Ultrahigh Performance Liquid Chromatography Interfaced with Mass Spectrometry and Orthogonal Ion Mobility Separation for the Microstructure Characterization of Amphiphilic Block Copolymers

Shi, Chunxiao; Gerişlioğlu, Selim; Wesdemiotis, Chrys

doi:10.1007/s10337-016-3077-1

Cited by 9 publications

(7 citation statements)

References 52 publications

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“…Conclusive differentiation of linear and cyclic structures of copolyesters and of PS‐ b ‐PEO copolymers was also achieved by Wesdemiotis and co‐workers, who took advantage in the latter case of liquid chromatography (LC) separation of co‐oligomers based on their PS block size before probing their shape by TWIMS . The same group reported, however, that the mobility resolution of this instrument is not sufficient to distinguish inter‐ and intra‐isomers of 8‐shaped PS oligomers, for which the calculated CCS values differed by less than 2% …”

Section: Architectural Distinction Of Synthetic Polymersmentioning

confidence: 99%

Ion mobility spectrometry – Mass spectrometry coupling for synthetic polymers

Charles

Chendo

Poyer

2020

Rapid Comm Mass Spectrometry

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This review covers applications of ion mobility spectrometry (IMS) hyphenated to mass spectrometry (MS) in the field of synthetic polymers. MS has become an essential technique in polymer science, but increasingly complex samples produced to provide desirable macroscopic properties of high‐performance materials often require separation of species prior to their mass analysis. Similar to liquid chromatography, the IMS dimension introduces shape selectivity but enables separation at a much faster rate (milliseconds vs minutes). As a post‐ionization technique, IMS can be hyphenated to MS to perform a double separation dimension of gas‐phase ions, first as a function on their mobility (determined by their charge state and collision cross section, CCS), then as a function of their m/z ratio. Implemented with a variety of ionization techniques, such coupling permits the spectral complexity to be reduced, to enhance the dynamic range of detection, or to achieve separation of isobaric ions prior to their activation in MS/MS experiments. Coupling IMS to MS also provides valuable information regarding the 3D structure of polymer ions in the gas phase and regarding how to address the question of how charges are distributed within the structure. Moreover, the ability of IMS to separate multiply charged species generated by electrospray ionization yields typical IMS‐MS 2D maps that permit the conformational dynamics of synthetic polymer chains to be described as a function of their length.

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Section: Architectural Distinction Of Synthetic Polymersmentioning

confidence: 99%

Ion mobility spectrometry – Mass spectrometry coupling for synthetic polymers

Charles

Chendo

Poyer

2020

Rapid Comm Mass Spectrometry

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“…Ion mobility-MS (IM-MS) counts among the most recent couplings to be probed for polymer characterizations [8][9][10][11][12][13][14][15][16][17][18][19]. Even further, MS couplings such as liquid chromatography (LC) LC-IM-MS are beginning to be investigated [20].…”

Section: Introductionmentioning

confidence: 99%

Fundamental Studies on Poly(2-oxazoline) Side Chain Isomers Using Tandem Mass Spectrometry and Ion Mobility-Mass Spectrometry

Haler

Rosa

Massonnet

et al. 2019

J. Am. Soc. Mass Spectrom.

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When polymer mixtures become increasingly complex, the conventional analysis techniques become insufficient for complete characterization. Mass spectrometric techniques can satisfy this increasing demand for detailed sample characterization. Even though isobaric polymers are indistinguishable using simple mass spectrometry (MS) analyses, more advanced techniques such as tandem MS (MS/ MS) or ion mobility (IM) can be used. Here, we report proof of concept for characterizing isomeric polymers, namely poly(2-n-propyl-2-oxazoline) (Pn-PrOx) and poly(2-isopropyl-2-oxazoline) (Pi-PrOx), using MS/MS and IM-MS. Pi-PrOx ions lose in intensity at higher accelerating voltages than Pn-PrOx ions during collision-induced dissociation (CID) MS/MS experiments. A Pn/i-PrOx mixture could also be titrated using survival yield calculations of either precursor ions or cation ejection species. IM-MS yielded shape differences in the degree of polymerization (DP) regions showing the structural rearrangements. Combined MS techniques are thus able to identify and deconvolute the molar mass distributions of the two isomers in a mixture. Finally, the MS/MS and IM-MS behaviors are compared for interpretation.

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“…Highlighted therein are reviews and original papers published in the journal during the last 2 calendar years. Should you have missed this e-mail, or should our e-mailing have (unforgivably) missed you, our readers can still find out about some of the diverse work published in Chromatographia in 2016 and 2017 in the area of polymer chromatography through the eight papers constituting the virtual issue [1][2][3][4][5][6][7][8].…”

mentioning

confidence: 99%

“…These publications exemplify the range of analytical techniques applicable to macromolecular separations, via original papers dealing with inverse gas chromatography [1], high-temperature multi-detector size-exclusion chromatography (SEC) [4], mass spectrometry and ion mobility separation [3], and asymmetric flow field-flow fractionation [5]; and in reviews dealing with off-line refractometry [7] and with SEC with on-line viscometry, in particular [2], and also covering hyphenated liquid chromatographic polymer analysis methods, in general [6].…”

mentioning

confidence: 99%