Ultralow-aspect-ratio micropore sensor for a particle translocation: Critical role of the access region

Ryu, Jihun; Choi, Wonhyung; Jeon, Eun Seok; Song, Chang Woo; Han, Chang Soo

doi:10.1016/j.snb.2019.02.090

Cited by 7 publications

(2 citation statements)

References 29 publications

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“…Solid state pores, from nano-to micrometric dimensions, have been extensively used over the last decade for sensing biomolecules and cells. [1][2][3] After application of a DC electric field between two electrodes present at each side of the pore, the passage of biomolecules through the pore is detected by a change membrane before entering the pore, finally resulting in loss of sensitivity with this method. [14] Several methods have been developed that permit a localized functionalization of the pore while excluding the surrounding membrane.…”

Section: Introductionmentioning

confidence: 99%

“…Solid state pores, from nano‐ to micrometric dimensions, have been extensively used over the last decade for sensing biomolecules and cells. [ 1–3 ] After application of a DC electric field between two electrodes present at each side of the pore, the passage of biomolecules through the pore is detected by a change in conductance (or resistance). [ 4 ] Usually the pore size is chosen to be slightly larger than the target molecule or cell.…”

Section: Introductionmentioning

confidence: 99%

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Contactless Bio‐Electrofunctionalization of Planar Micropores

Ismail

Pham

Descamps

et al. 2021

Adv Materials Technologies

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Usually the pore size is chosen to be slightly larger than the target molecule or cell. The synthetic solid state pores offer advantages over the biological pores in terms of better controlling the size and number of pores per unit area, as well as being more resistant to sensing conditions (pH, temperature, and ionic force) and easier to functionalize. [5] The best method to distinguish between two molecules having similar size is to functionalize the pore in a manner that the slowing down or capture of the target molecule in the pore has its own temporal imprint compared to the speed of other molecules. [6] This is usually obtained from the monitoring of the ionic current drop between the passage of target biomolecules and non-specific ones. Several functionalization methods have been developed including chemisorption of functional molecules through silanization or thiol-gold linkage, [7] deposition techniques (chemical and physical vapour deposition, [8] electroless deposition, [9] and atomic layer deposition), [10] chemical modification of the functional group on the nanopore to yield polymer brushes [11] or hydrogels, [12] and plasma surface modification. [13] These techniques are usually based on the functionalization of the pore and of the immediately adjacent membrane surface, which leads to capture of large amounts of molecules on the The localized functionalization of pores and channels of micrometric and sub-micrometric sizes is a bottleneck in surface chemistry. A method for the regioselective chemical functionalization of planar pores is presented, that are, restrictions in microfluidic channels, here made of SiO 2 -coated silicon. This strategy, based on bipolar electrochemistry, exploits the combined presence of the constriction and a localized deoxidation pattern within the pore that affects the electrical field distribution inside the microfluidic channel. It is not only shown that it is capable of regioselectively functionalizing a planar pore at relatively small potential difference applied across it, but also the possibility of positioning the functionalization area inside or at the edges of the pore depending on the design of the deoxidation pattern is proved. These results are in perfect correlation with the numerical simulations of electric field distribution in micropores carried out using the software Comsol Multiphysics. This functionalization technique is therefore very promising, particularly in the field of biosensors. A specific DNA hybridization test has been successfully carried out, which represents a first step toward bioanalytical and health applications.

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