Ultracytochemistry of glycosylation sites of rat colonic mucous cells with labeled lectins.

Tsuyama, Shinichiro; Suganuma, Tatsuo; Ihida, Kaori; Murata, Fusayoshi

doi:10.1267/ahc.19.555

Cited by 8 publications

(7 citation statements)

References 33 publications

(6 reference statements)

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“…It has generally been agreed that lectins specific to α-GalNAc bind to cis-stacks of the Golgi apparatus in cells actively secreting O-glycosylated glycoproteins (Roth 1984;Tsuyama et al 1986;Pavelka and Ellinger 1991;Suzaki and Kataoka 1992). This is also confirmed by the results of our preliminary electron microscopic examination on binding sites of colloidal-gold labeled HAA in the mouse descending colon.…”

Section: Discussionsupporting

confidence: 84%

“…Lectins make good markers of the Golgi stacks and mucous granules in cells actively secreting O-glycosylated glycoproteins, such as goblet cells and Brunner's gland cells (Roth 1984;Tsuyama et al 1986;Pavelka 1988, 1992;Pavelka and Ellinger 1991;Suzaki and Kataoka 1992). Lectins specific to α-N-acetylgalactosamine (GalNAc), such as Glycine max agglutinin (SBA), Helix pomatia agglutinin, and Maclura pomifera agglutinin (MPA), usually bind to cis-stacks of the Golgi apparatus, whereas Ulex europaeus agglutinin I (UEA I) specific to α-fucose, a terminal sugar residue, binds to trans-stacks and to mucous granules.…”

Section: Introductionmentioning

confidence: 99%

“…Lectins specific to α-N-acetylgalactosamine (GalNAc), such as Glycine max agglutinin (SBA), Helix pomatia agglutinin, and Maclura pomifera agglutinin (MPA), usually bind to cis-stacks of the Golgi apparatus, whereas Ulex europaeus agglutinin I (UEA I) specific to α-fucose, a terminal sugar residue, binds to trans-stacks and to mucous granules. In addition, lectins specific to α-GalNAc bind to trans-stacks and mucous granules in some cells that produce glycoproteins with α-GalNAc as a terminal sugar residue (Roth 1984;Tsuyama et al 1986;Pavelka and Ellinger 1991;Suzaki and Kataoka 1992).…”

Section: Introductionmentioning

confidence: 99%

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The Golgi apparatus of goblet cells in the mouse descending colon: three-dimensional visualization using a confocal laser scanning microscope

Kataoka

Suzaki

Komura

2001

Histochem Cell Biol

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The three-dimensional structure of the Golgi apparatus was studied in goblet cells in lectin-stained sections of the mouse descending colon by using a confocal laser scanning microscope. In the lower part of the crypt, the Golgi apparatus formed a dome- or globe-like structure in the supranuclear region. The wall of the dome had some holes, one of which usually faced toward the nucleus and others toward the apical cytoplasm. Mucous granules seemed to be initially released into the interior of the dome and transported toward the apical cytoplasm through the holes. In the upper part of the crypt, on the other hand, the Golgi apparatus formed a cup- or funnel-like structure with a larger opening toward the cell apex and a smaller opening toward the nucleus. A large mass of mucous granules occupied the inside of the cup to the apical cytoplasm. It is thought that the accumulation of mucous granules enlarges holes at the ceiling of the dome to form a large opening, which makes the configuration of the Golgi apparatus cup-shaped.

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Section: Discussionsupporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Golgi apparatus of goblet cells in the mouse descending colon: three-dimensional visualization using a confocal laser scanning microscope

Kataoka

Suzaki

Komura

2001

Histochem Cell Biol

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“…These findings suggest a direct relation between the location of goblet cells and their carbohydrate contents. However, Tsuyama et al (41,43) have reported the presence of goblet cells at the same depth of the rat intestinal glands which showed different stainabilities.…”

Section: Gallus Gallus (Chicken) Small Intestinementioning

confidence: 99%

“…Recently, lectins have been found to be powerful and reliable tools to investigate the characteristics and distribution of glycoproteins in tissues. They have been widely employed to describe the tissue distribution of carbohydrates in the human and rat intestinal mucosa, both at light and electron microscopic levels (3,7,8,13,14,17,25,26,28,30,34,35,(41)(42)(43)(44)(45). In the intestinal mucosa of non-mammalian vertebrates, however, lectins have been applied only to the chicken colon (40).…”

mentioning

confidence: 99%

Characterization of glycoconjugates in the intestinal mucosa of vertebrates by means of lectin histochemistry.

Madrid

Ballesta

Castells

et al. 1989

Acta Histochem. Cytochem

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The glycoproteins of the intestinal mucosa of five vertebrate species including fishes (Sparus auratus), amphibians (Rang perezi), reptiles (Testudo graeca), birds (Gallus gallus) and mammals (Mesocricetus auratus) were studied by means of methods of lectin histochemistry. Goblet cells of the intestine showed different lectin binding patterns depending upon the location in the villi. Thus, goblet cells located in the intestinal glands presented a different reactivity from that of the goblet cells located at the tip of the villi. Differences in lectin stainings between small and large intestines were more marked in higher than in lower vertebrates. WGA binding sites were the most abundant throughout the intestinal mucosa of the animals studied. Golgi areas of the different cell types studied were frequently reactive for DBA and SBA. The results obtained in the Brunner's glands of the hamster were similar to those obtained in other mammals, suggesting a common mucin composition. In the hamster intestinal glands, Con-A stained the Paneth cells.

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Quantitative Study of Changes in Intestinal Morphology and Mucus Gel on Total Parenteral Nutrition in Rats

Sakamoto¹,

Hirose²,

Onizuka³

et al. 2000

Journal of Surgical Research

204

120

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Ultracytochemistry of glycosylation sites of rat colonic mucous cells with labeled lectins.

Cited by 8 publications

References 33 publications

The Golgi apparatus of goblet cells in the mouse descending colon: three-dimensional visualization using a confocal laser scanning microscope

The Golgi apparatus of goblet cells in the mouse descending colon: three-dimensional visualization using a confocal laser scanning microscope

Characterization of glycoconjugates in the intestinal mucosa of vertebrates by means of lectin histochemistry.

Quantitative Study of Changes in Intestinal Morphology and Mucus Gel on Total Parenteral Nutrition in Rats

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