Ultralow Ru Single Atoms Confined in Cerium Oxide Nanoglues for Highly‐Sensitive and Robust H₂O₂‐Related Biocatalytic Diagnosis

Abstract

Exploring highly efficient, portable, and robust biocatalysts is a great challenge in colorimetric biosensors. To overcome the challenging states in creating single‐atom biocatalysts, such as insufficient activity and stability, here, this work has engineered a unique CeO2 support as nanoglue to tightly anchor the Ru single‐atom sites (CeO2‐Ru) with strong electronic coupling for achieving highly sensitive and robust H2O2‐related biocatalytic diagnosis. The morphology and chemical/electronic structure analysis demonstrates that the Ru atoms are well‐dispersed on CeO2 surface to form high‐density active sites. Benefiting from the unique structure, the prepared CeO2‐Ru exhibits outstanding peroxidase (POD) like catalytic activity and selectivity to H2O2. Steady‐state kinetic study results show that the CeO2‐Ru presents the highest Vmax and turnover number than the state‐of‐the‐art POD‐like biocatalysts. Consequently, the CeO2‐Ru discloses a high efficiency, good selectivity, and robust stability in the colorimetric detection of L‐cysteine, glucose, and uric acid. Notably, the limit of detection (LOD) can reach 0.176 × 10−3 m for the L‐cysteine, 0.095 × 10−3 m for the glucose, and 0.088 × 10−3 m for the uric acid via cascade reaction. This work suggests that the proposed unique CeO2 nanoglue will offer a new path to create single‐atom noble metal biocatalysts and take a step closer to future biotherapeutic and biocatalytic applications.

Spatiotemporally Controllable Bifunctional Micellar-Carbon Dot Supernanoparticles for Wounds Healing

“…The full XPS spectra reveal that the Ce-CDs were composed of five elements, including C, O, N, S, and Ce, thus proving the successful doping of Ce, N, S (Figure S2A). Additionally, the high-resolution spectrum of Ce 3d was divided into nine peaks (Figure E), with the peaks located at 904.1, 899.1, 885.4, 881.5 eV corresponding to Ce (III), and the remaining peaks representing Ce (IV), indicating the coexistence of Ce (IV) and Ce (III) on the surface of Ce-CDs. , The N 1s spectra exhibit two distinct peaks at 400.2 and 402.7 eV, attributed to pyrrolic-N and graphitic-N, respectively (Figure S2B). , The high-resolution C 1s spectra can be divided into three major peaks (Figure S2C), with the peak at 284.9 eV assigned to the CC structure, the peak at 286.5 eV indicating the presence of the C–N/C–O/C–S structure, and the peak at 288.7 eV attributed to the CO structure. , …”

In Situ Electrospinning MOF-Derived Highly Dispersed α-Cobalt Confined in Nitrogen-Doped Carbon Nanofibers Nanozyme for Biomolecule Monitoring

“…The rapid and accurate detection of biomolecules is crucial to diagnose, track, and prevent varieties of diseases. − Recently, the colorimetric assay has received considerable attention for detecting different biological molecules owing to its easy operation, high efficiency, and “naked eye” visual detection. − It is worth mentioning that nanozymes have been widely studied as substitutes for natural enzymes in colorimetric assays, which can be attributed to the high enzyme-like activity, easy preparation, and strong stability in extreme conditions of nanozymes. − However, developing nanozymes with highly dispersed active sites and high enzyme-mimicking catalytic activities as well as robust structures for colorimetric biosensing diverse biomolecules still remains a substantial challenge. Metal–organic frameworks (MOFs) are porous organic–inorganic coordination materials with a large number of active sites that have been utilized as nanozymes in the fields of biotherapy, antibacterial, biosensors, and pollutant degradation. , Recently, Co nanoparticles derived from MOF with excellent enzyme-like activities have attracted considerable attention in many fields .…”