Ultrafast FRET at fiber tips: Potential applications in sensitive remote sensing of molecular interaction

Polley, Nabarun; Singh, Soumendra; Giri, Anupam; Mondal, Prasanna Kumar; Lemmens, P.; Pal, Samir Kumar

doi:10.1016/j.snb.2014.12.129

Cited by 19 publications

(12 citation statements)

References 24 publications

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“…28–32 Additional advances include highly advanced techniques such as atomic force microscopy supplemented with FRET (AFM-FRET) 33 and time-resolved measurements with nanosecond resolution. 34 These innovations have made FRET an easily accessible – and widely applicable – technique for studying molecular interactions. Here, we review the various ways in which FRET can be deployed to study supramolecular systems.…”

Section: Introductionmentioning

confidence: 99%

Investigating supramolecular systems using Förster resonance energy transfer

et al. 2018

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Supramolecular systems have applications in areas as diverse as materials science, biochemistry, analytical chemistry, and nanomedicine. However, analyzing such systems can be challenging due to the wide range of time scales, binding strengths, distances, and concentrations at which non-covalent phenomena take place. Due to their versatility and sensitivity, Förster resonance energy transfer (FRET)-based techniques are excellently suited to meet such challenges. Here, we detail the ways in which FRET has been used to study non-covalent interactions in both synthetic and biological supramolecular systems. Among other topics, we examine methods to measure molecular forces, determine protein conformations, monitor assembly kinetics, and visualize in vivo drug release from nanoparticles. Furthermore, we highlight multiplex FRET techniques, discuss the field's limitations, and provide a perspective on new developments.

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Section: Introductionmentioning

confidence: 99%

Investigating supramolecular systems using Förster resonance energy transfer

et al. 2018

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“…A special type of these sensors uses the so-called FRET (Förster Resonance energy transfer) as the sensing mechanism [6,7].…”

Section: Introductionmentioning

confidence: 99%

Polymer optical fiber compound parabolic concentrator tip for enhanced coupling efficiency for fluorescence based glucose sensors

Hassan¹,

Nielsen

Aasmul³

et al. 2015

Biomed. Opt. Express

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Abstract:We demonstrate that the light excitation and capturing efficiency of fluorescence based fiber-optical sensors can be significantly increased by using a CPC (Compound Parabolic Concentrator) tip instead of the standard plane-cut tip. We use Zemax modelling to find the optimum CPC tip profile and fiber length of a polymer optical fiber diabetes sensor for continuous monitoring of glucose levels. We experimentally verify the improved performance of the CPC tipped sensor and the predicted production tolerances. Due to physical size requirements when the sensor has to be inserted into the body a non-optimal fiber length of 35 mm is chosen. For this length an average improvement in efficiency of a factor of 1.7 is experimentally demonstrated and critically compared to the predicted ideal factor of 3 in terms of parameters that should be improved through production optimization.

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“…Recently the interest in fluorescence based fiber-optical sensors has increased, as they can be used for measuring This various physical and biological quantities, such as aluminum ions [5], glucose [3,6,7], α-streptavidin and α-CRP antibodies [8][9][10], and salmonella bacteria [11]. A special type of these sensors uses so-called FRET (Förster Resonance energy transfer) as the sensing mechanism [3,6,7,11,12]. FRET is an intermolecular phenomenon of radiation less energy transfer between an excited donor fluorophore and a proximal ground state acceptor (a fluorophore or a dye) through a long range dipole-dipole interaction [13] and it can be used for the measurement of intermolecular distances in the range of Ångstrøms (10-100Å) [14].…”

Section: Introductionmentioning

confidence: 99%

Polymer Optical Fiber Compound Parabolic Concentrator fiber tip based glucose sensor: in-Vitro Testing

et al. 2016

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We present in-vitro sensing of glucose using a newly developed efficient optical fiber glucose sensor based on a Compound Parabolic Concentrator (CPC) tipped polymer optical fiber (POF). A batch of 9 CPC tipped POF sensors with a 35 mm fiber length is shown to have an enhanced fluorescence pickup efficiency with an average increment factor of 1.7 as compared to standard POF sensors with a plane cut fiber tip. Invitro measurements for two glucose concentrations (40 and 400 mg/dL) confirm that the CPC tipped sensors efficiently can detect both glucose concentrations.

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Ultrafast FRET at fiber tips: Potential applications in sensitive remote sensing of molecular interaction

Cited by 19 publications

References 24 publications

Investigating supramolecular systems using Förster resonance energy transfer

Investigating supramolecular systems using Förster resonance energy transfer

Polymer optical fiber compound parabolic concentrator tip for enhanced coupling efficiency for fluorescence based glucose sensors

Polymer Optical Fiber Compound Parabolic Concentrator fiber tip based glucose sensor: in-Vitro Testing

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