Ultradeformable archaeosomes as new topical adjuvants

Higa, Leticia Herminia; Schilrreff, Priscila; Perez, Ana Paula; Iriarte, Maiara A.; Roncaglia, Diana I.; Morilla, María José; Romero, Eder Lilia

doi:10.1016/j.nano.2012.02.008

Cited by 51 publications

(34 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The present findings are in agreement with the results of Gonzalez et al that prepared archaeosomes from total polar lipids of Halorubrum tebenquichense and trailed them in J774 cells (murine macrophages) using a hydrophilic dye pyranine (HPTS: 8-hydroxypyrene-1, 3, 6-trisulfonic acid). Their results showed that the archaeosomes did not fuse or disrupt staying inside the phagosomes for at least 60 min [16]. However, our findings are not in agreement with Higa et al (2012) who evaluated cell uptake of HPTS-containing ultradeformable archaeosomes (UDA); a type of vesicles that made of soybean phosphatidylcholine, sodium cholate, and polar lipids from Halorubrum tebenquichense.…”

Section: Discussioncontrasting

confidence: 99%

Formulation of a New Generation of Liposomes from Bacterial and Archeal Lipids

Ameri¹,

Moghimipour²,

Ramezani³

et al. 2016

Trop. J. Pharm Res

View full text Add to dashboard Cite

Section: Discussioncontrasting

confidence: 99%

Formulation of a New Generation of Liposomes from Bacterial and Archeal Lipids

Ameri¹,

Moghimipour²,

Ramezani³

et al. 2016

Trop. J. Pharm Res

View full text Add to dashboard Cite

“…Ultra deformable archaeosomes (UDA) are vesicles made of soybean phosphatidylcholine (SPC), sodium cholate (NaChol) and polar lipids from Halorubrum tebenquichense (3:1:3 wt/wt). UDA were prepared by lipid hydration method for topical applications (Higa et al, 2012).…”

Section: Lipid Hydration Methodsmentioning

confidence: 99%

“…Topical vaccination has more advantages than injectable, such as increased patient compliance, the reduced potential reinfection by contaminated material, and as well as the need for particular trained personnel and sterilized materials, and maintenance of cold chain. These advantages are more or less outweighed by dose variability and the need for strong immunomodulators, that is bacterial ADP-ribosylating exotoxins (cholera toxin, Escherichia coli and their mutants) (Higa et al, 2012). UDA are vesicles prepared from SPC, NaChol and polar lipids from H. tebenquichense (3:1:3wt/wt).…”

Section: Uda As Topical Adjuvantmentioning

confidence: 99%

Archaeosomes: an excellent carrier for drug and cell delivery

et al. 2015

View full text Add to dashboard Cite

Archaeosomes as liposomes made with one or more ether lipids that are unique to the domain of Archaeobacteria, found in Archaea constitute a novel family of liposome. Achaean-type lipids consist of archaeol (diether) and/or caldarchaeol (tetraether) core structures. Archaeosomes can be produced using standard procedures (hydrated film submitted to sonication, extrusion and detergent dialysis) at any temperature in the physiological range or lower, therefore making it possible to encapsulate thermally stable compounds. Various physiological as well as environmental factors affect its stability. Archaeosomes are widely used as drug delivery systems for cancer vaccines, Chagas disease, proteins and peptides, gene delivery, antigen delivery and delivery of natural antioxidant compounds. In this review article, our major aim was to explore the applications of this new carrier system in pharmaceutical field.

show abstract

“…38 Between 90 mg and 120 mg TPAs were isolated from each culture batch. The reproducibility of each TPA-extract composition was routinely screened by phosphate content 39 and electrospray-ionization mass spectrometry, as described in Higa et al 36 …”

Section: Methodsmentioning

confidence: 99%

“…The UDAs were successfully used as antigen carriers for topical vaccination, because of their deep skin penetration and higher phagocytic uptake than UDLs, improving the antigen delivery to skin antigen-presenting cells. 36 We hypothesized that TPAs containing liposomes could be used to increase the targeted delivery of ZnPc to macrophages to a higher extent than UDLs. Therefore, in this work, a commercial hydrophobic ZnPc was loaded into TPAs containing liposomes, and its in vitro leishmanicidal activity was compared to that of ZnPc loaded in UDLs on macrophages infected with L. braziliensis amastigotes.…”

Section: Introductionmentioning

confidence: 99%

Enhanced photodynamic leishmanicidal activity of hydrophobic zinc phthalocyanine within archaeolipids containing liposomes

Perez

Casasco

Schilrreff

et al. 2014

IJN

Self Cite

View full text Add to dashboard Cite

In this work, the in vitro anti-Leishmania activity of photodynamic liposomes made of soybean phosphatidylcholine, sodium cholate, total polar archaeolipids (TPAs) extracted from the hyperhalophile archaea Halorubrum tebenquichense and the photosensitizer zinc phthalocyanine (ZnPcAL) was compared to that of ultradeformable photodynamic liposomes lacking TPAs (ZnPcUDLs). We found that while ZnPcUDLs and ZnPcALs (130 nm mean diameter and −35 mV zeta potential) were innocuous against promastigotes, a low concentration (0.01 µM ZnPc and 7.6 µM phospholipids) of ZnPcALs irradiated at a very low-energy density (0.2 J/cm 2 ) eliminated L. braziliensis amastigotes from J774 macrophages, without reducing the viability of the host cells. In such conditions, ZnPcALs were harmless for J774 macrophages, HaCaT keratinocytes, and bone marrow-derived dendritic cells. Therefore, topical photodynamic treatment would not likely affect skin-associated lymphoid tissue. ZnPcALs were extensively captured by macrophages, but ZnPcUDLs were not, leading to 2.5-fold increased intracellular delivery of ZnPc than with ZnPcUDLs. Despite mediating low levels of reactive oxygen species, the higher delivery of ZnPc and the multiple (caveolin-and clathrin-dependent plus phagocytic) intracellular pathway followed by ZnPc would have been the reason for the higher antiamastigote activity of ZnPcALs. The leishmanicidal activity of photodynamic liposomal ZnPc was improved by TPA-containing liposomes.

show abstract

Ultradeformable archaeosomes as new topical adjuvants

Cited by 51 publications

References 45 publications

Formulation of a New Generation of Liposomes from Bacterial and Archeal Lipids

Formulation of a New Generation of Liposomes from Bacterial and Archeal Lipids

Archaeosomes: an excellent carrier for drug and cell delivery

Enhanced photodynamic leishmanicidal activity of hydrophobic zinc phthalocyanine within archaeolipids containing liposomes

Contact Info

Product

Resources

About