Ultrafiltration behavior of bacterial polysaccharides used in vaccines

Hadidi, Mahsa; Buckley, John J.; Zydney, Andrew L.

doi:10.1016/j.memsci.2015.04.047

Cited by 19 publications

(16 citation statements)

References 28 publications

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“…The sieving coefficients for both the free polysaccharide (left panel) and the conjugate (right panel) increase with increasing filtrate flux for all three membranes, consistent with the effects of concentration polarization (Hadidi et al, ). The solid curves are evaluated using the concentration polarization model:

S_{o} = \frac{S_{a}}{{(1 - S}_{a}) \times exp (- J_{v} / k_{m}) + S_{a}}

where k m is the bulk mass transfer coefficient in the module and S a = C p / C wall is the actual sieving coefficient, equal to the ratio of the polysaccharide concentration in the permeate solution ( C p ) to the polysaccharide concentration at the membrane surface ( C wall ).…”

Section: Results and Analysissupporting

confidence: 78%

“…Recent work by Hadidi, Buckley, and Zydney, (, ) and Emami, Motevalian, Pepin, and Zydney () have provided new insights into the factors controlling the ultrafiltration of pneumococcal polysaccharides, highlighting the important role of concentration polarization and fouling in determining the overall rate of polysaccharide transmission. In addition, Emami et al () showed that highly charged polysaccharides underwent elongation during tangential flow filtration (TFF), resulting in much greater fouling (and polysaccharide retention) in TFF cassettes compared with either stirred cell or hollow fiber modules.…”

Section: Introductionmentioning

confidence: 99%

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Purification of a conjugated polysaccharide vaccine using tangential flow diafiltration

Emami

Motevalian

Pepin

et al. 2018

Biotech & Bioengineering

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Conjugated vaccines prepared from the capsular polysaccharide of Streptococcus pneumoniae can provide immunization against invasive pneumococcal disease, meningitis, and otitis media. One of the critical steps in the production of these vaccines is the removal of free (unreacted) polysaccharides from the protein‐polysaccharide conjugate. Experimental studies were performed to evaluate the effects of membrane pore size, filtrate flux, and solution conditions on the transmission of both the conjugate and free polysaccharide through different ultrafiltration membranes. Conjugate purification was done using diafiltration performed in a linearly‐scalable tangential flow filtration cassette. More than 98% of the free polysaccharide was removed within a 5‐diavolume diafiltration process, which is a significant improvement over previously reported results for purification of similar conjugated vaccines. These results clearly demonstrate the opportunities for using ultrafiltration/diafiltration for the final purification of conjugated vaccine products.

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S_{o} = \frac{S_{a}}{{(1 - S}_{a}) \times exp (- J_{v} / k_{m}) + S_{a}}

Section: Results and Analysissupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Purification of a conjugated polysaccharide vaccine using tangential flow diafiltration

Emami

Motevalian

Pepin

et al. 2018

Biotech & Bioengineering

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“…The observed sieving coefficients increased at high flux because of concentration polarization effects as described by Hadidi et al For example, the transmission of Serotype A in the 250 mM solution increased from S o = 0.05 to 0.95 as the flux increased from 3 to 33 µm/s [11–120 L/(m 2 h)]. At any given flux, transmission of the polysaccharide decreased with decreasing ionic strength.…”

Section: Resultsmentioning

confidence: 86%

“… Hydrodynamic radius of polysaccharide Serotypes A, B, and C as measured using SEC as a function of ionic strength at pH 7. Taken from Hadidi et al…”

Section: Resultsmentioning

confidence: 99%

“…The actual sieving coefficients (

S_{a}

), which provide a true measure of the membrane retention characteristics, were evaluated from the observed transmission by accounting for the effects of concentration polarization:

ln ([]|, \frac{1}{S_{o}} - 1) = - \frac{J_{v}}{k_{m}} + ln ([]|, \frac{1}{S_{a}} - 1)

where

k_{m}

is the bulk mass transfer coefficient and

S_{a}

= C f /C w where C w is the polysaccharide concentration in the solution immediately upstream of the membrane surface. Results for Serotype A are shown in Figure at different KCl concentrations (corresponding to the observed sieving coefficient data in Figure ).…”

Section: Resultsmentioning

confidence: 99%

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Effect of electrostatic interactions on the ultrafiltration behavior of charged bacterial capsular polysaccharides

Hadidi

Buckley

Zydney

2016

Biotechnology Progress

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Charged polysaccharides are used in the food industry, as cosmetics, and as vaccines. The viscosity, thermodynamics, and hydrodynamic properties of these charged polysaccharides are known to be strongly dependent on the solution ionic strength because of both inter- and intramolecular electrostatic interactions. The goal of this work was to quantitatively describe the effect of these electrostatic interactions on the ultrafiltration behavior of several charged capsular polysaccharides obtained from Streptococcus pneumoniae and used in the production of Pneumococcus vaccines. Ultrafiltration data were obtained using various Biomax™ polyethersulfone membranes with different nominal molecular weight cutoffs. Polysaccharide transmission decreased with decreasing ionic strength primarily because of the expansion of the charged polysaccharide associated with intramolecular electrostatic repulsion. Data were in good agreement with a simple theoretical model based on solute partitioning in porous membranes, with the effective size of the different polysaccharide serotypes evaluated using size exclusion chromatography at the same ionic conditions. These results provide fundamental insights and practical guidelines for exploiting the effects of electrostatic interactions during the ultrafiltration of charged polysaccharides. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1531-1538, 2016.

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Ultrafiltration

Zydney

2018

Kirk-Othmer Encyclopedia of Chemical Technology

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Ultrafiltration is a pressure‐driven membrane process used extensively for protein concentration and desalting, for recovery and recycle of colloidal electropaints, and for removal of natural organic matter, color compounds, and viruses in wastewater treatment and the production of potable water. The performance of an ultrafiltration process is determined by the intrinsic properties of the ultrafiltration membrane, most significantly the permeability and selectivity, in combination with the effects of concentration polarization and fouling. This article reviews the key principles of ultrafiltration, including membrane casting, module design, and process configurations as well as a brief discussion of some of the more important industrial applications of ultrafiltration in bioprocessing, dairy systems, and water treatment.

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Ultrafiltration behavior of bacterial polysaccharides used in vaccines

Cited by 19 publications

References 28 publications

Purification of a conjugated polysaccharide vaccine using tangential flow diafiltration

Purification of a conjugated polysaccharide vaccine using tangential flow diafiltration

Effect of electrostatic interactions on the ultrafiltration behavior of charged bacterial capsular polysaccharides

Ultrafiltration

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