Ultra-trace element analysis of human follicular fluid by ICP-MS/MS: pre-analytical challenges, contamination control, and matrix effects

Galusha, Aubrey L.; Haig, Aubreian C; Bloom, Michael S.; Kruger, Pamela C.; McGough, Alexandra; Lenhart, Nikolaus; Wong, Rebecca S. Y.; Fujimoto, Victor Y.; Mok-Lin, Evelyn; Parsons, Patrick J.

doi:10.1039/c8ja00423d

Cited by 13 publications

(9 citation statements)

References 21 publications

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“…Between 0.5 mL and 5.0 mL FF was aspirated from a single large follicle in each ovary. The follicular fluid was transferred to a sterile Petri dish, and after the oocyte removal, the fluid was placed into a 15 mL conical tube and centrifuged for 5 minutes at 1500 g, distributed into 1.8 mL polypropylene cryo-vials, and stored at 80 • C until further use (11) .…”

Section: Follicular Fluid Preparation and Freezingmentioning

confidence: 99%

Effect of passive smoking on heavy metals concentration in blood and follicular fluid of patients on going ICSI

ElMohr¹,

Faris²,

Bakry³

et al. 2020

IJST

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Purpose: To assess the effects passive smoking and heavy metal concentrations in the blood and follicular fluid on assisted reproductive technology outcome.Methods: A prospective study was conducted between March 2017 and January 2018 in population consisted of 75 female patients undergoing an In vitro fertilization (IVF) cycle at Dr. Faris Medical center for Infertility and Human Reproduction located on Heliopolis, Cairo, Egypt; with unexplained infertility who underwent intracytoplasmic sperm injection (ICSI) using GnRH-antagonist protocol. Concentrations of three toxic metals Cadmium (Cd), Lead (Pb) and Arsenic (As) were measured both in blood sera and follicular fluid specimens. Patients were evaluated in two groups both undergoing ICSI; the first group consisted of patients who does not smoke or exposed to smoking (n=28) and the group passive smoker females (n=47). Results: These three heavy metals were significantly higher in serum and follicular fluid of passive smoking females (P<0.05). Also, higher concentrations of Cd, Pb and As were found in follicular fluid of passive smoking patients in comparison with non-smoker females. However, concentrations of Cd and Pb were not significantly different between both serum and follicular fluid. Conclusion: Passive smoking could affect levels of Cd, Pb and As in serum and follicular fluid of females undergoing Intra Cytoplasmic Sperm Injection (ICSI).

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Section: Follicular Fluid Preparation and Freezingmentioning

confidence: 99%

Effect of passive smoking on heavy metals concentration in blood and follicular fluid of patients on going ICSI

ElMohr¹,

Faris²,

Bakry³

et al. 2020

IJST

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“…One FF aliquot from each sample (n = 197) was analysed for As, Hg, Cd and Pb, at the Laboratory of Inorganic and Nuclear Chemistry, Wadsworth Center, New York State Department of Health (Albany, NY, USA). The analytical method, described in detail in a previous publication ( Galusha et al ., 2019 ), was developed on an Agilent 8900 inductively coupled plasma tandem mass spectrometer (ICP-MS/MS) (Agilent Technologies, Santa Clara, CA, USA). Briefly, specimens were prepared along with standards and quality control samples (QCs), diluted into polypropylene autosampler tubes and analysed by ICP-MS/MS.…”

Section: Methodsmentioning

confidence: 99%

Toxic elements in follicular fluid adversely influence the likelihood of pregnancy and live birth in women undergoing IVF

Butts

Bloom

McGough

et al. 2021

Human Reproduction Open

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STUDY QUESTION Are follicular fluid (FF), arsenic (As), mercury (Hg), cadmium (Cd) and lead (Pb) concentrations associated with IVF outcomes among women undergoing IVF? SUMMARY ANSWER There was a non-linear association between higher FF Hg concentration and a lower likelihood of biochemical pregnancy and live birth. Higher FF Pb concentration was also associated with a lower probability of live birth. WHAT IS KNOWN ALREADY Previous research suggests that toxic elements may affect fertility among couples conceiving with and without assistance. However, the results have been inconsistent, possibly related in part to exposure misclassification. Very few studies have used ovarian FF to measure toxic elements, as it requires an invasive collection procedure, yet it may offer a more accurate estimate of a biologically effective dose than blood or urine. STUDY DESIGN, SIZE, DURATION This is a prospective study of 56 women undergoing IVF, from October 2015 to June 2017. FF was collected for analysis on the day of oocyte retrieval. PARTICIPANTS/MATERIALS, SETTING, METHODS As, Cd, Hg and Pb were determined in 197 FF specimens, using inductively coupled plasma tandem mass spectrometry. FF glutathione peroxidase, glutathione reductase, total glutathione-S-transferase, superoxide dismutase, arylesterase and paraoxonase (PON1p) activities were measured using kinetic enzyme assays. MAIN RESULTS AND THE ROLE OF CHANCE Non-linear associations were detected, in which the probabilities of biochemical pregnancy (P = 0.05) and live birth (P = 0.05) were lower in association with FF Hg greater than ∼0.51 µg/l Hg, adjusted for age, race, cigarette smoking and recent seafood consumption. Higher FF Pb was also associated with a lower likelihood of live birth (relative risk (RR) = 0.68, 95% CI: 0.46, 1.00; P = 0.05). We also found a suggestive, although imprecise, antagonizing mediating effect of PON1p activity on the association between FF Pb and live birth (−28.3%; 95% CI: −358%, 270%). LIMITATIONS, REASONS FOR CAUTION The results should be interpreted judiciously given the limited sample size and difficulty accounting for correlated data in generalized additive models and mediation analyses. Additionally, women undergoing IVF are highly selected with respect to age and socioeconomic status, and so the generalizability of the results may be limited. WIDER IMPLICATIONS OF THE FINDINGS Overall, the results suggest that FF Hg was associated with a lower likelihood of biochemical pregnancy and live birth, with a potential threshold effect, and that higher FF Pb was associated with a lower probability of live birth. These results may help to guide clinical recommendations for limiting the exposure of patients to Hg and Pb and ultimately improve IVF success rates. STUDY FUNDING/COMPETING INTEREST(S) This work was funded in part by the National Institute of Environmental Health Sciences (NIEHS), grant number 1R56ES023886-01, to the University at Albany (M.S.B.), and in part by the National Institute of Environmental Health Sciences (NIEHS), grant number 1U2CES026542-01, to the Wadsworth Center (P.J.P.). The authors declare no competing interests. TRIAL REGISTRATION NUMBER N/A

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“…It is worth emphasising that besides the mentioned biofluids and tissues, virtually any biospecimen can be interrogated in ICP-MS regarding elemental profiles and a disrupted homeostasis. In the recent years, a range of studies started exploring rather untraditional specimens including faeces [ 80 ], tears [ 81 ], milk [ 82 ], breath [ 83 ], skin [ 84 ], bone marrow fluid [ 85 ], nasal exudate [ 86 ], follicular fluid [ 87 ] or seminal plasma [ 88 ] to just name a few examples. Especially microsampling of biological fluids seems to become more relevant to ensure minimal invasive procedures and to target scarce fluids.…”

Section: Stand-alone Icp-msmentioning

confidence: 99%

Facets of ICP-MS and their potential in the medical sciences—Part 1: fundamentals, stand-alone and hyphenated techniques

Clases

Vega

2022

Anal Bioanal Chem

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Since its inception in the early 80s, inductively coupled plasma–mass spectrometry has developed to the method of choice for the analysis of elements in complex biological systems. High sensitivity paired with isotopic selectivity and a vast dynamic range endorsed ICP-MS for the inquiry of metals in the context of biomedical questions. In a stand-alone configuration, it has optimal qualities for the biomonitoring of major, trace and toxicologically relevant elements and may further be employed for the characterisation of disrupted metabolic pathways in the context of diverse pathologies. The on-line coupling to laser ablation (LA) and chromatography expanded the scope and application range of ICP-MS and set benchmarks for accurate and quantitative speciation analysis and element bioimaging. Furthermore, isotopic analysis provided new avenues to reveal an altered metabolism, for the application of tracers and for calibration approaches. In the last two decades, the scope of ICP-MS was further expanded and inspired by the introduction of new instrumentation and methodologies including novel and improved hardware as well as immunochemical methods. These additions caused a paradigm shift for the biomedical application of ICP-MS and its impact in the medical sciences and enabled the analysis of individual cells, their microenvironment, nanomaterials considered for medical applications, analysis of biomolecules and the design of novel bioassays. These new facets are gradually recognised in the medical communities and several clinical trials are underway. Altogether, ICP-MS emerged as an extremely versatile technique with a vast potential to provide novel insights and complementary perspectives and to push the limits in the medical disciplines. This review will introduce the different facets of ICP-MS and will be divided into two parts. The first part will cover instrumental basics, technological advances, and fundamental considerations as well as traditional and current applications of ICP-MS and its hyphenated techniques in the context of biomonitoring, bioimaging and elemental speciation. The second part will build on this fundament and describe more recent directions with an emphasis on nanomedicine, immunochemistry, mass cytometry and novel bioassays. Graphical abstract

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Ultra-trace element analysis of human follicular fluid by ICP-MS/MS: pre-analytical challenges, contamination control, and matrix effects

Abstract: Human follicular fluid was collected from 56 women undergoing IVF treatment and analyzed for 11 (ultra-)trace elements using a “dilute and shoot” ICP-MS/MS method.

Cited by 13 publications

References 21 publications

Effect of passive smoking on heavy metals concentration in blood and follicular fluid of patients on going ICSI

Effect of passive smoking on heavy metals concentration in blood and follicular fluid of patients on going ICSI

Toxic elements in follicular fluid adversely influence the likelihood of pregnancy and live birth in women undergoing IVF

Facets of ICP-MS and their potential in the medical sciences—Part 1: fundamentals, stand-alone and hyphenated techniques

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