Ultrafast Laser Biomimetic Micro-/Nanostructuring

Tsibidis, George D.; Stratakis, Emmanuel

doi:10.1007/978-3-031-14752-4_25

Cited by 3 publications

(1 citation statement)

References 78 publications

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“…The transfer of such bioinspired "natural" nano-and microstructures is possible with ultrashort laser pulses (ultrafast lasers) on metals (steel and titanium alloy) [29,30]. Until now, however, the production of well-defined structures on this scale has not been possible, so attempts at etching or other randomized methods in wound healing have been unsuccessful [31][32][33].…”

Section: Introductionmentioning

confidence: 99%

The Impact of Ultrashort Pulse Laser Structuring of Metals on In-Vitro Cell Adhesion of Keratinocytes

Staehlke,

Barth,

Muench

et al. 2024

JFB

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Besides the need for biomaterial surface modification to improve cellular attachment, laser-structuring is favorable for designing a new surface topography for external bone fixator pins or implants. The principle of this study was to observe how bioinspired (deer antler) laser-induced nano–microstructures influenced the adhesion and growth of skin cells. The goal was to create pins that allow the skin to attach to the biomaterial surface in a bacteria-proof manner. Therefore, typical fixator metals, steel, and titanium alloy were structured using ultrashort laser pulses, which resulted in periodical nano- and microstructures. Surface characteristics were investigated using a laser scanning microscope and static water contact angle measurements. In vitro studies with human HaCaT keratinocytes focused on cell adhesion, morphology, actin formation, and growth within 7 days. The study showed that surface functionalization influenced cell attachment, spreading, and proliferation. Micro-dimple clusters on polished bulk metals (DC20) will not hinder viability. Still, they will not promote the initial adhesion and spreading of HaCaTs. In contrast, additional nanostructuring with laser-induced periodic surface structures (LIPSS) promotes cell behavior. DC20 + LIPSS induced enhanced cell attachment with well-spread cell morphology. Thus, the bioinspired structures exhibited a benefit in initial cell adhesion. Laser surface functionalization opens up new possibilities for structuring, and is relevant to developing bioactive implants in regenerative medicine.

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