Ultrafast, low-power, PCB manufacturable, continuous-flow microdevice for DNA amplification

Kaprou, Georgia D.; Papadopoulos, Vasileios; Papageorgiou, Dimitrios; Kefala, Ioanna N.; Papadakis, George; Gizeli, Electra; Chatzandroulis, S.; Kokkoris, George; Tserepi, Angeliki

doi:10.1007/s00216-019-01911-1

Cited by 35 publications

(31 citation statements)

References 53 publications

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“…Further improvement of the herein introduced PCB-based static microPCR device can be anticipated by incorporating in the PCB, in addition to the microheaters, the microfluidic channels, as it has been demonstrated for continuous-flow microPCR [17]. This would result in a reduction of the thermal mass of the chip and thus of its energy consumption, and is planned to be implemented in the near future, in combination with active cooling, for the next generation of miniaturized PCB thermocyclers.…”

Section: Discussionmentioning

confidence: 99%

“…The effect of the footprint on the power consumption of microPCR devices has been demonstrated through detailed numerical calculations [19]. Despite the fact that the power consumption is slightly larger than those reported in continuous-flow microPCR devices realized in thin polyimide (2.4 W [13]) or PCB (2.7 W [17]), it is far smaller than the power consumption of conventional thermocyclers (which is 500 W or higher [26]). This constitutes the main advantage of the proposed PCB-based miniaturized thermocyclers, combined with their ease of fabrication in large-scale, by leveraging the established and widespread PCB industry.…”

Section: Temperature Uniformity On Pcb Microheater Chipsmentioning

confidence: 99%

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Towards PCB-Based Miniaturized Thermocyclers for DNA Amplification

et al. 2020

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In recent years, printed circuit board (PCB)-based microfluidics have been explored as a means to achieve standardization, seamless integration, and large-scale manufacturing of microfluidics, thus paving the way for widespread commercialization of developed prototypes. In this work, static micro polymerase chain reaction (microPCR) devices comprising resistive microheaters integrated on PCBs are introduced as miniaturized thermocyclers for efficient DNA amplification. Their performance is compared to that of conventional thermocyclers, in terms of amplification efficiency, power consumption and duration. Exhibiting similar efficiency to conventional thermocyclers, PCB-based miniaturized thermocycling achieves faster DNA amplification, with significantly smaller power consumption. Simulations guide the design of such devices and propose means for further improvement of their performance.

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Section: Discussionmentioning

confidence: 99%

Section: Temperature Uniformity On Pcb Microheater Chipsmentioning

confidence: 99%

Towards PCB-Based Miniaturized Thermocyclers for DNA Amplification

et al. 2020

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“…Recently, LoC technology has also been applied in the detection of antibioticresistant bacteria [3]. Some of the advantages offered by the LoC technology compared to macro-scale methods are: fast and high throughput analysis, accurate fluid manipulation, low cost, low reagent, and power consumption, smaller sample volume, automation, integration, compactness, and portability [149][150][151][152]. Genotypic and phenotypic assays are the two main categories of microfluidic-based detection methods.…”

Section: Microfluidics and Lab-on-a-chip Technologies Towards Rapid Dmentioning

confidence: 99%

Rapid Methods for Antimicrobial Resistance Diagnostics

et al. 2021

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Antimicrobial resistance (AMR) is one of the most challenging threats in public health; thus, there is a growing demand for methods and technologies that enable rapid antimicrobial susceptibility testing (AST). The conventional methods and technologies addressing AMR diagnostics and AST employed in clinical microbiology are tedious, with high turnaround times (TAT), and are usually expensive. As a result, empirical antimicrobial therapies are prescribed leading to AMR spread, which in turn causes higher mortality rates and increased healthcare costs. This review describes the developments in current cutting-edge methods and technologies, organized by key enabling research domains, towards fighting the looming AMR menace by employing recent advances in AMR diagnostic tools. First, we summarize the conventional methods addressing AMR detection, surveillance, and AST. Thereafter, we examine more recent non-conventional methods and the advancements in each field, including whole genome sequencing (WGS), matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) spectrometry, Fourier transform infrared (FTIR) spectroscopy, and microfluidics technology. Following, we provide examples of commercially available diagnostic platforms for AST. Finally, perspectives on the implementation of emerging concepts towards developing paradigm-changing technologies and methodologies for AMR diagnostics are discussed.

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“…Recently, the PCB-based thermocycler for PCR developed in [35] (2019) required a syringe pump in order to move the DNA sample through a microchannel; the lab-on-PCB reported in [36] (2019) for organotypic cultures required continuous flow. Therefore, it needed to be connected to a external source (New Era Pump Systems, Inc, Farmingdale, NY, USA) to feed the tissues with culture medium.…”

Section: External Energy Systemsmentioning

confidence: 99%

Lab-on-PCB and Flow Driving: A Critical Review

Perdigones

2021

Micromachines

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Lab-on-PCB devices have been developed for many biomedical and biochemical applications. However, much work has to be done towards commercial applications. Even so, the research on devices of this kind is rapidly increasing. The reason for this lies in the great potential of lab-on-PCB devices to provide marketable devices. This review describes the active flow driving methods for lab-on-PCB devices, while commenting on their main characteristics. Among others, the methods described are the typical external impulsion devices, that is, syringe or peristaltic pumps; pressurized microchambers for precise displacement of liquid samples; electrowetting on dielectrics; and electroosmotic and phase-change-based flow driving, to name a few. In general, there is not a perfect method because all of them have drawbacks. The main problems with regard to marketable devices are the complex fabrication processes, the integration of many materials, the sealing process, and the use of many facilities for the PCB-chips. The larger the numbers of integrated sensors and actuators in the PCB-chip, the more complex the fabrication. In addition, the flow driving-integrated devices increase that difficulty. Moreover, the biological applications are demanding. They require transparency, biocompatibility, and specific ambient conditions. All the problems have to be solved when trying to reach repetitiveness and reliability, for both the fabrication process and the working of the lab-on-PCB, including the flow driving system.

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Ultrafast, low-power, PCB manufacturable, continuous-flow microdevice for DNA amplification

Cited by 35 publications

References 53 publications

Towards PCB-Based Miniaturized Thermocyclers for DNA Amplification

Towards PCB-Based Miniaturized Thermocyclers for DNA Amplification

Rapid Methods for Antimicrobial Resistance Diagnostics

Lab-on-PCB and Flow Driving: A Critical Review

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