplasmosis as a Marker, Comorbid or Concomitant Pathology for Babesiosis

Torianyk¹

2021

Wiad Lek

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The aim is to determine the ultramicroscopic characteristics of erythrocytes in individuals with rheumatoid arthritis, borreliosis or toxoplasmosis as a marker, comorbid or concomitant pathology for babesiosis. Materials and methods: Blood samples from the patients with revealed borreliosis (Lyme disease) (19 cases), toxoplasmosis (15 cases), rheumatoid arthritis in the stage ofexacerbation (10 cases) served as the study material (group 2). In all patients of group 2, positive results for babesiosis were obtained during the polymerase chain reaction. The group of comparative control (group 1) consisted of clinically healthy people (n=31), who underwent the blood cytological examination (light microscopy) preceding the scanning electron microscopy, followed by verification of the results by resources of molecular genetic research (polymerase chain reaction). Scanning electron microscopy was used in this study. Results: In patients with babesiosis and marker, comorbid and concomitant conditions for this disease (rheumatoid arthritis, borreliosis, toxoplasmosis) it was identified the specific diagnostic criteria for the presence of extraerythrocyte forms of babesia, constant number of erythrocytes and their regenerative forms, the appearance of degenerative forms of erythrocytes with their size and shape pathology. The latter lead to hemodynamic disorders, the development of ischemic and hypoxic changes in tissues of different organs of human body. Conclusions: Scanning electron microscopy of erythrocytes in patients with babesiosis and marker, comorbid and concomitant conditions for this disease (rheumatoid arthritis, borreliosis, toxoplasmosis) plays the role of an objective method of verifying the results of previous clinical and laboratory diagnosis. The use of scanning electron microscopy allow us to determine in these patients the specific diagnostic criteria for the presence of extraerythrocyte forms of babesia, constant number of erythrocytes and their regenerative forms, degenerative forms of erythrocytes with their size and shape pathology.

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Ultramicroscopic Erythrocytes Profile as a Component of the Babesiosis Pathogenesis

Cited by 2 publications

References 14 publications

Гістологічний Аналіз Лімфоїдних Утворень Шлунка І Тонкої Кишки У Клініко-Патогенетичному Профілі Бабезіозу

Гістологічний Аналіз Лімфоїдних Утворень Шлунка І Тонкої Кишки У Клініко-Патогенетичному Профілі Бабезіозу

Ultramicroscopic Characteristics of Erythrocytes in Individuals With Borreliosis, Rheumatoid Arthritis and Toxoplasmosis as a Marker, Comorbid or Concomitant Pathology for Babesiosis

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