Ultra-trace determination of beryllium in occupational hygiene samples by ammonium bifluoride extraction and fluorescence detection using hydroxybenzoquinoline sulfonate

The bioinorganic chemistry and associated immunology of chronic beryllium disease

“…HBQS is an extraordinary ligand even at pH 12 and is part of a commercial beryllium detection system that can selectively detect beryllium at concentrations <1 nM. 35,36 In order to understand if there was any commonality between the binding of monomer and polynuclear binding ligands we re-examined the binding of Be to a wide variety of monomeric ligands. After considering Be binding across a wide range of ligands and careful reexamination of the proton NMR spectra of the polynuclear ligands in aprotic media, we have proposed a new binding concept that focuses on the comparison of beryllium to H + .…”

High Sensitivity Low Fluorescence Detection for Beryllium Particulates SBIR Phase I Final Report ER84587

“…Experiments with the dye structure were done in collaboration with Las Alamos National Lab in collaboration with Dr. Mark McCleskey and Dr. Anthony Burrell, and the inter-lab study with dilution ratios was performed in collaboration with Dr. Kevin Ashley at NIOSH. The results from the dilution ratios reported here were published recently 12 and have also been incorporated in the recently approved NIOSH procedures 7704 13 and 9110 14 . The ASTM method 7202-05 has already been modified to include this and is now published as ASTM D7202-06 15 .…”

Beryllium Measurement in Commercially Available Wet Wipes

“…Because of the large size of the wipes, 20 ml of ABF was chosen to get complete immersion of the wipe so that beryllium could be effectively extracted in the dissolution solution. The liquid, after extraction, was then analyzed using 5 X dilution [2] where 0.4 ml of the dissolution solution was added to 1.6 ml of a detection solution containing 1.1mM HBQS (hydroxybenzoquinoline sulfonate), 1 mM ethylenediamine tetraacetic acid and 100 mM L-Lysine monochloride with the solution pH adjusted to 12.85 (Berylliant, Inc., Tucson, AZ). The fluorescence measurements for beryllium quantification were performed using a Turner BioSystems Modulus fluorometer (Sunnyvale, CA) with a bandpass filter for excitation and emission.…”