Ultrahigh‐Resolution NMR Spectroscopy

Abstract

All psyched up: A flexible and general pure shift experiment (PSYCHE) has been developed that offers superior sensitivity, spectral purity, and tolerance of strong coupling over existing methods for broadband homonuclear decoupling. The partial spectra of estradiol in [D6]DMSO obtained by normal 1H NMR spectroscopy and PSYCHE are shown for comparison

High-resolution NMR spectroscopy in inhomogeneous fields

“…(27) and (28) show that 2D NMR spectra with high resolution information along the indirect domain can be obtained by the simple pulse sequences shown in Fig. 29a and b.…”

“…A strong and highly homogeneous static magnetic field is a basic requirement for high-resolution NMR. Spatial variations in magnetic field should be less than 10 -8 for a liquid high-resolution proton ( 1 H) NMR spectrum [25][26][27]. Although magnets used in modern NMR spectrometers have been carefully designed to maintain field homogeneity as high as possible, circumstances still arise in which the spatial and temporal homogeneities of magnetic fields are degraded.…”

Real-time bilinear rotation decoupling in absorptive mode J-spectroscopy: Detecting low-intensity metabolite peak close to high-intensity metabolite peak with convenience

“…Broadband approaches such as, BIRD, and ZS pulse-sequence elements, and band selective methods such as BASH or HOBS have been implemented in realtime [8,17,22] or directly detected dimension (F 2 ). PSYCHE [20,21] offers order of magnitude higher sensitivity than ZS or BIRD but is applicable only in indirect (F 1 ) dimensions of multidimensional approach. The BIRD scheme achieves refocusing of 1 H-1 H Jcouplings by exploiting the large one bond heteronuclear coupling constants in sparse heteronuclei.…”

HOBS methods for enhancing resolution and sensitivity in small DNA oligonucleotide NMR studies

“…[13,14] On the other hand, pure shift yielded by chirp excitation gives the full 1 H-{ 1 H} BB NMR spectrum reportedly with tenfold greater sensitivity compared with the spatially encoded pure shift approach. [15] HOBS is effective only if resonances from specific types of proton occur in a region of the spectrum that is relatively isolated from scalar coupling partners and allows full sensitivity to be maintained. This is a feature of particular interest when considering low concentrations of biopolymers.…”